Molecules 2012, 17(8), 9462-9468; doi:10.3390/molecules17089462
Communication

Effect of Acute and Fractionated Irradiation on Hippocampal Neurogenesis

Received: 27 June 2012; in revised form: 27 July 2012 / Accepted: 6 August 2012 / Published: 8 August 2012
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Ionizing radiation has become an inevitable health concern emanating from natural sources like space travel and from artificial sources like medical therapies. In general, exposure to ionizing radiation such as γ-rays is one of the methods currently used to stress specific model systems. In this study, we elucidated the long-term effect of acute and fractionated irradiation on DCX-positive cells in hippocampal neurogenesis. Groups of two-month-old C57BL/6 female mice were exposed to whole-body irradiation at acute dose (5 Gy) or fractional doses (1 Gy × 5 times and 0.5 Gy × 10 times). Six months after exposure to γ-irradiation, the hippocampus was analyzed. Doublecortin (DCX) immunohistochemistry was used to measure changes of neurogenesis in the subgranular zone (SGZ) of the hippocampal dentate gyrus (DG). The number of DCX-positive cells was significantly decreased in all acute and fractionally irradiation groups. The long-term changes in DCX-positive cells triggered by radiation exposure showed a very different pattern to the short-term changes which tended to return to the control level in previous studies. Furthermore, the number of DCX-positive cells was relatively lower in the acute irradiation group than the fractional irradiation groups (approximately 3.6-fold), suggesting the biological change on hippocampal neurogenesis was more susceptible to being damaged by acute than fractional irradiation. These results suggest that the exposure to γ-irradiation as a long-term effect can trigger biological responses resulting in the inhibition of hippocampal neurogenesis.
Keywords: neurogenesis; ionizing radiation; immunohistochemistry; doublecortin; long-term effects
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MDPI and ACS Style

Park, M.-K.; Kim, S.; Jung, U.; Kim, I.; Kim, J.K.; Roh, C. Effect of Acute and Fractionated Irradiation on Hippocampal Neurogenesis. Molecules 2012, 17, 9462-9468.

AMA Style

Park M-K, Kim S, Jung U, Kim I, Kim JK, Roh C. Effect of Acute and Fractionated Irradiation on Hippocampal Neurogenesis. Molecules. 2012; 17(8):9462-9468.

Chicago/Turabian Style

Park, Min-Kyoung; Kim, Seolhwa; Jung, Uhee; Kim, Insub; Kim, Jin Kyu; Roh, Changhyun. 2012. "Effect of Acute and Fractionated Irradiation on Hippocampal Neurogenesis." Molecules 17, no. 8: 9462-9468.

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