Molecules 2013, 18(8), 9933-9948; doi:10.3390/molecules18089933
Article

Characterization and Bioactivity of Polysaccharides Obtained from Pine Cones of Pinus koraiensis by Graded Ethanol Precipitation

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Received: 8 June 2013; in revised form: 14 August 2013 / Accepted: 15 August 2013 / Published: 19 August 2013
(This article belongs to the Section Metabolites)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Pinus koraiensis polysaccharides (PKP) were extracted by hot water from P. koraiensis pine cones. Five polysaccharide fractions named PKP-A, PKP-B, PKP-C, PKP-D and PKP-E were successfully separated at final ethanol concentrations of 30%, 50%, 60%, 70% and 80%, respectively. HPLC, FT-IR, GC-MS and automatic amino-acid analysis were applied to investigate their chemical characteristics. Monosaccharide component analysis indicated that the five fractions were all composed of d-ribose, l-rhamnose, l-arabinose, d-xylose, d-mannose, d-glucose and d-galactose, but their molar ratios were quite different. HPLC results revealed that the polysaccharides precipitated by higher concentrations of ethanol solution had lower molecular masses. Moreover, the antioxidant activities of the five fractions were studied on the basis of hydroxyl radical and ABTS radical scavenging tests. The five graded polysaccharide fractions exhibited good inhibitory power, and MTT tests in vitro showed the IC50 of PKP-A and PKP-E were 1,072.5 and 2,070.0 μg·mL−1, respectively. These results demonstrated that the PKP could be a potential source of natural antioxidants or dietary supplements.
Keywords: pine cones; Pinus koraiensis; polysaccharides; graded ethanol precipitation; chemical characterization; bioactivity
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MDPI and ACS Style

Zou, P.; Yang, X.; Huang, W.-W.; Zhao, H.-T.; Wang, J.; Xu, R.-B.; Hu, X.-L.; Shen, S.-Y.; Qin, D. Characterization and Bioactivity of Polysaccharides Obtained from Pine Cones of Pinus koraiensis by Graded Ethanol Precipitation. Molecules 2013, 18, 9933-9948.

AMA Style

Zou P, Yang X, Huang W-W, Zhao H-T, Wang J, Xu R-B, Hu X-L, Shen S-Y, Qin D. Characterization and Bioactivity of Polysaccharides Obtained from Pine Cones of Pinus koraiensis by Graded Ethanol Precipitation. Molecules. 2013; 18(8):9933-9948.

Chicago/Turabian Style

Zou, Pan; Yang, Xin; Huang, Wei-Wei; Zhao, Hai-Tian; Wang, Jing; Xu, Ren-Bo; Hu, Xing-Long; Shen, Si-Yan; Qin, Di. 2013. "Characterization and Bioactivity of Polysaccharides Obtained from Pine Cones of Pinus koraiensis by Graded Ethanol Precipitation." Molecules 18, no. 8: 9933-9948.

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