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Molecules 2013, 18(7), 7460-7472; doi:10.3390/molecules18077460
Article

Study on the Potential Toxicity of a Thymoquinone-Rich Fraction Nanoemulsion in Sprague Dawley Rats

1,2, 1, 3 and 1,2,*
Received: 27 May 2013; in revised form: 18 June 2013 / Accepted: 19 June 2013 / Published: 26 June 2013
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Abstract: Toxicological studies constitute an essential part of the effort in developing an herbal medicine into a drug product. A newly developed thymoquinone-rich fraction nanoemulsion (TQRFNE) has been prepared using a high pressure homogenizer. The purpose of this study was to investigate the potential acute toxicity of this nanoemulsion in Sprague Dawley rats. The acute toxicity studies were conducted as per the OECD guidelines 425, allowing for the use of test dose limit of 20 mL TQRFNE (containing 44.5 mg TQ)/kg. TQRFNE and distilled water (DW) as a control were administered orally to both sexes of rats on Day 0 and observed for 14 days. All the animals appeared normal, and healthy throughout the study. There was no observed mortality or any signs of toxicity during the experimental period. The effects of the TQRFNE and DW groups on general behavior, body weight, food and water consumption, relative organ weight, hematology, histopathology, and clinical biochemistry were measured. All the parameters measured were unaffected as compared to the control (DW) group. The administration of 20 mL TQRFNE /kg was not toxic after an acute exposure.
Keywords: Sprague-Dawley rats; nanoemulsion; thymoquinone rich fraction; acute oral toxicity; histopathology; hematology Sprague-Dawley rats; nanoemulsion; thymoquinone rich fraction; acute oral toxicity; histopathology; hematology
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Tubesha, Z.; Imam, M.U.; Mahmud, R.; Ismail, M. Study on the Potential Toxicity of a Thymoquinone-Rich Fraction Nanoemulsion in Sprague Dawley Rats. Molecules 2013, 18, 7460-7472.

AMA Style

Tubesha Z, Imam MU, Mahmud R, Ismail M. Study on the Potential Toxicity of a Thymoquinone-Rich Fraction Nanoemulsion in Sprague Dawley Rats. Molecules. 2013; 18(7):7460-7472.

Chicago/Turabian Style

Tubesha, Zaki; Imam, Mustapha U.; Mahmud, Rozi; Ismail, Maznah. 2013. "Study on the Potential Toxicity of a Thymoquinone-Rich Fraction Nanoemulsion in Sprague Dawley Rats." Molecules 18, no. 7: 7460-7472.


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