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Molecules 2013, 18(3), 2978-2987; doi:10.3390/molecules18032978

The Identification and Characterization of Chitotriosidase Activity in Pancreatin from Porcine Pancreas

1
Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan
2
Department of Chemical Engineering and Graduate School of Biochemical Engineering, Ming Chi University of Technology, 84 Gung-Juan Road, Taishan, Taipei 24301, Taiwan
3
Department of Environment and Biotechnology, Refining & Manufacturing Research Institute, CPC Corporation, 217 Min-Sheng S. Rd, Chiayi 60051, Taiwan
*
Authors to whom correspondence should be addressed.
Received: 21 November 2012 / Revised: 17 December 2012 / Accepted: 17 February 2013 / Published: 4 March 2013
(This article belongs to the Section Natural Products)
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Abstract

The versatile oligosaccharide biopolymers, chitin and chitosan, are typically produced using enzymatic processes. However, these processes are usually costly because chitinases and chitosanases are available in limited quantities. Fortunately, a number of commercial enzymes can hydrolyze chitin and chitosan to produce long chain chitin or chitosan oligosaccharides. Here, a platform to screen for enzymes with chitinase and chitosanase activities using a single gel with glycol chitin or glycol chitosan as a substrate was applied. SDS-resistant chitinase and chitosanase activities were observed for pancreatin. Its chitotriosidase had an optimal hydrolysis pH of 4 in the substrate specificity assay. This activity was thermally unstable, but independent of 2-mercaptoethanol. This is the first time a chitotriosidase has been identified in the hog. This finding suggests that oligochitosaccharides can be mass-produced inexpensively using pancreatin.
Keywords: chitin; chitosan; oligosaccharides; pancreatin; chitotriosidase chitin; chitosan; oligosaccharides; pancreatin; chitotriosidase
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Shen, C.-R.; Liu, C.-L.; Lee, H.-P.; Chen, J.-K. The Identification and Characterization of Chitotriosidase Activity in Pancreatin from Porcine Pancreas. Molecules 2013, 18, 2978-2987.

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