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Molecules 2012, 17(7), 8276-8284; https://doi.org/10.3390/molecules17078276

Preparative Isolation and Purification of Four Compounds from Cistanches deserticola Y.C. Ma by High-Speed Counter-Current Chromatography

1
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Tianjin 300193, China
2
Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Tianjin 300193, China
3
Inner Mongolia Mandela Sand Industry Development Co., Ltd., Left Banner of Alashan, Inner Mongolia 750300, China
*
Author to whom correspondence should be addressed.
Received: 23 May 2012 / Revised: 28 June 2012 / Accepted: 3 July 2012 / Published: 10 July 2012
(This article belongs to the Section Natural Products Chemistry)
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Abstract

Following a constituent enrichment step on a silica gel column, four phenyl-ethanoid glycosides were successfully isolated from Cistanches deserticola and purified by preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of ethyl acetate-n-butanol-ethanol-water (40:6:6:50, v/v/v/v). A total of 30.9 mg acteoside, 13.0 mg isoacteoside, 12.5 mg syringalide A 3'-α-L-rhamnopyranoside and 7.2 mg 2'-acetylacteoside with purity of higher than 95%, as determined by HPLC-ELSD, were obtained in one-step separation from 297 mg of Cistanche deserticola extract, respectively. Their structures were identified by HR-MS, 1H-NMR and 13C-NMR.
Keywords: high-speed counter-current chromatography; Cistanches deserticola Y.C. Ma; phenylethanoid glycosides; acteoside; isoacteoside; syringalide A 3'-α-L-rhamnopyranoside; 2'-acetylacteoside high-speed counter-current chromatography; Cistanches deserticola Y.C. Ma; phenylethanoid glycosides; acteoside; isoacteoside; syringalide A 3'-α-L-rhamnopyranoside; 2'-acetylacteoside
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Han, L.; Ji, L.; Boakye-Yiadom, M.; Li, W.; Song, X.; Gao, X. Preparative Isolation and Purification of Four Compounds from Cistanches deserticola Y.C. Ma by High-Speed Counter-Current Chromatography. Molecules 2012, 17, 8276-8284.

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