Next Article in Journal
Oleodaphnoic Acid and Coriaceol, Two New Natural Products from the Stem Bark of Wikstroemia coriacea
Previous Article in Journal
Rare Syringyl Acylated Flavonol Glycosides from the Aerial Parts of Leonurus japonicus Houtt.
Molecules 2013, 18(3), 2978-2987; doi:10.3390/molecules18032978

The Identification and Characterization of Chitotriosidase Activity in Pancreatin from Porcine Pancreas

2,* , 3
1 Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan 2 Department of Chemical Engineering and Graduate School of Biochemical Engineering, Ming Chi University of Technology, 84 Gung-Juan Road, Taishan, Taipei 24301, Taiwan 3 Department of Environment and Biotechnology, Refining & Manufacturing Research Institute, CPC Corporation, 217 Min-Sheng S. Rd, Chiayi 60051, Taiwan
* Authors to whom correspondence should be addressed.
Received: 21 November 2012 / Revised: 17 December 2012 / Accepted: 17 February 2013 / Published: 4 March 2013
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [472 KB, uploaded 18 June 2014]   |  


The versatile oligosaccharide biopolymers, chitin and chitosan, are typically produced using enzymatic processes. However, these processes are usually costly because chitinases and chitosanases are available in limited quantities. Fortunately, a number of commercial enzymes can hydrolyze chitin and chitosan to produce long chain chitin or chitosan oligosaccharides. Here, a platform to screen for enzymes with chitinase and chitosanase activities using a single gel with glycol chitin or glycol chitosan as a substrate was applied. SDS-resistant chitinase and chitosanase activities were observed for pancreatin. Its chitotriosidase had an optimal hydrolysis pH of 4 in the substrate specificity assay. This activity was thermally unstable, but independent of 2-mercaptoethanol. This is the first time a chitotriosidase has been identified in the hog. This finding suggests that oligochitosaccharides can be mass-produced inexpensively using pancreatin.
Keywords: chitin; chitosan; oligosaccharides; pancreatin; chitotriosidase chitin; chitosan; oligosaccharides; pancreatin; chitotriosidase
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Shen, C.-R.; Liu, C.-L.; Lee, H.-P.; Chen, J.-K. The Identification and Characterization of Chitotriosidase Activity in Pancreatin from Porcine Pancreas. Molecules 2013, 18, 2978-2987.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert