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Molecules 2013, 18(6), 7179-7193; doi:10.3390/molecules18067179
Article

Immunomodulatory Effect of Stichopus japonicus Acid Mucopolysaccharide on Experimental Hepatocellular Carcinoma in Rats

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Received: 3 May 2013 / Revised: 17 May 2013 / Accepted: 9 June 2013 / Published: 19 June 2013
(This article belongs to the Section Metabolites)
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Abstract

Stichopus japonicus acid mucopolysaccharide (SJAMP) is an important biologically active compound that can be extracted from the body wall of the sea cucumber. The present study investigated the anti-tumor and immunomodulatory effects of SJAMP in an experimental hepatocellular carcinoma (HCC) model in rats. Three doses of SJAMP (17.5 mg/kg, 35 mg/kg, and 70 mg/kg administered 5 days/week via oral gavage) were given to rats with diethylnitrosamine (DEN)-induced HCC. SJAMP treatment significantly inhibited DEN-induced HCC by reducing both the number and mean volume of nodules, decreasing serum a-fetoprotein (AFP) levels and proliferating cell nuclear antigen (PCNA) expression in liver, and increasing p21 expression. Furthermore, SJAMP decreased the serum levels of ALT, AST, GGT and TNF-α and increased serum IL-2. SJAMP administration also improved indices of spleen and thymus function and improved both macrophage phagocytosis and NK cell-mediated tumoricidal activity. Moreover, CD3+ and CD4+ T lymphocyte levels recovered significantly and the CD4+/CD8+ T cell ratio normalized in a dose-dependent manner. In conclusion, SJAMP effectively inhibited the growth of HCC through the stimulation of immune organs and tissue proliferation, leading to the enhancement of cellular immunity pathways in rats.
Keywords: hepatocellular carcinoma; Stichopus japonicus acid mucopolysaccharide; macrophage; natural killer cell; T lymphocyte subsets; proliferating cell nuclear antigen; p21 expression hepatocellular carcinoma; Stichopus japonicus acid mucopolysaccharide; macrophage; natural killer cell; T lymphocyte subsets; proliferating cell nuclear antigen; p21 expression
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Song, Y.; Jin, S.-J.; Cui, L.-H.; Ji, X.-J.; Yang, F.-G. Immunomodulatory Effect of Stichopus japonicus Acid Mucopolysaccharide on Experimental Hepatocellular Carcinoma in Rats. Molecules 2013, 18, 7179-7193.

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