Next Article in Journal
Previous Article in Journal
Molecules 2013, 18(9), 11299-11310; doi:10.3390/molecules180911299
Article

Isolation and Identification of Two Novel Attractant Compounds from Chinese Cockroach (Eupolyphaga sinensis Walker) by Combination of HSCCC, NMR and CD Techniques

1
, 2
 and 1,*
Received: 5 August 2013; in revised form: 6 September 2013 / Accepted: 6 September 2013 / Published: 13 September 2013
Download PDF [234 KB, uploaded 18 June 2014]
Abstract: High-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hextane-ethyl acetate-methanol-water (1.5:1:1.5:1, v/v/v/v) was applied to the isolation and purification of attractants from Chinese cockroach, Eupolyphaga sinensis Walker. Two new attractants with attractant activity towards the male insects were obtained from the extract sample in a one-step separation. Their purities were determined by HPLC. Subsequent MS, NMR and CD analyses have led to the characterization of (R)-3-ethyl-6,8-dihydroxy-7-methyl-3,4-dihydroisochromen-1-one (1) and (R)-6,8-dihydroxy-3,7-dimethyl-3,4-dihydroisochromen-1-one (2), two novel isocumarin type attractants. Based on these results, it is concluded that HSCCC is a viable separation method option for purifying insect attractants, while effectively maintaining the attracting activity of the isolates. This is the first attempt to apply counter-current chromatography technique to separate attractants from Chinese cockroach.
Keywords: attractants; counter-current chromatograph; ground beetles; Eupolyphaga sinensis Walker attractants; counter-current chromatograph; ground beetles; Eupolyphaga sinensis Walker
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Jiang, W.; Wu, X.; Wu, B. Isolation and Identification of Two Novel Attractant Compounds from Chinese Cockroach (Eupolyphaga sinensis Walker) by Combination of HSCCC, NMR and CD Techniques. Molecules 2013, 18, 11299-11310.

AMA Style

Jiang W, Wu X, Wu B. Isolation and Identification of Two Novel Attractant Compounds from Chinese Cockroach (Eupolyphaga sinensis Walker) by Combination of HSCCC, NMR and CD Techniques. Molecules. 2013; 18(9):11299-11310.

Chicago/Turabian Style

Jiang, Wei; Wu, Xiaodan; Wu, Bin. 2013. "Isolation and Identification of Two Novel Attractant Compounds from Chinese Cockroach (Eupolyphaga sinensis Walker) by Combination of HSCCC, NMR and CD Techniques." Molecules 18, no. 9: 11299-11310.


Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert