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Molecules 2012, 17(8), 8742-8752; doi:10.3390/molecules17088742
Article

Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from Catharanthus roseus Leaves

1,2,3,†, 1,2,3,†, 1,2,3, 1,2,3,4,* , 1,2,3, 1,2,3 and 1,2,3,*
1 Engineering Research Center of Forestry Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, China 2 Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China 3 State Engineering Laboratory of Bio-Resource Eco-Utilization, Harbin 150040, China 4 Zhejiang Hisun Pharmaceutical Co. Ltd., Taizhou 318000, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 15 May 2012 / Revised: 11 July 2012 / Accepted: 13 July 2012 / Published: 25 July 2012
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Abstract

In the present study, an improved method termed negative-pressure cavitation extraction (NPCE) followed by reverse phase high-performance liquid chromatography (RP-HPLC) was developed for the extraction and quantification of vindoline (VDL), catharanthine (CTR), vincristine (VCR) and vinblastine (VLB) from Catharanthus roseus leaves. The optimized method employed 60-mesh particles, 80% ethanol, a negative pressure of −0.075 MPa, a solid to liquid ratio of 1:20, 30 min of extraction and three extraction cycles. Under these optimized conditions, the extraction yields of VDL, CTR, VCR and VLB are 0.5783, 0.2843, 0.018 and 0.126 mg/g DW, respectively. These extraction yields are equivalent to those from the well-known ultrasonic extraction method and higher than the yields from maceration extraction and heating reflux extraction. Our results suggest that NPCE-RP-HPLC represents an excellent alternative for the extraction and quantification of vinca alkaloids for pilot- and industrial-scale applications.
Keywords: Catharanthus roseus; vinca alkaloids; negative-pressure cavitation extraction Catharanthus roseus; vinca alkaloids; negative-pressure cavitation extraction
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mu, F.; Yang, L.; Wang, W.; Luo, M.; Fu, Y.; Guo, X.; Zu, Y. Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from Catharanthus roseus Leaves. Molecules 2012, 17, 8742-8752.

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