In Vitro and in Vivo Antitumor Effect of Trachylobane-360, a Diterpene from Xylopia langsdorffiana
Abstract
:1. Introduction
2. Results and Discussion
2.1. Hemolysis Assay
2.2. Antitumor Activity of Trachylobane-360
2.2.1. Effect of Trachylobane-360 on Tumor Cell in Culture
2.2.2. Effect of Trachylobane-360 on Tumor Growth in vivo
2.2.2.1. Toxicological Analyses
2.2.2.1.1. Effects of Trachylobane-360 on Organ and Body Weight
Drug | Dose (mg/kg) | Thymus (mg/g body weight) | Spleen (mg/g body weight) | Liver (mg/g body weight) | Kidney (mg/g body weight) | Initial weight (g) | Final weight (g) |
---|---|---|---|---|---|---|---|
Healthy mice | |||||||
(5% Tween-80) | - | 3.09 ± 0.30 | 5.21 ± 0.24 | 56.33 ± 0.49 | 12.23 ± 0.74 | 32.07 ± 0.79 | 32.38 ± 0.62 |
Control-S180 | |||||||
(5% Tween-80) | - | 4.36 ± 0.50 c | 6.85 ± 0.50 c | 63.30 ± 1.17 | 10.62 ± 0.13 | 31.43 ± 0.99 | 34.54 ± 0.90 |
5-FU | 25 | 2.13 ± 0.11 a | 5.24 ± 0.15 a | 57.52 ± 0.92 a | 10.67 ± 0.59 | 30.27 ± 0.49 | 30.35 ± 0.73 a |
Trachylobane-360 | 12.5 | 3.80 ± 0.31 b | 7.62 ± 0.37 b,c | 63.04 ± 1.60 | 11.39 ± 0.28 | 32.10 ± 0.68 | 33.77 ± 0.70 |
Trachylobane-360 | 25 | 3.41 ± 0.16 b | 7.59 ± 0.36 b,c | 58.75 ± 1.07 | 10.96 ± 0.56 | 29.73 ± 0.50 | 31.37 ± 0.53 a |
2.2.2.1.2. Effect of Trachylobane-360 on Biochemical Parameters
Drug | Dose (mg/kg) | AST (UI/L) | ALT (UI/L) | Urea (mg/dL) | Creatinine (mg/dL) |
---|---|---|---|---|---|
Healthy mice | |||||
(5% Tween-80) | - | 217.6 ± 26.2 | 122.6 ± 19.1 | 41.2 ± 2.2 | 0.28 ± 0,02 |
Control-S180 | |||||
(5% Tween-80) | - | 287.8 ± 20.2 | 71.8 ± 7.3 | 39.8 ± 4.4 | 0.28 ± 0.02 |
5-FU | 25 | 219.7 ± 18.4 | 63.8 ± 9.7 | 48.0 ± 3.5 | 0.32 ± 0.02 |
Trachylobane-360 | 12.5 | 275.0 ± 15.5 | 76.0 ± 10.4 | 48.8 ± 5.8 | 0.36 ± 0.06 |
Trachylobane-360 | 25 | 298.3 ± 13.5 | 109.0 ± 10.1 | 51.2 ± 4.7 | 0.40 ± 0.04 |
2.2.2.1.3. Effect of Trachylobane-360 on Hematological Parameters
Drug | Dose (mg/kg) | MCV ( fm3) | MCH (pg) | MCHC (g/dL) |
---|---|---|---|---|
Healthy mice | ||||
(5% Tween-80) | - | 43.40 ± 0.93 | 14.66 ± 0.34 | 33.70 ± 0.29 |
Control-S180 | ||||
(5% Tween-80) | - | 44.50 ± 1.52 | 14.60 ± 0.34 | 32.95 ± 0.53 |
5-FU | 25 | 43.67 ± 0.49 | 15.08 ± 0.23 | 34.45 ± 0.68 |
Trachylobane-360 | 12.5 | 42.83 ± 0.79 | 14.80 ± 0.30 | 34.63 ± 0.33 |
Trachylobane-360 | 25 | 43.33 ± 0.56 | 14.92 ± 0.13 | 34.60 ± 0.28 |
Drug | Dose (mg/kg) | RBC (106 mm−3) | Hemoglobin (g/dL) | Hematocrit (%) |
---|---|---|---|---|
Healthy mice | ||||
(5% Tween-80) | - | 9.87 ± 0.25 | 14.46 ± 0.27 | 42.94 ± 0.62 |
Control-S180 | ||||
(5% Tween-80) | - | 7.89 ± 0.36 c | 11.45 ± 0.73 c | 34.80 ± 2.35 |
5-FU | 25 | 8.60 ± 0.18 c | 12.95 ± 0.10 | 37.73 ± 0.93 |
