Chemical Composition of Aspidosperma ulei Markgr. and Antiplasmodial Activity of Selected Indole Alkaloids
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolated Substances from A. ulei
2.2. Analysis of Spectral and Physical Data for Isolated Compounds
2.3. In Vitro Inhibition of P. falciparum and Cytotoxicity
Nº | Name | IC50 ± SD | IC50 ± SD | Result |
µg/mL | μM | |||
3 | 3,4,5,6-tetradehydro-β-yohimbine | 14.0 ± 2.7 | 39.9 ± 7.7 | I |
4 | 19E-hunteracine | > 50.0 | > 176 | I |
11 | 20-epi-dasycarpidone | 4.5 ± 0.2 | 16.7 ± 0.7 | MA |
12 | 20(E)-nor-subincanadine E | 14.5 ± 2.8 | 54.3 ± 10.5 | I |
13 | 12-hydroxy-N-acetyl-21(N)-dehydroplumeran-18-oic acid | > 50.0 | > 135 | I |
DS | chloroquine diphosphate | 0.17 ± 0.1 | 0.33 ± 0.19 | A |
DS | quinine sulphate | 0.12 ± 0.05 | 0.30 ± 0.15 | A |
3. Experimental
3.1. General Procedures
3.2. Collection, Botanic Identification and Processing of Plant Materials
3.3. Preparation of Extracts of A. ulei and Isolation Procedures
Dry, powdered plant material | Dry plant extract | |||||
---|---|---|---|---|---|---|
Part | Mass extracted (kg) | Name | Yield (g) | % Yield | Description | |
Heartwood | 3.0 | HWEE | 52 | 1.7 | Yellow powder | |
Leaf | 1.0 | LEE | 98 | 9.8 | Green powder | |
Root bark | 3.0 | RBEE | 274 | 9.1 | Viscous residue | |
Root wood | 3.0 | RWEE | 122 | 4.1 | Viscous residue | |
Stem bark | 2.0 | SBEE | 173 | 8.7 | Viscous residue |
3.3.1. Isolation of Chemical Components from Leaf Extracts
3.3.2. Isolation of Chemical Components from Stem Bark Extracts
3.3.3. Acid-Base Fractionation of EtOH Extracts
3.3.3.1. Isolation of Chemical Components from Acidic Fractions
3.3.3.2. Isolation of Chemical Components from Basic Fractions
3.4. Spectrometric Data for Isolated Compounds
3.5. Biological activity of isolated compounds from Aspidosperma ulei
3.5.1. In Vitro Culture of Plasmodium Falciparum and in Vitro Antiplasmodial Assay
3.5.2. Cell Culture and Cytotoxicity Test Using the Alamar BlueTM Assay
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Dos Santos Torres, Z.E.; Silveira, E.R.; Rocha e Silva, L.F.; Lima, E.S.; De Vasconcellos, M.C.; De Andrade Uchoa, D.E.; Filho, R.B.; Pohlit, A.M. Chemical Composition of Aspidosperma ulei Markgr. and Antiplasmodial Activity of Selected Indole Alkaloids. Molecules 2013, 18, 6281-6297. https://doi.org/10.3390/molecules18066281
Dos Santos Torres ZE, Silveira ER, Rocha e Silva LF, Lima ES, De Vasconcellos MC, De Andrade Uchoa DE, Filho RB, Pohlit AM. Chemical Composition of Aspidosperma ulei Markgr. and Antiplasmodial Activity of Selected Indole Alkaloids. Molecules. 2013; 18(6):6281-6297. https://doi.org/10.3390/molecules18066281
Chicago/Turabian StyleDos Santos Torres, Zelina Estevam, Edilberto Rocha Silveira, Luiz Francisco Rocha e Silva, Emerson Silva Lima, Marne Carvalho De Vasconcellos, Daniel Esdras De Andrade Uchoa, Raimundo Braz Filho, and Adrian Martin Pohlit. 2013. "Chemical Composition of Aspidosperma ulei Markgr. and Antiplasmodial Activity of Selected Indole Alkaloids" Molecules 18, no. 6: 6281-6297. https://doi.org/10.3390/molecules18066281