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Molecules 2012, 17(8), 8742-8752; doi:10.3390/molecules17088742
Article

Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from Catharanthus roseus Leaves

1,2,3,†, 1,2,3,†, 1,2,3, 1,2,3,4,* , 1,2,3, 1,2,3 and 1,2,3,*
1 Engineering Research Center of Forestry Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, China 2 Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China 3 State Engineering Laboratory of Bio-Resource Eco-Utilization, Harbin 150040, China 4 Zhejiang Hisun Pharmaceutical Co. Ltd., Taizhou 318000, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 15 May 2012 / Revised: 11 July 2012 / Accepted: 13 July 2012 / Published: 25 July 2012
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Abstract

In the present study, an improved method termed negative-pressure cavitation extraction (NPCE) followed by reverse phase high-performance liquid chromatography (RP-HPLC) was developed for the extraction and quantification of vindoline (VDL), catharanthine (CTR), vincristine (VCR) and vinblastine (VLB) from Catharanthus roseus leaves. The optimized method employed 60-mesh particles, 80% ethanol, a negative pressure of −0.075 MPa, a solid to liquid ratio of 1:20, 30 min of extraction and three extraction cycles. Under these optimized conditions, the extraction yields of VDL, CTR, VCR and VLB are 0.5783, 0.2843, 0.018 and 0.126 mg/g DW, respectively. These extraction yields are equivalent to those from the well-known ultrasonic extraction method and higher than the yields from maceration extraction and heating reflux extraction. Our results suggest that NPCE-RP-HPLC represents an excellent alternative for the extraction and quantification of vinca alkaloids for pilot- and industrial-scale applications.
Keywords: Catharanthus roseus; vinca alkaloids; negative-pressure cavitation extraction Catharanthus roseus; vinca alkaloids; negative-pressure cavitation extraction
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Mu, F.; Yang, L.; Wang, W.; Luo, M.; Fu, Y.; Guo, X.; Zu, Y. Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from Catharanthus roseus Leaves. Molecules 2012, 17, 8742-8752.

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