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Molecules 2013, 18(7), 8181-8199; doi:10.3390/molecules18078181

Purification of Ustiloxins A and B from Rice False Smut Balls by Macroporous Resins

MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
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Received: 3 June 2013 / Revised: 26 June 2013 / Accepted: 8 July 2013 / Published: 11 July 2013
(This article belongs to the Section Natural Products)
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Abstract

Ustiloxins are cyclopeptide mycotoxins produced by Villosiclava virens, the pathogenic fungus of rice false smut disease. Both resins SP207 and SP700 were screened to show the best adsorption and desorption properties for ustiloxins A and B among 20 commercial macroporous resins. Dynamic adsorption and desorption tests were carried out to optimize the process parameters. The optimal conditions for adsorption of resin SP207 were a processing volume as 32 bed volumes (BV), pH value of 4, and flow rate of 2 BV/h; and those for desorption of resin SP207 were a 40:60 (v/v) ratio of ethanol to water, an eluent volume of 4 BV, pH value of 4 and a flow rate of 3 BV/h. The optimal conditions for adsorption of resin SP700 were a processing volume of 26 BV, pH value as 4, flow rate of 2 BV/h; and those for desorption of resin SP700 were a 30:70 (v/v) ratio of ethanol to water solution as eluent, volume of 4 BV, pH value as 4 and flow rate of 2 BV/h. Under the optimal conditions; the purities of ustiloxins A and B obtained with resin SP207 increased 23.06-fold and 19.78-fold, respectively; and their recoveries were 96.67% and 81.25%; respectively. Similarly; the purities of ustiloxins A and B obtained with resin SP700 increased 14.75-fold and 15.33-fold and their recoveries were 93.65% and 88.64%; respectively. The results show that adsorption and desorption on SP207 and SP700 resins are effective strategies for purifying ustiloxins A and B. The developed methods are beneficial for large-scale preparation and purification of ustiloxins A and B from rice false smut balls.
Keywords: ustiloxins A and B; rice false smut balls; Villosiclava virens; macroporous resin; adsorption and desorption; purification ustiloxins A and B; rice false smut balls; Villosiclava virens; macroporous resin; adsorption and desorption; purification
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Shan, T.; Sun, W.; Wang, X.; Fu, X.; Sun, W.; Zhou, L. Purification of Ustiloxins A and B from Rice False Smut Balls by Macroporous Resins. Molecules 2013, 18, 8181-8199.

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