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Influence of Sample Processing on the Analysis of Carotenoids in Maize
Department of Chemistry, Lleida University, Alcalde Rovira Roure, 191, Lleida 25198, Spain
* Author to whom correspondence should be addressed.
Received: 4 September 2012; in revised form: 13 September 2012 / Accepted: 13 September 2012 / Published: 21 September 2012
(This article belongs to the Special Issue Carotenoids
Abstract: We performed a number of tests with the aim to develop an effective extraction method for the analysis of carotenoid content in maize seed. Mixtures of methanol–ethyl acetate (6:4, v/v) and methanol–tetrahydrofuran (1:1, v/v) were the most effective solvent systems for carotenoid extraction from maize endosperm under the conditions assayed. In addition, we also addressed sample preparation prior to the analysis of carotenoids by liquid chromatography (LC). The LC response of extracted carotenoids and standards in several solvents was evaluated and results were related to the degree of solubility of these pigments. Three key factors were found to be important when selecting a suitable injection solvent: compatibility between the mobile phase and injection solvent, carotenoid polarity and content in the matrix.
Keywords: carotenoids; extraction; solubility; injection solvent; liquid chromatography
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MDPI and ACS Style
Rivera, S.; Canela, R. Influence of Sample Processing on the Analysis of Carotenoids in Maize. Molecules 2012, 17, 11255-11268.
Rivera S, Canela R. Influence of Sample Processing on the Analysis of Carotenoids in Maize. Molecules. 2012; 17(9):11255-11268.
Rivera, Sol; Canela, Ramon. 2012. "Influence of Sample Processing on the Analysis of Carotenoids in Maize." Molecules 17, no. 9: 11255-11268.