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A Triple Staining Method for Accurate Cell Cycle Analysis Using Multiparameter Flow Cytometry
Institute for Nutritional Sciences, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
* Authors to whom correspondence should be addressed.
Received: 29 October 2013; in revised form: 5 November 2013 / Accepted: 6 December 2013 / Published: 11 December 2013
Abstract: Cell cycle analysis is important for cancer research. We present herein a novel method for accurate cell cycle analysis. This method analyzes the cell cycle by multiparameter flow cytometry based on simultaneously labeling the cell nuclear DNA, RNA, and phosphorylated mitotic nuclei protein, using Hoechst 33342, pyronin Y, and MPM-2-Cy5, respectively, and our results demonstrated that this method could effectively divide the cell cycle into G0, G1, S, G2, and M phases. We further tested this method using the clinical anticancer agents crizotinib and taxol, and the results clearly illustrated that crizotinib and taxol arrested Jurkat cells in G0 and M phase, respectively. These results indicate that this method could be a very useful tool for cytokinetic and pharmacological research.
Keywords: cell cycle; pyronin Y; Hoechst 33342; MPM-2; flow cytometry; crizotinb; taxol
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Cite This Article
MDPI and ACS Style
Qiu, L.; Liu, M.; Pan, K. A Triple Staining Method for Accurate Cell Cycle Analysis Using Multiparameter Flow Cytometry. Molecules 2013, 18, 15412-15421.
Qiu L, Liu M, Pan K. A Triple Staining Method for Accurate Cell Cycle Analysis Using Multiparameter Flow Cytometry. Molecules. 2013; 18(12):15412-15421.
Qiu, Lin; Liu, Ming; Pan, Konglun. 2013. "A Triple Staining Method for Accurate Cell Cycle Analysis Using Multiparameter Flow Cytometry." Molecules 18, no. 12: 15412-15421.