Molecules 2013, 18(10), 12793-12808; doi:10.3390/molecules181012793
Article

Apoptosis Sensitization by Euphorbia Factor L1 in ABCB1-Mediated Multidrug Resistant K562/ADR Cells

Received: 9 September 2013; in revised form: 11 October 2013 / Accepted: 11 October 2013 / Published: 16 October 2013
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In this article, reversal activities of Euphorbia factor L1 (EFL1) against ABCB1-mediated multidrug resistance (MDR) and apoptosis sensitization in K562/ADR cells are reported. EFL1 decreased the IC50 values of anticancer agents in K562/ADR cells over-expressing ABCB1. However, EFL1 did not affect the IC50 values of anticancer agents in sensitive K562 cells. Additionally, EFL1 increased the intracellular accumulation of rhodamine 123 and doxorubicin in K562/ADR cells without affecting their accumulation in K562 cells. Furthermore, EFL1 sensitized the apoptosis triggered by vincristine in K562/ADR cells via mitochondrial pathway, as confirmed by Annexin V-FITC/PI detection and western blot. At the same time, EFL1 did not influence the apoptosis induced by vincristine in K562 cells. Western blot results showed that EFL1 did not affect the phosphorylation level of AKT and ERK in K562 and K562/ADR cells. Finally, EFL1 did not down-regulate protein expression of ABCB1.
Keywords: ABCB1; multidrug resistance; apoptosis; Euphorbia factor L1
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MDPI and ACS Style

Zhang, J.-Y.; Lin, M.-T.; Yi, T.; Tang, Y.-N.; Fan, L.-L.; He, X.-C.; Zhao, Z.-Z.; Chen, H.-B. Apoptosis Sensitization by Euphorbia Factor L1 in ABCB1-Mediated Multidrug Resistant K562/ADR Cells. Molecules 2013, 18, 12793-12808.

AMA Style

Zhang J-Y, Lin M-T, Yi T, Tang Y-N, Fan L-L, He X-C, Zhao Z-Z, Chen H-B. Apoptosis Sensitization by Euphorbia Factor L1 in ABCB1-Mediated Multidrug Resistant K562/ADR Cells. Molecules. 2013; 18(10):12793-12808.

Chicago/Turabian Style

Zhang, Jian-Ye; Lin, Min-Ting; Yi, Tao; Tang, Yi-Na; Fan, Lan-Lan; He, Xi-Cheng; Zhao, Zhong-Zhen; Chen, Hu-Biao. 2013. "Apoptosis Sensitization by Euphorbia Factor L1 in ABCB1-Mediated Multidrug Resistant K562/ADR Cells." Molecules 18, no. 10: 12793-12808.

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