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Molecules 2013, 18(8), 9755-9769; doi:10.3390/molecules18089755
Article

Characterization of Nucleosides and Nucleobases in Natural Cordyceps by HILIC–ESI/TOF/MS and HILIC–ESI/MS

1, 1,2, 3, 3, 3 and 2,*
1 Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, Shangdong, China 2 National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China 3 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
* Author to whom correspondence should be addressed.
Received: 2 July 2013 / Accepted: 8 August 2013 / Published: 15 August 2013
Download PDF [265 KB, 18 June 2014; original version 18 June 2014]

Abstract

A method combining hydrophilic interaction chromatography (HILIC) and electrospray ionization mass spectrometry (ESI-MS) was developed for the characterization and determination of natural Cordyceps. Separation was achieved on a Waters Xbridge Amide column with gradient elution. Identification of 15 target nucleosides and nucleobases was based on retention time, UV spectra and mass measurements of the protonated molecules ([M+H]+) and main fragment ions (ESI-TOF/MS). Eight non-target compounds were tentatively identified by ESI-TOF/MS. The 15 target compounds were quantified by HILIC-ESI-MS/MS using time-programmed selective ion monitoring or multiple reaction monitoring in positive-ion mode under optimized mass conditions. This technique showed good linearity, repeatability and recovery. This approach was also successfully implemented in the analysis of nucleosides and nucleobases in 12 batches of natural Cordyceps samples that were collected from different regions in China. The developed HILIC-ESI-MS method exhibited clear advantages in identifying and determining highly polar bioactive components in Cordyceps, as well as their quality control.
Keywords: Cordyceps; nucleosides and nucleobases; hydrophilic interaction chromatography; electrospray ionization time-of-flight mass spectrometry; electrospray ionization tandem mass spectrometry Cordyceps; nucleosides and nucleobases; hydrophilic interaction chromatography; electrospray ionization time-of-flight mass spectrometry; electrospray ionization tandem mass spectrometry
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Zhao, H.-Q.; Wang, X.; Li, H.-M.; Yang, B.; Yang, H.-J.; Huang, L. Characterization of Nucleosides and Nucleobases in Natural Cordyceps by HILIC–ESI/TOF/MS and HILIC–ESI/MS. Molecules 2013, 18, 9755-9769.

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