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• Coqueiro, A
• Regasini, LOctávio
• Skrzek, SCristina Gutkoski
• Queiroz, MMarçal Ferreira
• Silva, DHelena Siqueira
• da Silva Bolzani, V
• [+] on Google Scholar
• Coqueiro, A
• Regasini, LOctávio
• Skrzek, SCristina Gutkoski
• Queiroz, MMarçal Ferreira
• Silva, DHelena Siqueira
• da Silva Bolzani, V
• [+] on PubMed
• Coqueiro, A
• Regasini, LOctávio
• Skrzek, SCristina Gutkoski
• Queiroz, MMarçal Ferreira
• Silva, DHelena Siqueira
• da Silva Bolzani, V

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Molecules 2013, 18(2), 2376-2385; doi:10.3390/molecules18022376

Article

Free Radical Scavenging Activity of Kielmeyera variabilis (Clusiaceae)

Aline Coqueiro, Luis Octávio Regasini, Scheila Cristina Gutkoski Skrzek, Marcos Marçal Ferreira Queiroz, Dulce Helena Siqueira Silva and Vanderlan da Silva Bolzani*

Department of Organic Chemistry, Institute of Chemistry, São Paulo State University, Araraquara 14800-900, Brazil

* Author to whom correspondence should be addressed.

Received: 7 January 2013; in revised form: 1 February 2013 / Accepted: 1 February 2013 / Published: 19 February 2013

Download PDF Full-Text [237 KB, uploaded 19 February 2013 09:36 CET]

Abstract: As part of our ongoing research on antioxidant agents from Brazilian flora, we screened the free radical scavenging activity of two extracts and eight fractions of Kielmeyera variabilis (Clusiaceae) using DPPH^· (2,2-diphenyl-1-picrylhydrazyl-hydrate) and ABTS^·+ [2,2'-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid)] colorimetric assays. The ethyl acetate and n-butanol fractions of the leaves of K. variabilis displayed the strongest activity (IC₅₀ of 3.5 ± 0.3 and 4.4 ± 0.2 μg mL⁻¹ for DPPH^· and 6.6 ± 0.4 and 3.1 ± 0.1 μg mL⁻¹ for ABTS^·+, respectively). Chromatographic fractionation of the most potent fractions led to identification of three flavonols with previously described antioxidant activity, quercitrin (1), quercetin-3-O-b-glucoside (3), and quercetin-3-O-b-galactoside (4), and of one biflavone, podocarpusflavone A (2). This is the first time that the presence of these flavonoids in Kielmeyera variabilis has been reported.

Keywords: Kielmeyera variabilis; Clusiaceae; antioxidant; free radical scavenging activity; DPPH^·; ABTS^·+; flavonoid; flavonol

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