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Highly Efficient Biotransformation of Polydatin to Resveratrol by Snailase Hydrolysis Using Response Surface Methodology Optimization
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China
College of Vocation and technology, Changchun University of Science and Technology, Changchun 130600, China
The Affiliated Ruikang Hospital, Guangxi University of Chinese Medicine, Nanning 530011, China
* Authors to whom correspondence should be addressed.
Received: 5 June 2013; in revised form: 15 July 2013 / Accepted: 16 July 2013 / Published: 13 August 2013
Abstract: Resveratrol (RV), a dietary antioxidant polyphenolic compound found in grapes and red wine, exerts a wide variety of pharmacological activities. However, lower content in plants compared with polydatin (PD, the glucoside of RV) limits its application in the food and pharmaceutical industries. In this paper, we carried out efficient biotransformation of PD to RV with 100% conversion yield by snailase hydrolysis. Moreover, response surface methodology (RSM) was used to optimize the effects of the reaction temperature, enzyme load, and reaction time on the conversion process. Validation of the RSM model was verified by the good agreement between the experimental and the predicted RV yield values. The optimum preparation conditions were as follows: temperature of 62.0 °C, enzyme load of 6.6%, and reaction time of 96 min. The proposed method may be highly applicable for the enzymatic preparation of RV for medicinal purposes.
Keywords: snailase hydrolysis; polydatin; resveratrol; response surface methodology
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MDPI and ACS Style
Wang, Z.; Zhao, L.-C.; Li, W.; Zhang, L.-X.; Zhang, J.; Liang, J. Highly Efficient Biotransformation of Polydatin to Resveratrol by Snailase Hydrolysis Using Response Surface Methodology Optimization. Molecules 2013, 18, 9717-9726.
Wang Z, Zhao L-C, Li W, Zhang L-X, Zhang J, Liang J. Highly Efficient Biotransformation of Polydatin to Resveratrol by Snailase Hydrolysis Using Response Surface Methodology Optimization. Molecules. 2013; 18(8):9717-9726.
Wang, Zi; Zhao, Li-Chun; Li, Wei; Zhang, Lian-Xue; Zhang, Jing; Liang, Jian. 2013. "Highly Efficient Biotransformation of Polydatin to Resveratrol by Snailase Hydrolysis Using Response Surface Methodology Optimization." Molecules 18, no. 8: 9717-9726.