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Molecules 2012, 17(9), 10344-10354; doi:10.3390/molecules170910344
Article

Preparation of Dry Extract of Mikania glomerata Sprengel (Guaco) and Determination of Its Coumarin Levels by Spectrophotometry and HPLC-UV

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1 Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora 36036-900, MG, Brazil 2 Federal University of Viçosa, Viçosa 36570-900, MG, Brazil 3 Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora 36036-900, MG Brazil 4 Institute of Biology, Fluminense Federal University, Niterói 24220-900, RJ, Brazil
* Author to whom correspondence should be addressed.
Received: 11 June 2012 / Revised: 1 August 2012 / Accepted: 16 August 2012 / Published: 29 August 2012
(This article belongs to the Special Issue Coumarins and Xanthones)
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Abstract

Guaco (Mikania glomerata Sprengel) syrup is one of the most popular herbal medicines used to treat the symptoms of asthmatic bronchitis, cough and hoarseness. The coumarin 2H-1-benzopyran-2-one, is one of the major constituents of Guaco and contributes to its pharmacological effects. The pharmaceutical capsule form of dry extract of Guaco is recommended by the Brazilian Program of Medicinal Plants and Herbal Medicines and used in primary health care. In order to identify a new protocol to obtain the raw material for Guaco capsule production we evaluated two methods, including a freeze-drying process (lyophilization) and the spray-dryer technique, as well as the use of two adjuvants, Maltodextrins and Aerosil®, in different concentrations. The coumarin levels of the dried extracts were analyzed by UV-spectrophotometry and HPLC-UV/DAD. The adjuvant Aerosil® 8% showed better dry powder physical appearance. Lyophilization was observed to be the best process to obtain the dry extract of Guaco based on the measured coumarin levels.
Keywords: pharmacotechnique; freeze-dryer; lyophilization; coumarins pharmacotechnique; freeze-dryer; lyophilization; coumarins
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Soares e Silva, L.; Santos da Silva, L.; Brumano, L.; Stringheta, P.C.; Aparecida de Oliveira Pinto, M.; Moreira Dias, L.O.; de Sá Martins Muller, C.; Scio, E.; Fabri, R.L.; Castro, H.C.; da Penha Henriques do Amaral, M. Preparation of Dry Extract of Mikania glomerata Sprengel (Guaco) and Determination of Its Coumarin Levels by Spectrophotometry and HPLC-UV. Molecules 2012, 17, 10344-10354.

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