Anti-Oxidative and Anti-Proliferative Activity on Human Prostate Cancer Cells Lines of the Phenolic Compounds from Corylopsis coreana Uyeki
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Identification
Fractions | DPPH radical scavenging activity SC50 (mg/mL) | Superoxide scavenging activity SC50 (mg/mL) |
---|---|---|
1 | >100 e | 59.02 ± 1.07 d |
2 | 43.16 ± 0.48 d | 23.64 ± 0.92 c |
3 | 20.26 ± 0.43 c | 13.35 ± 1.09 b |
4 | 11.22 ±0 24 b | 5.89 ± 0.91 a |
5 | 5.89 ± 0.03 a | 2.42 ± 0.73 a |
Ascorbic acid | 6.68 ± 0.11 a | - |
Allopurinol | - | 1.70 ± 0.92 a |
2.2. Anti-Oxidative Activity (DPPH Radical, Superoxide Scavenging Activity)
2.3. Cell Viability and Inhibition of Cancer Cell Proliferation
Compounds | DPPH radical scavenging activity SC50 (μM/mL) | Superoxide scavenging activity SC50 (μM/mL) |
---|---|---|
1 | 23.05 ± 0.92 d,e | 13.47 ± 0.05 b |
2 | 51.49 ± 0.77 g | 11.71 ± 0.36 b |
3 | 21.65 ± 0.72 d,e | 22.68 ± 0.35 d |
4 | 13.41 ± 0.10 c | 10.81 ± 0.07 c |
5 | >100 i | >100 e |
6 | 36.34 ± 0.93 f | 13.68 ± 0.74 b, c |
7 | 21.63 ± 0.70 e | 3.85 ± 0.03 a |
8 | 3.12 ± 0.05 a | 0.29 ± 0.69 a |
9 | 2.97 ± 0.04 a | 0.09 ± 0.02 a |
10 | 3.29 ± 0.26 a | 0.16 ± 0.02 a |
11 | 49.09 ± 0.92 g | 12.18 ± 0.14 b,c |
12 | 60.96 ± 0.70 h | 12.43 ± 0.24 b,c |
13 | 17.06 ± 0.15 d | 3.17 ± 0.78 a |
14 | 36.87 ± 0.44 f | 2.59 ± 0.75 a |
15 | 19.45 ± 0.05 d,e | 1.45 ± 0.24 a |
Ascorbic acid | 8.22 ± 0.64 b | - |
Allopurinol | - | 2.39±0.09 a |
Compounds | Anti-proliferative activity of LNCaP cancer cell line IC50 (μM/mL) | Anti-proliferative activity of Du-145 cancer cell line IC50 (μM/mL) |
---|---|---|
1 | >100 c | >100 a |
2 | >100 c | >100 a |
3 | >100 c | >100 a |
4 | >100 c | >100 a |
5 | >100 c | >100 a |
6 | >100 c | >100 a |
7 | >100 c | >100 a |
8 | 66.35 ± 1.44 b | >100 a |
9 | 43.08 ± 0.63 a | >100 a |
10 | 40.56 ± 1.41 a | >100 a |
11 | >100 c | >100 a |
12 | >100 c | >100 a |
13 | >100 c | >100 a |
14 | >100 c | >100 a |
15 | >100 c | >100 a |
3. Experimental
3.1. General Methods
3.2. Plant Material
3.3. Extraction and Isolation
3.4. Antioxidant Activity
3.4.1. Measurement of DPPH Radical Scavenging Activity
3.4.2. Xanthine Oxidase Superoxide Scavenging Activity
3.5. Anti-Proliferation and Cytotoxicity Assays
3.5.1. Cell Culture
3.5.2. Measurement of Cell Viability
3.5.3. Anti-Proliferation Assays
3.6. Statistical Analysis
4. Conclusions
Acknowledgments
References
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Kim, M.H.; Ha, S.Y.; Oh, M.H.; Kim, H.H.; Kim, S.R.; Lee, M.W. Anti-Oxidative and Anti-Proliferative Activity on Human Prostate Cancer Cells Lines of the Phenolic Compounds from Corylopsis coreana Uyeki. Molecules 2013, 18, 4876-4886. https://doi.org/10.3390/molecules18054876
Kim MH, Ha SY, Oh MH, Kim HH, Kim SR, Lee MW. Anti-Oxidative and Anti-Proliferative Activity on Human Prostate Cancer Cells Lines of the Phenolic Compounds from Corylopsis coreana Uyeki. Molecules. 2013; 18(5):4876-4886. https://doi.org/10.3390/molecules18054876
Chicago/Turabian StyleKim, Manh Heun, Sung Yi Ha, Myeong Hwan Oh, Han Hyuk Kim, So Ra Kim, and Min Won Lee. 2013. "Anti-Oxidative and Anti-Proliferative Activity on Human Prostate Cancer Cells Lines of the Phenolic Compounds from Corylopsis coreana Uyeki" Molecules 18, no. 5: 4876-4886. https://doi.org/10.3390/molecules18054876