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Brazilin Inhibits Growth and Induces Apoptosis in Human Glioblastoma Cells
Herbal Crop Utilization Research Team, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-873, Korea
Department of Neurosurgery, Ajou University School of Medicine, Suwon, 443-721, Korea
Graduate School of Biotechnology and Department of Oriental Medicinal Materials & Processing, Kyung Hee University, Yongin 446-701, Korea
* Author to whom correspondence should be addressed.
Received: 26 November 2012; in revised form: 31 January 2013 / Accepted: 6 February 2013 / Published: 21 February 2013
Abstract: Brazilin, isolated from the methanol extract of the heart wood of Caesalpinia sappan, sensitizes cancer cells to apoptosis. Glioblastoma multiforme (GBM), which accounts for most cases of central nervous system malignancy, has a very poor prognosis and lacks effective therapeutic interventions. We, therefore, investigated the effects of different concentrations of and different periods of exposure to brazilin on cell proliferation and apoptosis in the glioma U87 cell line. Cell proliferation was investigated by MTT assays and growth curve analysis, apoptosis was assessed by FACS analysis and western blot studies. Brazilin showed dose-dependent inhibition of cell proliferation and induction of apoptosis in glioma cells. It also increased the ratio of cleaved poly-(ADP)-ribose polymerase and decreased the expression of caspase-3 and caspase-7.
Keywords: apoptosis; brazilin; Caesalpinia sappan; glioma U87
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Lee, D.-Y.; Lee, M.-K.; Kim, G.-S.; Noh, H.-J.; Lee, M.-H. Brazilin Inhibits Growth and Induces Apoptosis in Human Glioblastoma Cells. Molecules 2013, 18, 2449-2457.
Lee D-Y, Lee M-K, Kim G-S, Noh H-J, Lee M-H. Brazilin Inhibits Growth and Induces Apoptosis in Human Glioblastoma Cells. Molecules. 2013; 18(2):2449-2457.
Lee, Dae-Young; Lee, Mi-Kyoung; Kim, Geum-Soog; Noh, Hyung-Jun; Lee, Min-Ho. 2013. "Brazilin Inhibits Growth and Induces Apoptosis in Human Glioblastoma Cells." Molecules 18, no. 2: 2449-2457.