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Molecules 2013, 18(12), 15412-15421; doi:10.3390/molecules181215412
Communication

A Triple Staining Method for Accurate Cell Cycle Analysis Using Multiparameter Flow Cytometry

1
, 2,*  and 1,*
Received: 29 October 2013; in revised form: 5 November 2013 / Accepted: 6 December 2013 / Published: 11 December 2013
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Abstract: Cell cycle analysis is important for cancer research. We present herein a novel method for accurate cell cycle analysis. This method analyzes the cell cycle by multiparameter flow cytometry based on simultaneously labeling the cell nuclear DNA, RNA, and phosphorylated mitotic nuclei protein, using Hoechst 33342, pyronin Y, and MPM-2-Cy5, respectively, and our results demonstrated that this method could effectively divide the cell cycle into G0, G1, S, G2, and M phases. We further tested this method using the clinical anticancer agents crizotinib and taxol, and the results clearly illustrated that crizotinib and taxol arrested Jurkat cells in G0 and M phase, respectively. These results indicate that this method could be a very useful tool for cytokinetic and pharmacological research.
Keywords: cell cycle; pyronin Y; Hoechst 33342; MPM-2; flow cytometry; crizotinb; taxol cell cycle; pyronin Y; Hoechst 33342; MPM-2; flow cytometry; crizotinb; taxol
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Qiu, L.; Liu, M.; Pan, K. A Triple Staining Method for Accurate Cell Cycle Analysis Using Multiparameter Flow Cytometry. Molecules 2013, 18, 15412-15421.

AMA Style

Qiu L, Liu M, Pan K. A Triple Staining Method for Accurate Cell Cycle Analysis Using Multiparameter Flow Cytometry. Molecules. 2013; 18(12):15412-15421.

Chicago/Turabian Style

Qiu, Lin; Liu, Ming; Pan, Konglun. 2013. "A Triple Staining Method for Accurate Cell Cycle Analysis Using Multiparameter Flow Cytometry." Molecules 18, no. 12: 15412-15421.



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