Preparative Isolation and Purification of Four Compounds from Cistanches deserticola Y.C. Ma by High-Speed Counter-Current Chromatography
Abstract
:1. Introduction
2. Results and Discussion
2.1. HPLC Analysis of the Crude Extract
2.2. Selection of Two-Phase Solvent System
Solvent systems | K1 | K2 | K3 | K4 |
---|---|---|---|---|
ethyl acetate– n-butanol–ethanol–water (35:7:6:50, v/v/v/v) | 1.78 | 2.96 | 3.00 | 5.37 |
ethyl acetate– n-butanol–ethanol–water (40:6:7:45, v/v/v/v) | 1.68 | 2.42 | 3.01 | 4.3 |
ethyl acetate– n-butanol–ethanol–water (40:6:5:55, v/v/v/v) | 1.48 | 2.94 | 2.33 | 3.49 |
ethyl acetate– n-butanol–ethanol–water (45:5:6:50, v/v/v/v) | 1.12 | 1.66 | 3.10 | 3.10 |
ethyl acetate– n-butanol–ethanol–water (40:6:6:50, v/v/v/v) | 1.20 | 1.29 | 2.56 | 3.66 |
3. Experimental
3.1. Apparatus
3.2. Reagents and Materials
3.3. Preparation of Crude Sample
3.4. Selection of the Two-Phase-Solvent Systems
3.5. Preparation of Two-Phase-Solvent System and Sample Solution
3.6. HSCCC Separation Procedure
3.7. HPLC Analysis and Identification of HSCCC Peak Fractions
3.8. The Structural Identification
4. Conclusions
Acknowledgements
References
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Han, L.; Ji, L.; Boakye-Yiadom, M.; Li, W.; Song, X.; Gao, X. Preparative Isolation and Purification of Four Compounds from Cistanches deserticola Y.C. Ma by High-Speed Counter-Current Chromatography. Molecules 2012, 17, 8276-8284. https://doi.org/10.3390/molecules17078276
Han L, Ji L, Boakye-Yiadom M, Li W, Song X, Gao X. Preparative Isolation and Purification of Four Compounds from Cistanches deserticola Y.C. Ma by High-Speed Counter-Current Chromatography. Molecules. 2012; 17(7):8276-8284. https://doi.org/10.3390/molecules17078276
Chicago/Turabian StyleHan, Lifeng, Lina Ji, Mavis Boakye-Yiadom, Wei Li, Xinbo Song, and Xiumei Gao. 2012. "Preparative Isolation and Purification of Four Compounds from Cistanches deserticola Y.C. Ma by High-Speed Counter-Current Chromatography" Molecules 17, no. 7: 8276-8284. https://doi.org/10.3390/molecules17078276