Molecules 2012, 17(8), 9274-9282; doi:10.3390/molecules17089274
Article

Extraction of Betulin, Trimyristin, Eugenol and Carnosic Acid Using Water-Organic Solvent Mixtures

Department of Chemistry, Pennsylvania State University-York, 1031 Edgecomb Avenue, York 17403, PA, USA
Received: 12 June 2012; in revised form: 26 July 2012 / Accepted: 27 July 2012 / Published: 3 August 2012
(This article belongs to the Section Natural Products)
PDF Full-text Download PDF Full-Text [177 KB, uploaded 3 August 2012 08:51 CEST]
Abstract: A solvent system consisting of ethyl acetate, ethyl alcohol and water, in the volume ratio of 4.5:4.5:1, was developed and used to extract, at room temperature, betulin from white birch bark and antioxidants from spices (rosemary, thyme, sage, and oregano) and white oak chips. In addition, under reflux conditions, trimyristin was extracted from nutmeg using the same solvent system, and eugenol from olives was extracted using a mixture of salt water and ethyl acetate. The protocol demonstrates the use of water in organic solvents to extract natural products from plants. Measurement of the free-radical scavenging activity using by 2,2-diphenyl-1-picrylhydrazyl (DPPH) indicated that the extraction of plant material using ethyl acetate, ethyl alcohol and water (4.5:4.5:1, v/v/v) was exhaustive when carried out at room temperature for 96 h.
Keywords: toxic chemicals; liquid extraction; spices; antioxidants

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Lugemwa, F.N. Extraction of Betulin, Trimyristin, Eugenol and Carnosic Acid Using Water-Organic Solvent Mixtures. Molecules 2012, 17, 9274-9282.

AMA Style

Lugemwa FN. Extraction of Betulin, Trimyristin, Eugenol and Carnosic Acid Using Water-Organic Solvent Mixtures. Molecules. 2012; 17(8):9274-9282.

Chicago/Turabian Style

Lugemwa, Fulgentius N. 2012. "Extraction of Betulin, Trimyristin, Eugenol and Carnosic Acid Using Water-Organic Solvent Mixtures." Molecules 17, no. 8: 9274-9282.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert