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Effect of HT042, Herbal Formula, on Longitudinal Bone Growth in Spontaneous Dwarf Rats
Department of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, Korea
Korea Institute of Science and Technology for Eastern Medicine (KISTEM), NeuMed Inc., Seoul 130-701, Korea
Department of Pharmacology, School of Medicine, Kyung Hee University, Seoul 130-701, Korea
* Author to whom correspondence should be addressed.
Received: 23 September 2013; in revised form: 15 October 2013 / Accepted: 16 October 2013 / Published: 28 October 2013
Abstract: HT042 is a new herbal prescription consisting of Astragalus membranaceus, Phlomis umbrosa and Eleutherococcus senticosus, which are used in Korean medicine to stimulate growth in children. We investigated the effects of HT042 on the body weight, longitudinal bone growth, and bone length in spontaneous dwarf rats (SDR). Male and female SDRs were divided into three groups: the control group (DW, 10 mL/kg/day), the recombinant human GH group (rhGH; 500 µg/kg/day), and the HT042 (100 mg/kg/day) group. Each group received the respective treatments for 10 days. Body weight was measured on day 10 of treatment. On day 8, tetracycline (20 mg/kg) was injected intraperitoneally into all individuals to form a fluorescent band on the newly synthesized bone. On day 10, femur and tibia lengths were measured using PIXImus. Body weight, longitudinal bone growth, and bone length were not affected in the HT042 group. In contrast, the rhGH group showed significantly increased body weight, longitudinal bone growth, and bone length. In conclusion, HT042 does not act through a GH-like effect to promote longitudinal bone growth.
Keywords: HT042; herbal extract; longitudinal bone growth; spontaneous dwarf rat
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Kim, J.Y.; Song, M.; Lee, D.; Song, J.; Park, S.W.; Park, J.; Park, S.; Choi, H.-Y.; Kim, H. Effect of HT042, Herbal Formula, on Longitudinal Bone Growth in Spontaneous Dwarf Rats. Molecules 2013, 18, 13271-13282.
Kim JY, Song M, Lee D, Song J, Park SW, Park J, Park S, Choi H-Y, Kim H. Effect of HT042, Herbal Formula, on Longitudinal Bone Growth in Spontaneous Dwarf Rats. Molecules. 2013; 18(11):13271-13282.
Kim, Ji Y.; Song, MiKyung; Lee, Donghun; Song, Jungbin; Park, Sang W.; Park, Juyeon; Park, Seungjoon; Choi, Ho-Young; Kim, Hocheol. 2013. "Effect of HT042, Herbal Formula, on Longitudinal Bone Growth in Spontaneous Dwarf Rats." Molecules 18, no. 11: 13271-13282.