Molecules 2012, 17(5), 5836-5853; doi:10.3390/molecules17055836
Article

Chemical Investigation of Saponins in Different Parts of Panax notoginseng by Pressurized Liquid Extraction and Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry

1,2, 1,2, 2, 1,2, 1,2,* email and 1,2,* email
Received: 12 April 2012; in revised form: 3 May 2012 / Accepted: 11 May 2012 / Published: 16 May 2012
(This article belongs to the Section Metabolites)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A pressurized liquid extraction (PLE) and high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed for the qualitative determination of saponins in different parts of P. notoginseng, including rhizome, root, fibre root, seed, stem, leaf and flower. The samples were extracted using PLE. The analysis was achieved on a Zorbax SB-C18 column with gradient elution of acetonitrile and 8 mM aqueous ammonium acetate as mobile phase. The mass spectrometer was operated in the negative ion mode using the electrospray ionization, and a collision induced dissociation (CID) experiment was also carried out to aid the identification of compounds. Forty one saponins were identified in different parts of P. notoginseng according to the fragmentation patterns and literature reports, among them, 21 saponins were confirmed by comparing the retention time and ESI-MS data with those of standard compounds. The results showed that the chemical characteristics were obviously diverse in different parts of P. notoginseng, which is helpful for pharmacological evaluation and quality control of P. notoginseng.
Keywords: different parts of Panax notoginseng; saponin; pressurized liquid extraction; liquid chromatography-mass spectrometry
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MDPI and ACS Style

Wan, J.-B.; Zhang, Q.-W.; Hong, S.-J.; Li, P.; Li, S.-P.; Wang, Y.-T. Chemical Investigation of Saponins in Different Parts of Panax notoginseng by Pressurized Liquid Extraction and Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry. Molecules 2012, 17, 5836-5853.

AMA Style

Wan J-B, Zhang Q-W, Hong S-J, Li P, Li S-P, Wang Y-T. Chemical Investigation of Saponins in Different Parts of Panax notoginseng by Pressurized Liquid Extraction and Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry. Molecules. 2012; 17(5):5836-5853.

Chicago/Turabian Style

Wan, Jian-Bo; Zhang, Qing-Wen; Hong, Si-Jia; Li, Peng; Li, Shao-Ping; Wang, Yi-Tao. 2012. "Chemical Investigation of Saponins in Different Parts of Panax notoginseng by Pressurized Liquid Extraction and Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry." Molecules 17, no. 5: 5836-5853.

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