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Physicochemical and Antioxidant Properties of Algerian Honey
Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia
Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh
Laboratory of Research on Local Animal Products, Ibn Khaldoun University, Tiaret 14000, Algeria
Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia
* Author to whom correspondence should be addressed.
Received: 15 July 2012; in revised form: 25 August 2012 / Accepted: 2 September 2012 / Published: 20 September 2012
Abstract: The aim of the present study was to characterize the physical, biochemical and antioxidant properties of Algerian honey samples (n = 4). Physical parameters, such as pH, moisture content, electrical conductivity (EC), total dissolved solids (TDS), color intensity, total sugar and sucrose content were measured. Several biochemical and antioxidant tests were performed to determine the antioxidant properties of the honey samples. The mean pH was 3.84 ± 0.01, and moisture the content was 13.21 ± 0.16%. The mean EC was 0.636 ± 0.001, and the mean TDS was 316.92 ± 0.92. The mean color was 120.58 ± 0.64 mm Pfund, and the mean 5-hydroxymethylfurfural (HMF) content was 21.49 mg/kg. The mean total sugar and reducing sugar contents were 67.03 ± 0.68 g/mL and 64.72 ± 0.52 g/g, respectively. The mean sucrose content was 2.29 ± 0.65%. High mean values of phenolic (459.83 ± 1.92 mg gallic acid/kg), flavonoid (54.23 ± 0.62 mg catechin/kg), ascorbic acid (159.70 ± 0.78 mg/kg), AEAC (278.15 ± 4.34 mg/kg), protein (3381.83 ± 6.19 mg/kg) and proline (2131.47 ± 0.90) contents, as well as DPPH (39.57% ± 4.18) and FRAP activities [337.77 ± 1.01 µM Fe (II)/100 g], were also detected, indicating that Algerian honey has a high antioxidant potential. Strong positive correlations were found between flavonoid, proline and ascorbic acid contents and color intensity with DPPH and FRAP values. Thus, the present study revealed that Algerian honey is a good source of antioxidants.
Keywords: Algerian honey; physicochemical properties; DPPH; FRAP; proline; ascorbic acid
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Khalil, M.I.; Moniruzzaman, M.; Boukraâ, L.; Benhanifia, M.; Islam, M.A.; Islam, M.N.; Sulaiman, S.A.; Gan, S.H. Physicochemical and Antioxidant Properties of Algerian Honey. Molecules 2012, 17, 11199-11215.
Khalil MI, Moniruzzaman M, Boukraâ L, Benhanifia M, Islam MA, Islam MN, Sulaiman SA, Gan SH. Physicochemical and Antioxidant Properties of Algerian Honey. Molecules. 2012; 17(9):11199-11215.
Khalil, Md. Ibrahim; Moniruzzaman, Mohammed; Boukraâ, Laïd; Benhanifia, Mokhtar; Islam, Md. Asiful; Islam, Md. Nazmul; Sulaiman, Siti Amrah; Gan, Siew Hua. 2012. "Physicochemical and Antioxidant Properties of Algerian Honey." Molecules 17, no. 9: 11199-11215.