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Molecules 2013, 18(8), 9717-9726; doi:10.3390/molecules18089717
Article

Highly Efficient Biotransformation of Polydatin to Resveratrol by Snailase Hydrolysis Using Response Surface Methodology Optimization

1,2, 3, 1, 1,* , 1 and 3,*
Received: 5 June 2013 / Revised: 15 July 2013 / Accepted: 16 July 2013 / Published: 13 August 2013
(This article belongs to the Section Metabolites)
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Abstract

Resveratrol (RV), a dietary antioxidant polyphenolic compound found in grapes and red wine, exerts a wide variety of pharmacological activities. However, lower content in plants compared with polydatin (PD, the glucoside of RV) limits its application in the food and pharmaceutical industries. In this paper, we carried out efficient biotransformation of PD to RV with 100% conversion yield by snailase hydrolysis. Moreover, response surface methodology (RSM) was used to optimize the effects of the reaction temperature, enzyme load, and reaction time on the conversion process. Validation of the RSM model was verified by the good agreement between the experimental and the predicted RV yield values. The optimum preparation conditions were as follows: temperature of 62.0 °C, enzyme load of 6.6%, and reaction time of 96 min. The proposed method may be highly applicable for the enzymatic preparation of RV for medicinal purposes.
Keywords: snailase hydrolysis; polydatin; resveratrol; response surface methodology snailase hydrolysis; polydatin; resveratrol; response surface methodology
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Wang, Z.; Zhao, L.-C.; Li, W.; Zhang, L.-X.; Zhang, J.; Liang, J. Highly Efficient Biotransformation of Polydatin to Resveratrol by Snailase Hydrolysis Using Response Surface Methodology Optimization. Molecules 2013, 18, 9717-9726.

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