Molecules 2013, 18(1), 845-858; doi:10.3390/molecules18010845
Article

Chamaejasmine Arrests Cell Cycle, Induces Apoptosis and Inhibits Nuclear NF-κB Translocation in the Human Breast Cancer Cell Line MDA-MB-231

Tingting Zhang 1,† email, Hongyang Yu 2,† email, Guanglu Dong 2 email, Li Cai 1,* email and Yuxian Bai 1,* email
1 Oncology Department of Internal Medicine, the Third Affiliated Hospital of Harbin Medical University, Harbin 150040, China 2 Department of Radiation Oncology, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China † These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 19 November 2012; in revised form: 4 January 2013 / Accepted: 5 January 2013 / Published: 11 January 2013
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Abstract: In this study, the anticancer activity of chamaejasmine was characterized in the human breast cancer cell line, MDA-MB-231. Cell viability and cell cycle distribution were determined by MTT assay and flow cytometry, respectively. Western blotting was performed to determine changes in levels of various proteins. Results showed that treatment with chamaejasmine (4–16 μM) inhibited cell proliferation, which correlated with G2/M phase arrest and apoptosis in MDA-MB-231 cells. Chamaejasmine treatment of MDA-MB-231 cells resulted in induction of WAF1/p21 and KIP1/p27, decrease in cyclins A and cyclins B1. Cyclin-dependent kinase (cdk) 2 and cdc2 was also decreased after chamaejasmine treatment. Moreover, inhibition of nuclear translocation, phosphorylation of NF-κB, activation of IKKα and IKKβ, inhibition of phosphorylation and degradation of IκBα were also detected in this work. Our findings suggested that chamaejasmine could be explored as a preventive and perhaps as a chemotherapeutic agent in the management of breast cancer.
Keywords: chamaejasmine; MDA-MB-231; MTT; flow cytometry; western blotting

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MDPI and ACS Style

Zhang, T.; Yu, H.; Dong, G.; Cai, L.; Bai, Y. Chamaejasmine Arrests Cell Cycle, Induces Apoptosis and Inhibits Nuclear NF-κB Translocation in the Human Breast Cancer Cell Line MDA-MB-231. Molecules 2013, 18, 845-858.

AMA Style

Zhang T, Yu H, Dong G, Cai L, Bai Y. Chamaejasmine Arrests Cell Cycle, Induces Apoptosis and Inhibits Nuclear NF-κB Translocation in the Human Breast Cancer Cell Line MDA-MB-231. Molecules. 2013; 18(1):845-858.

Chicago/Turabian Style

Zhang, Tingting; Yu, Hongyang; Dong, Guanglu; Cai, Li; Bai, Yuxian. 2013. "Chamaejasmine Arrests Cell Cycle, Induces Apoptosis and Inhibits Nuclear NF-κB Translocation in the Human Breast Cancer Cell Line MDA-MB-231." Molecules 18, no. 1: 845-858.

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