Molecules 2013, 18(3), 2978-2987; doi:10.3390/molecules18032978
Article

The Identification and Characterization of Chitotriosidase Activity in Pancreatin from Porcine Pancreas

1email, 2,* email, 3email and 3,* email
Received: 21 November 2012; in revised form: 17 December 2012 / Accepted: 17 February 2013 / Published: 4 March 2013
(This article belongs to the Section Metabolites)
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Abstract: The versatile oligosaccharide biopolymers, chitin and chitosan, are typically produced using enzymatic processes. However, these processes are usually costly because chitinases and chitosanases are available in limited quantities. Fortunately, a number of commercial enzymes can hydrolyze chitin and chitosan to produce long chain chitin or chitosan oligosaccharides. Here, a platform to screen for enzymes with chitinase and chitosanase activities using a single gel with glycol chitin or glycol chitosan as a substrate was applied. SDS-resistant chitinase and chitosanase activities were observed for pancreatin. Its chitotriosidase had an optimal hydrolysis pH of 4 in the substrate specificity assay. This activity was thermally unstable, but independent of 2-mercaptoethanol. This is the first time a chitotriosidase has been identified in the hog. This finding suggests that oligochitosaccharides can be mass-produced inexpensively using pancreatin.
Keywords: chitin; chitosan; oligosaccharides; pancreatin; chitotriosidase
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Shen, C.-R.; Liu, C.-L.; Lee, H.-P.; Chen, J.-K. The Identification and Characterization of Chitotriosidase Activity in Pancreatin from Porcine Pancreas. Molecules 2013, 18, 2978-2987.

AMA Style

Shen C-R, Liu C-L, Lee H-P, Chen J-K. The Identification and Characterization of Chitotriosidase Activity in Pancreatin from Porcine Pancreas. Molecules. 2013; 18(3):2978-2987.

Chicago/Turabian Style

Shen, Chia-Rui; Liu, Chao-Lin; Lee, Hsiao-Ping; Chen, Jeen-Kuan. 2013. "The Identification and Characterization of Chitotriosidase Activity in Pancreatin from Porcine Pancreas." Molecules 18, no. 3: 2978-2987.


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