Next Article in Journal
Insights on Cytochrome P450 Enzymes and Inhibitors Obtained Through QSAR Studies
Previous Article in Journal
Chemical Genetics: Budding Yeast as a Platform for Drug Discovery and Mapping of Genetic Pathways
Article Menu

Export Article

Open AccessArticle
Molecules 2012, 17(8), 9274-9282; doi:10.3390/molecules17089274

Extraction of Betulin, Trimyristin, Eugenol and Carnosic Acid Using Water-Organic Solvent Mixtures

Department of Chemistry, Pennsylvania State University-York, 1031 Edgecomb Avenue, York 17403, PA, USA
Received: 12 June 2012 / Revised: 26 July 2012 / Accepted: 27 July 2012 / Published: 3 August 2012
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [177 KB, uploaded 18 June 2014]   |  


A solvent system consisting of ethyl acetate, ethyl alcohol and water, in the volume ratio of 4.5:4.5:1, was developed and used to extract, at room temperature, betulin from white birch bark and antioxidants from spices (rosemary, thyme, sage, and oregano) and white oak chips. In addition, under reflux conditions, trimyristin was extracted from nutmeg using the same solvent system, and eugenol from olives was extracted using a mixture of salt water and ethyl acetate. The protocol demonstrates the use of water in organic solvents to extract natural products from plants. Measurement of the free-radical scavenging activity using by 2,2-diphenyl-1-picrylhydrazyl (DPPH) indicated that the extraction of plant material using ethyl acetate, ethyl alcohol and water (4.5:4.5:1, v/v/v) was exhaustive when carried out at room temperature for 96 h. View Full-Text
Keywords: toxic chemicals; liquid extraction; spices; antioxidants toxic chemicals; liquid extraction; spices; antioxidants

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Lugemwa, F.N. Extraction of Betulin, Trimyristin, Eugenol and Carnosic Acid Using Water-Organic Solvent Mixtures. Molecules 2012, 17, 9274-9282.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top