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Molecules 2013, 18(8), 9919-9932; doi:10.3390/molecules18089919
Article

Chemistry and Antiviral Activity of Arrabidaea pulchra (Bignoniaceae)

1,2, 3, 1, 4 and 1,*
1 Faculdade de Farmácia, Departamento de Produtos Farmacêuticos, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, CEP 31.270-901, Belo Horizonte, MG, Brazil 2 Escola de Farmácia, Departamento de Farmácia, Universidade Federal de Ouro Preto, Rua Costa Sena, 171, CEP 35.400-000, Ouro Preto, MG, Brazil 3 Departamento de Microbiologia, ICB, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, CEP 31.270-901, Belo Horizonte, MG, Brazil 4 Departamento de Química, ICEX, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, CEP 31.270-901, Belo Horizonte, MG, Brazil
* Author to whom correspondence should be addressed.
Received: 26 April 2013 / Revised: 22 July 2013 / Accepted: 24 July 2013 / Published: 16 August 2013
(This article belongs to the Section Metabolites)
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Abstract

The aim of the present work was to carry out a bioguided isolation of antiviral chemical constituents from an ethanol extract of leaves from Arrabidaea pulchra (Cham.) Sandwith (EEAPL) that had shown in vitro activity in a previous screening using DNA and RNA viruses. The activity of EEPAL was evaluated against the DNA viruses Human herpesvirus 1 (HSV-1) and Vaccinia virus Western Reserve (VACV-WR) as well as against the RNA viruses Murine encephalomyocarditis virus (EMCV), and Dengue virus 2 (DENV-2) by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Cytotoxicity was determined in LLCMK2 and Vero cells and the Selectivity Indexes (SI) were calculated. The most potent effect was observed against DENV-2 (EC50 46.8 ± 1.6 µg mL−1; SI 2.7). For HSV-1 and VACV-WR EC50 values > 200 µg mL−1 were determined, while no inhibition of the cytopathic effect was observed with EMCV. Bioguided fractionation of EEAPL by partition between immiscible solvents followed by chromatography over a Sephadex LH20 column afforded two arylpropanoid glycosides, verbascoside (AP 1) and caffeoylcalleryanin (AP 2), along with a terpenoid, ursolic acid (AP 3). AP 1 and AP 3 exhibited similar anti-DENV-2 profiles, with SI values of 3.8 and 3.1, respectively, while AP 2 was the most effective anti-DENV-2 constituent, with a SI of 20.0. Our results show that A. pulchra leaves ethanol extract (EEAPL) affords compounds with antiviral activity, mainly against DENV-2.
Keywords: antiviral activity; dengue virus; Arrabidaea pulchra; verbascoside; caffeoylcallerianin antiviral activity; dengue virus; Arrabidaea pulchra; verbascoside; caffeoylcallerianin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Brandão, G.C.; Kroon, E.G.; Souza, D.E.; Filho, J.D.S.; Oliveira, A.B. Chemistry and Antiviral Activity of Arrabidaea pulchra (Bignoniaceae). Molecules 2013, 18, 9919-9932.

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