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Molecules 2013, 18(9), 11022-11032; doi:10.3390/molecules180911022
Article

The Inhibitory Effects of Phenolic and Terpenoid Compounds from Baccharis trimera in Siha Cells: Differences in Their Activity and Mechanism of Action

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1 Laboratório de Fitoquímica e Síntese Orgânica (LAFIS), Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil 2 Laboratório de Análises Bioquímicas e Citológicas (LABC), Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil 3 Instituto Federal de Educação, Ciência e Tecnologia, Porto Alegre 90030-041, RS, Brazil 4 Laboratório de Sinalização e Plasticidade Celular, Departamento de Biofísica, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, RS, Brazil
* Author to whom correspondence should be addressed.
Received: 25 July 2013 / Revised: 3 September 2013 / Accepted: 3 September 2013 / Published: 9 September 2013
(This article belongs to the Section Natural Products)
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Abstract

Baccharis trimera is used in folk medicine as a tea for digestive and liver diseases. It possesses anti-inflammatory and antioxidant properties that are related to the presence of phenolic compounds. The aim of this work was to investigate the anti-proliferative properties of phenolic (PHE) and terpenoid (SAP) compounds from B. trimera on human cervical cancer. The treatment of SiHa cells with PHE for 24 h suppressed colony formation in a dose-dependent manner, inhibited proliferation and inhibited cell motility. Although SAP inhibited the proliferation of SiHa cells in a dose-dependent manner, it increased colony formation and did not inhibit cell motility. PHE and SAP also promoted a significant increase in lactate dehydrogenase levels in the culture medium in a dose-dependent manner, indicating a loss of cell membrane integrity. Moreover, PHE promoted necrotic cell death, whereas SAP induced apoptosis. These compounds are new anticancer prototypes due their significant anticancer activity demonstrated herein.
Keywords: phenolic compounds; terpenoids; cervical cancer; necrosis; apoptosis; Baccharis trimera phenolic compounds; terpenoids; cervical cancer; necrosis; apoptosis; Baccharis trimera
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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de Oliveira, C.B.; Comunello, L.N.; Maciel, É.S.; Giubel, S.R.; Bruno, A.N.; Chiela, E.C.F.; Lenz, G.; Gnoatto, S.C.B.; Buffon, A.; Gosmann, G. The Inhibitory Effects of Phenolic and Terpenoid Compounds from Baccharis trimera in Siha Cells: Differences in Their Activity and Mechanism of Action. Molecules 2013, 18, 11022-11032.

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