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Molecules 2013, 18(8), 9021-9033; doi:10.3390/molecules18089021
Article

Solid-, Solution-, and Gas-state NMR Monitoring of 13C-Cellulose Degradation in an Anaerobic Microbial Ecosystem

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Received: 27 June 2013; in revised form: 10 July 2013 / Accepted: 19 July 2013 / Published: 29 July 2013
(This article belongs to the Special Issue NMR of Proteins and Small Biomolecules)
Download PDF [1616 KB, uploaded 18 June 2014]
Abstract: Anaerobic digestion of biomacromolecules in various microbial ecosystems is influenced by the variations in types, qualities, and quantities of chemical components. Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool for characterizing the degradation of solids to gases in anaerobic digestion processes. Here we describe a characterization strategy using NMR spectroscopy for targeting the input solid insoluble biomass, catabolized soluble metabolites, and produced gases. 13C-labeled cellulose produced by Gluconacetobacter xylinus was added as a substrate to stirred tank reactors and gradually degraded for 120 h. The time-course variations in structural heterogeneity of cellulose catabolism were determined using solid-state NMR, and soluble metabolites produced by cellulose degradation were monitored using solution-state NMR. In particular, cooperative changes between the solid NMR signal and 13C-13C/13C-12C isotopomers in the microbial degradation of 13C-cellulose were revealed by a correlation heat map. The triple phase NMR measurements demonstrated that cellulose was anaerobically degraded, fermented, and converted to methane gas from organic acids such as acetic acid and butyric acid.
Keywords: solid-state NMR; solution-state NMR; gas-state NMR; anaerobic digestion; bacterial cellulose solid-state NMR; solution-state NMR; gas-state NMR; anaerobic digestion; bacterial cellulose
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yamazawa, A.; Iikura, T.; Shino, A.; Date, Y.; Kikuchi, J. Solid-, Solution-, and Gas-state NMR Monitoring of 13C-Cellulose Degradation in an Anaerobic Microbial Ecosystem. Molecules 2013, 18, 9021-9033.

AMA Style

Yamazawa A, Iikura T, Shino A, Date Y, Kikuchi J. Solid-, Solution-, and Gas-state NMR Monitoring of 13C-Cellulose Degradation in an Anaerobic Microbial Ecosystem. Molecules. 2013; 18(8):9021-9033.

Chicago/Turabian Style

Yamazawa, Akira; Iikura, Tomohiro; Shino, Amiu; Date, Yasuhiro; Kikuchi, Jun. 2013. "Solid-, Solution-, and Gas-state NMR Monitoring of 13C-Cellulose Degradation in an Anaerobic Microbial Ecosystem." Molecules 18, no. 8: 9021-9033.


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