Activity-Guided Isolation of Antioxidant Compounds from Rhizophora apiculata
Abstract
:1. Introduction
2. Results and Discussion
2.1. Antioxidant Activities of Crude Extract and Fractions
2.2. Total Phenolic Content
2.3. Separation of Compounds
2.4. Activity and Quantity Analysis of Isolated Compounds
Compound | IC50 (μg/mL) | ||
---|---|---|---|
DPPH | ABTS•+ | OH | |
Lyoniresinol-3α-O-β-arabinopyranoside | 2.06 | 1.64 | 5.83 |
Lyoniresinol-3α-O-β-rhamnoside | 2.64 | 2.09 | 9.07 |
Afzelechin-3-rahmnoside | 2.26 | 1.96 | 7.05 |
BHT | 55.20 | 9.63 | 45.58 |
Compound | Content (%) | ||
---|---|---|---|
twig | leaf | bark | |
Lyoniresinol-3α-O-β-arabinopyranoside | 0.047 | 0.012 | 0.068 |
Lyoniresinol-3α-O-β-rhamnoside | 0.059 | 0.016 | 0.066 |
Afzelechin-3-rahmnoside | 0.010 | 0.006 | 0.011 |
3. Experimental
3.1. Plant Material and Chemicals
3.2. Extraction and Chemical Isolation
3.3. Antioxidant Activity
3.3.1. DPPH free Radical Scavenging Assay Activity
3.3.2. ABTS Scavenging Activity
3.3.3. Hydroxyl Radical Scavenging Ability Assay
3.4. Total Phenolic Content
3.5. HPLC Analysis of Lignans
3.6. Statistical Analysis
4. Conclusions
Acknowledgments
- Sample Availability: Samples of the three compounds are available from the authors.
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Gao, M.; Xiao, H. Activity-Guided Isolation of Antioxidant Compounds from Rhizophora apiculata. Molecules 2012, 17, 10675-10682. https://doi.org/10.3390/molecules170910675
Gao M, Xiao H. Activity-Guided Isolation of Antioxidant Compounds from Rhizophora apiculata. Molecules. 2012; 17(9):10675-10682. https://doi.org/10.3390/molecules170910675
Chicago/Turabian StyleGao, Mingzhe, and Hongbin Xiao. 2012. "Activity-Guided Isolation of Antioxidant Compounds from Rhizophora apiculata" Molecules 17, no. 9: 10675-10682. https://doi.org/10.3390/molecules170910675