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Optimization of a Microwave-Coupled Enzymatic Digestion Process to Prepare Peanut Peptides
Shandong Normal University, Jingshi Road No. 167, Lixia District, Jinan 250014, China
Shandong Peanut Research Institute, Wannianquan Road No. 126, Licang District, Qingdao 266100, China
* Author to whom correspondence should be addressed.
Received: 14 March 2012; in revised form: 3 May 2012 / Accepted: 7 May 2012 / Published: 11 May 2012
Abstract: The best enzyme to prepare peanut peptides, papain, coupled with microwave irradiation was selected from five common proteases according to the results of the yield of peanut peptides [nitrogen solution index (NSI) in trichloroacetic acid (TCA), TCA-NSI] and the degree of hydrolysis (DH). The main factors that influenced the microwave-coupled enzymatic digestion method were optimized by response surface analysis. The optimal conditions obtained were as follows: microwave extraction time, 9.5 min; power, 600 W; substrate concentration, 4%; enzymatic reaction temperature, 50 °C; enzyme quantity, 6,500 U/g; pH, 7.1 (phosphate buffer, 0.05 mol/L). Under these conditions, TCA-NSI was 62.00% and DH was 25.89%, which is higher than that obtained with either protease hydrolysis or microwave hydrolysis alone.
Keywords: microwave; peanut peptides; peanut protein; response surface methodology
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MDPI and ACS Style
Zhang, H.; Yu, L.; Yang, Q.; Sun, J.; Bi, J.; Liu, S.; Zhang, C.; Tang, L. Optimization of a Microwave-Coupled Enzymatic Digestion Process to Prepare Peanut Peptides. Molecules 2012, 17, 5661-5674.
Zhang H, Yu L, Yang Q, Sun J, Bi J, Liu S, Zhang C, Tang L. Optimization of a Microwave-Coupled Enzymatic Digestion Process to Prepare Peanut Peptides. Molecules. 2012; 17(5):5661-5674.
Zhang, Huicui; Yu, Lina; Yang, Qingli; Sun, Jie; Bi, Jie; Liu, Shaofang; Zhang, Chushu; Tang, Lin. 2012. "Optimization of a Microwave-Coupled Enzymatic Digestion Process to Prepare Peanut Peptides." Molecules 17, no. 5: 5661-5674.