Trachylobane-360 | 12.5 | 8.79 ± 0.18 c | 13.03 ± 0.36 | 37.63 ± 1.10 |
Trachylobane-360 | 25 | 9.67 ± 0.15 a,b | 14.28 ± 0.18 a | 40.55 ± 0.57 |
Drug | Dose(mg/kg) | Total leukocytes (103 mm−3) | Differential count of leukocytes (%) | |||
---|---|---|---|---|---|---|
Lymphocytes | Neutrophils | Monocytes | Eosinophils | |||
Healthy mice | ||||||
(5% Tween-80) | - | 7.08 ± 0.51 | 77.80 ± 3.73 | 18.40 ± 3.25 | 3.40 ± 0.51 | 0.40 ± 0.24 |
Control-S180 | ||||||
(5% Tween-80) | - | 13.34 ± 2.58 c | 44.00 ± 2.54 c | 50.50 ± 3.16 c | 4.33 ± 0.56 | 0.67 ± 0.33 |
5-FU | 25 | 3.95 ± 0.51 a | 77.17 ± 2.18 a | 18.50 ± 2.32a | 3.50 ± 0.62 | 0.50 ± 0.34 |
Trachylobane-360 | 12.5 | 11.15 ± 1.28 b | 51.83 ± 4.66 b,c | 41.17 ± 5.25 b,c | 6.00 ± 1.57 | 1.00 ± 0.45 |
Trachylobane-360 | 25 | 11.42 ± 0.92 b | 53.17 ± 5.08 b,c | 38.67 ± 4.56 b,c | 6.83 ± 1.11 | 0.50 ± 0.22 |
2.2.2.1.4. Histopathology Changes
3. Experimental
3.1. Drug
3.2. Animals and Tumor Cells
3.3. Hemolysis Assay
3.4. Antitumor Activity of Trachylobane-360
3.4.1. Determination of the Effect of Trachylobane-360 on Tumor Cells in Culture
3.4.2. Determination of the Effect of Trachylobane-360 on Tumor Growth in vivo
3.4.2.1. Toxicological Analyses
3.4.2.1.1. Determination of the Effect of Trachylobane-360 on Body and Organ Weight
3.4.2.1.2. Determination of the Effect of Trachylobane-360 on Biochemical Parameters
3.4.2.1.3. Determination of the Effect of Trachylobane-360 on Hematological Parameters
3.4.2.1.4. Histopathological Analyses
3.5. Statistical Analysis
4. Conclusions
Acknowledgments
References
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Pita, J.C.L.R.; Xavier, A.L.; Sousa, T.K.G.d.; Mangueira, V.M.; Tavares, J.F.; Júnior, R.J.d.O.; Veras, R.C.; Pessoa, H.d.L.F.; Silva, M.S.d.; Morelli, S.; et al. In Vitro and in Vivo Antitumor Effect of Trachylobane-360, a Diterpene from Xylopia langsdorffiana. Molecules 2012, 17, 9573-9589. https://doi.org/10.3390/molecules17089573
Pita JCLR, Xavier AL, Sousa TKGd, Mangueira VM, Tavares JF, Júnior RJdO, Veras RC, Pessoa HdLF, Silva MSd, Morelli S, et al. In Vitro and in Vivo Antitumor Effect of Trachylobane-360, a Diterpene from Xylopia langsdorffiana. Molecules. 2012; 17(8):9573-9589. https://doi.org/10.3390/molecules17089573
Chicago/Turabian StylePita, João Carlos Lima Rodrigues, Aline Lira Xavier, Tatyanna Kelvia Gomes de Sousa, Vivianne Mendes Mangueira, Josean Fechine Tavares, Robson José de Oliveira Júnior, Robson Cavalcante Veras, Hilzeth de Luna Freire Pessoa, Marcelo Sobral da Silva, Sandra Morelli, and et al. 2012. "In Vitro and in Vivo Antitumor Effect of Trachylobane-360, a Diterpene from Xylopia langsdorffiana" Molecules 17, no. 8: 9573-9589. https://doi.org/10.3390/molecules17089573