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Molecules 2012, 17(7), 8276-8284; doi:10.3390/molecules17078276
Article

Preparative Isolation and Purification of Four Compounds from Cistanches deserticola Y.C. Ma by High-Speed Counter-Current Chromatography

1,2, 1, 2, 2,3, 2 and 1,*
1 Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Tianjin 300193, China 2 Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Tianjin 300193, China 3 Inner Mongolia Mandela Sand Industry Development Co., Ltd., Left Banner of Alashan, Inner Mongolia 750300, China
* Author to whom correspondence should be addressed.
Received: 23 May 2012 / Revised: 28 June 2012 / Accepted: 3 July 2012 / Published: 10 July 2012
(This article belongs to the Section Metabolites)
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Abstract

Following a constituent enrichment step on a silica gel column, four phenyl-ethanoid glycosides were successfully isolated from Cistanches deserticola and purified by preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of ethyl acetate-n-butanol-ethanol-water (40:6:6:50, v/v/v/v). A total of 30.9 mg acteoside, 13.0 mg isoacteoside, 12.5 mg syringalide A 3'-α-L-rhamnopyranoside and 7.2 mg 2'-acetylacteoside with purity of higher than 95%, as determined by HPLC-ELSD, were obtained in one-step separation from 297 mg of Cistanche deserticola extract, respectively. Their structures were identified by HR-MS, 1H-NMR and 13C-NMR.
Keywords: high-speed counter-current chromatography; Cistanches deserticola Y.C. Ma; phenylethanoid glycosides; acteoside; isoacteoside; syringalide A 3'-α-L-rhamnopyranoside; 2'-acetylacteoside high-speed counter-current chromatography; Cistanches deserticola Y.C. Ma; phenylethanoid glycosides; acteoside; isoacteoside; syringalide A 3'-α-L-rhamnopyranoside; 2'-acetylacteoside
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Han, L.; Ji, L.; Boakye-Yiadom, M.; Li, W.; Song, X.; Gao, X. Preparative Isolation and Purification of Four Compounds from Cistanches deserticola Y.C. Ma by High-Speed Counter-Current Chromatography. Molecules 2012, 17, 8276-8284.

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