Molecules 2012, 17(9), 10322-10330; doi:10.3390/molecules170910322
Article

Antibacterial and Synergy of Berberines with Antibacterial Agents against Clinical Multi-Drug Resistant Isolates of Methicillin-Resistant Staphylococcus aureus (MRSA)

1 Research Center for Natural Medicines, Kunming General Hospital, PLA, Kunming 650032, China 2 Kunming Medical College, Kunming 650032, China 3 School of Basic Medical Sciences, Yunnan Traditional Chinese Medical College, Kunming 650500, China 4 Center for Infectious Diseases, Kunming General Hospital, PLA, Kunming 650032, China
* Author to whom correspondence should be addressed.
Received: 3 June 2012; in revised form: 19 August 2012 / Accepted: 21 August 2012 / Published: 29 August 2012
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Abstract: Antibacterial activity of berberine (Ber) and 8-acetonyl-dihydroberberine (A-Ber) alone and combined uses with antibacterial agents ampicillin (AMP), azithromycin (AZM), cefazolin (CFZ) and levofloxacin (LEV) was studied on 10 clinical isolates of SCCmec III type methicillin-resistant Staphylococcus aureus (MRSA). Susceptibility to each agent alone was tested using a broth microdilution method and the chequerboard and time-kill tests for the combined evaluations, respectively. The alone MICs/MBCs (mg/mL) ranges were 32–128/64–256 (Ber) and 32-128/128-512 (A-Ber). Significant synergies were observed for the Ber (A-Ber)/AZM and Ber (A-Ber)/LEV combinations against 90% of the tested MRSA strains, with fractional inhibitory concentration indices (FICIs) values ranged from 0.188 to 0.500. An additivity result was also observed for the Ber/AZM combination by time-kill curves. These results demonstrated for the first time that Ber and A-Ber enhanced the in vitro inhibitory efficacy of AZM and LEV to a same extent, which had potential for further investigation in combinatory therapeutic applications of patients infected with MRSA.
Keywords: anti-MRSA; synergy; 8-acetonyl-dihydroberberine; levofloxacin; FICI

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MDPI and ACS Style

Zuo, G.-Y.; Li, Y.; Han, J.; Wang, G.-C.; Zhang, Y.-L.; Bian, Z.-Q. Antibacterial and Synergy of Berberines with Antibacterial Agents against Clinical Multi-Drug Resistant Isolates of Methicillin-Resistant Staphylococcus aureus (MRSA). Molecules 2012, 17, 10322-10330.

AMA Style

Zuo G-Y, Li Y, Han J, Wang G-C, Zhang Y-L, Bian Z-Q. Antibacterial and Synergy of Berberines with Antibacterial Agents against Clinical Multi-Drug Resistant Isolates of Methicillin-Resistant Staphylococcus aureus (MRSA). Molecules. 2012; 17(9):10322-10330.

Chicago/Turabian Style

Zuo, Guo-Ying; Li, Yang; Han, Jun; Wang, Gen-Chun; Zhang, Yun-Ling; Bian, Zhong-Qi. 2012. "Antibacterial and Synergy of Berberines with Antibacterial Agents against Clinical Multi-Drug Resistant Isolates of Methicillin-Resistant Staphylococcus aureus (MRSA)." Molecules 17, no. 9: 10322-10330.

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