Molecules 2013, 18(9), 11022-11032; doi:10.3390/molecules180911022
Article

The Inhibitory Effects of Phenolic and Terpenoid Compounds from Baccharis trimera in Siha Cells: Differences in Their Activity and Mechanism of Action

1 Laboratório de Fitoquímica e Síntese Orgânica (LAFIS), Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil 2 Laboratório de Análises Bioquímicas e Citológicas (LABC), Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 90610-000, RS, Brazil 3 Instituto Federal de Educação, Ciência e Tecnologia, Porto Alegre 90030-041, RS, Brazil 4 Laboratório de Sinalização e Plasticidade Celular, Departamento de Biofísica, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, RS, Brazil
* Author to whom correspondence should be addressed.
Received: 25 July 2013; in revised form: 3 September 2013 / Accepted: 3 September 2013 / Published: 9 September 2013
(This article belongs to the Section Natural Products)
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Abstract: Baccharis trimera is used in folk medicine as a tea for digestive and liver diseases. It possesses anti-inflammatory and antioxidant properties that are related to the presence of phenolic compounds. The aim of this work was to investigate the anti-proliferative properties of phenolic (PHE) and terpenoid (SAP) compounds from B. trimera on human cervical cancer. The treatment of SiHa cells with PHE for 24 h suppressed colony formation in a dose-dependent manner, inhibited proliferation and inhibited cell motility. Although SAP inhibited the proliferation of SiHa cells in a dose-dependent manner, it increased colony formation and did not inhibit cell motility. PHE and SAP also promoted a significant increase in lactate dehydrogenase levels in the culture medium in a dose-dependent manner, indicating a loss of cell membrane integrity. Moreover, PHE promoted necrotic cell death, whereas SAP induced apoptosis. These compounds are new anticancer prototypes due their significant anticancer activity demonstrated herein.
Keywords: phenolic compounds; terpenoids; cervical cancer; necrosis; apoptosis; Baccharis trimera

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MDPI and ACS Style

de Oliveira, C.B.; Comunello, L.N.; Maciel, É.S.; Giubel, S.R.; Bruno, A.N.; Chiela, E.C.F.; Lenz, G.; Gnoatto, S.C.B.; Buffon, A.; Gosmann, G. The Inhibitory Effects of Phenolic and Terpenoid Compounds from Baccharis trimera in Siha Cells: Differences in Their Activity and Mechanism of Action. Molecules 2013, 18, 11022-11032.

AMA Style

de Oliveira CB, Comunello LN, Maciel ÉS, Giubel SR, Bruno AN, Chiela ECF, Lenz G, Gnoatto SCB, Buffon A, Gosmann G. The Inhibitory Effects of Phenolic and Terpenoid Compounds from Baccharis trimera in Siha Cells: Differences in Their Activity and Mechanism of Action. Molecules. 2013; 18(9):11022-11032.

Chicago/Turabian Style

de Oliveira, Cristiane B.; Comunello, Lucimara N.; Maciel, Érica S.; Giubel, Scheron R.; Bruno, Alessandra N.; Chiela, Eduardo C.F.; Lenz, Guido; Gnoatto, Simone C.B.; Buffon, Andréia; Gosmann, Grace. 2013. "The Inhibitory Effects of Phenolic and Terpenoid Compounds from Baccharis trimera in Siha Cells: Differences in Their Activity and Mechanism of Action." Molecules 18, no. 9: 11022-11032.

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