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Molecules 2013, 18(5), 5472-5481; doi:10.3390/molecules18055472
Article

Enantioselective Esterification of Ibuprofen under Microwave Irradiation

1,2
, 2
, 1
, 2
, 2
, 2
, 2, 3,*  and 2,*
1 The Second Hospital of Jilin University, Changchun 130011, China 2 Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, College of Life Sciences, Jilin University, Changchun 130023, China 3 Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security, Shenyang University, Shenyang 110044, China
* Authors to whom correspondence should be addressed.
Received: 25 February 2013 / Revised: 3 May 2013 / Accepted: 6 May 2013 / Published: 13 May 2013
(This article belongs to the Section Organic Synthesis)
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Abstract

Enantioselective esterification of ibuprofen has been successfully carried out in an organic solvent catalyzed by recombinant APE 1547 (a thermophilic esterase from the archaeon Aeropyrum pernix K1). Here we used microwave irradiation (MW) as the mode of heating to improve the enzyme performance. Under the optimum conditions, the enzyme activity of APE 1547 was 4.16 μmol/mg/h and the enantioselectivity (E value) was 52.9. Compared with conventional heating, the enzyme activity and the enantioselectivity were increased about 21.9-fold and 1.4-fold, respectively. The results also indicated that APE 1547 can maintain 95% of its activity even after being used five times, suggesting that the enzyme is stable under low power MW conditions.
Keywords: ibuprofen; APE 1547; enzyme activity; microwave; enantioselectivity ibuprofen; APE 1547; enzyme activity; microwave; enantioselectivity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yang, Z.; Niu, X.; Fang, X.; Chen, G.; Zhang, H.; Yue, H.; Wang, L.; Zhao, D.; Wang, Z. Enantioselective Esterification of Ibuprofen under Microwave Irradiation. Molecules 2013, 18, 5472-5481.

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