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Anti-Infective Potential of Marine Invertebrates and Seaweeds from the Brazilian Coast
Laboratório de Produtos Naturais, Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina, CEP 88.040-900, Florianópolis, SC, Brasil
Laboratório de Ficologia, Departamento de Botânica, Universidade Federal de Santa Catarina, CEP 88.040-900, Florianópolis, SC, Brasil
Laboratório de Virologia Aplicada, Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina, CEP 88.040-900, Florianópolis, SC, Brasil
Laboratório de Protozoologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, CEP 88.040-900, Florianópolis, SC, Brasil
Programa de Pós-graduação em Ecologia, Universidade Federal do Rio Grande do Sul, CEP 91.501-970, Porto Alegre, RS, Brasil
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 28 March 2013; in revised form: 3 May 2013 / Accepted: 10 May 2013 / Published: 16 May 2013
Abstract: This manuscript describes the evaluation of anti-infective potential in vitro of organic extracts from nine sponges, one ascidian, two octocorals, one bryozoan, and 27 seaweed species collected along the Brazilian coast. Antimicrobial activity was tested against Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922) and Candida albicans (ATCC 10231) by the disk diffusion method. Antiprotozoal activity was evaluated against Leishmania braziliensis (MHOM/BR/96/LSC96-H3) promastigotes and Trypanosoma cruzi (MHOM/BR/00/Y) epimastigotes by MTT assay. Activity against intracellular amastigotes of T. cruzi and L. brasiliensis in murine macrophages was also evaluated. Antiviral activity was tested against Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the plaque number reduction assay (IC50). Cytotoxicity on VERO cells was evaluated by the MTT assay (CC50). The results were expressed as SI = CC50/IC50. The most promising antimicrobial results were obtained against S. aureus and C. albicans with Dragmacidon reticulatum. Among the seaweeds, only Osmundaria obtusiloba showed moderate activity against P. aeruginosa. Concerning antiprotozoal activity, Bugula neritina, Carijoa riseii, Dragmaxia anomala and Haliclona (Halichoclona) sp. showed the most interesting results, mainly against extracellular promastigote forms of L. braziliensis (66, 35.9, 97.2, and 43.6% inhibition, respectively). Moreover, six species of seaweeds Anadyomene saldanhae, Caulerpa cupressoides, Canistrocarpus cervicornis, Dictyota sp., Ochtodes secundiramea, and Padina sp. showed promising results against L. braziliensis (87.9, 51.7, 85.9, 93.3, 99.7, and 80.9% inhibition, respectively), and only Dictyota sp. was effective against T. cruzi (60.4% inhibition). Finally, the antiherpes activity was also evaluated, with Haliclona (Halichoclona) sp. and Petromica citrina showing the best results (SI = 11.9 and SI > 5, respectively). All the active extracts deserve special attention in further studies to chemically characterize the bioactive compounds, and to perform more refined biological assays.
Keywords: marine natural products; seaweeds; marine invertebrates; antileishmanial activity; antitrypanosomal activity; antimicrobial activity; anti-HSV-1 activity
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Bianco, É.M.; de Oliveira, S.Q.; Rigotto, C.; Tonini, M.L.; da Rosa Guimarães, T.; Bittencourt, F.; Gouvêa, L.P.; Aresi, C.; de Almeida, M.T.R.; Moritz, M.I.G.; Martins, C.D.L.; Scherner, F.; Carraro, J.L.; Horta, P.A.; Reginatto, F.H.; Steindel, M.; Simões, C.M.O.; Schenkel, E.P. Anti-Infective Potential of Marine Invertebrates and Seaweeds from the Brazilian Coast. Molecules 2013, 18, 5761-5778.
Bianco ÉM, de Oliveira SQ, Rigotto C, Tonini ML, da Rosa Guimarães T, Bittencourt F, Gouvêa LP, Aresi C, de Almeida MTR, Moritz MIG, Martins CDL, Scherner F, Carraro JL, Horta PA, Reginatto FH, Steindel M, Simões CMO, Schenkel EP. Anti-Infective Potential of Marine Invertebrates and Seaweeds from the Brazilian Coast. Molecules. 2013; 18(5):5761-5778.
Bianco, Éverson M.; de Oliveira, Simone Q.; Rigotto, Caroline; Tonini, Maiko L.; da Rosa Guimarães, Tatiana; Bittencourt, Francine; Gouvêa, Lidiane P.; Aresi, Cassandra; de Almeida, Maria T.R.; Moritz, Maria I.G.; Martins, Cintia D.L.; Scherner, Fernando; Carraro, João L.; Horta, Paulo A.; Reginatto, Flávio H.; Steindel, Mario; Simões, Cláudia M.O.; Schenkel, Eloir P. 2013. "Anti-Infective Potential of Marine Invertebrates and Seaweeds from the Brazilian Coast." Molecules 18, no. 5: 5761-5778.