Molecules 2012, 17(4), 4583-4594; doi:10.3390/molecules17044583
Article

Isolation and Identification of Myo-Inositol Crystals from Dragon Fruit (Hylocereus polyrhizus)

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Received: 1 March 2012; in revised form: 5 April 2012 / Accepted: 6 April 2012 / Published: 17 April 2012
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Crystals isolated from Hylocereus polyrhizus were analyzed using four different approaches—X-ray Crystallography, High Performance Liquid Chromatography (HPLC), Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) and Nuclear Magnetic Resonance (NMR) and identified as myo-inositol. The X-ray crystallography analysis showed that the unit-cell parameters were: a = 6.6226 (3) Å, b = 12.0462 (5) Å, c = 18.8942 (8) Å, α = 90.00, β = 93.98, δ = 90.00. The purity of the crystals were checked using HPLC, whereupon a clean single peak was obtained at 4.8 min with a peak area of 41232 μV*s. The LC-MS/MS technique, which is highly sensitive and selective, was used to provide a comparison of the isolated crystals with a myo-inositol standard where the results gave an identical match for both precursor and product ions. NMR was employed to confirm the molecular structure and conformation of the crystals, and the results were in agreement with the earlier results in this study. The discovery of myo-inositol crystals in substantial amount in H. polyrhizus has thus far not been reported and this is an important finding which will increase the marketability and importance of H. polyrhizus as a crop with a wide array of health properties.
Keywords: dragon fruit; crystals; Hylocereus polyrhizus; myo-inositol
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MDPI and ACS Style

Rebecca, O.P.S.; Boyce, A.N.; Somasundram, C. Isolation and Identification of Myo-Inositol Crystals from Dragon Fruit (Hylocereus polyrhizus). Molecules 2012, 17, 4583-4594.

AMA Style

Rebecca OPS, Boyce AN, Somasundram C. Isolation and Identification of Myo-Inositol Crystals from Dragon Fruit (Hylocereus polyrhizus). Molecules. 2012; 17(4):4583-4594.

Chicago/Turabian Style

Rebecca, Ow Phui San; Boyce, Amru Nasrulhaq; Somasundram, Chandran. 2012. "Isolation and Identification of Myo-Inositol Crystals from Dragon Fruit (Hylocereus polyrhizus)." Molecules 17, no. 4: 4583-4594.

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