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Isolation and Identification of Myo-Inositol Crystals from Dragon Fruit (Hylocereus polyrhizus)
Institute of Biological Sciences, Faculty of Science and Centre for Research in Biotechnology for Agriculture (CEBAR), University of Malaya, 50603 Kuala Lumpur, Malaysia
* Author to whom correspondence should be addressed.
Received: 1 March 2012; in revised form: 5 April 2012 / Accepted: 6 April 2012 / Published: 17 April 2012
Abstract: Crystals isolated from Hylocereus polyrhizus were analyzed using four different approaches—X-ray Crystallography, High Performance Liquid Chromatography (HPLC), Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) and Nuclear Magnetic Resonance (NMR) and identified as myo-inositol. The X-ray crystallography analysis showed that the unit-cell parameters were: a = 6.6226 (3) Å, b = 12.0462 (5) Å, c = 18.8942 (8) Å, α = 90.00, β = 93.98, δ = 90.00. The purity of the crystals were checked using HPLC, whereupon a clean single peak was obtained at 4.8 min with a peak area of 41232 μV*s. The LC-MS/MS technique, which is highly sensitive and selective, was used to provide a comparison of the isolated crystals with a myo-inositol standard where the results gave an identical match for both precursor and product ions. NMR was employed to confirm the molecular structure and conformation of the crystals, and the results were in agreement with the earlier results in this study. The discovery of myo-inositol crystals in substantial amount in H. polyrhizus has thus far not been reported and this is an important finding which will increase the marketability and importance of H. polyrhizus as a crop with a wide array of health properties.
Keywords: dragon fruit; crystals; Hylocereus polyrhizus; myo-inositol
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Rebecca, O.P.S.; Boyce, A.N.; Somasundram, C. Isolation and Identification of Myo-Inositol Crystals from Dragon Fruit (Hylocereus polyrhizus). Molecules 2012, 17, 4583-4594.
Rebecca OPS, Boyce AN, Somasundram C. Isolation and Identification of Myo-Inositol Crystals from Dragon Fruit (Hylocereus polyrhizus). Molecules. 2012; 17(4):4583-4594.
Rebecca, Ow Phui San; Boyce, Amru Nasrulhaq; Somasundram, Chandran. 2012. "Isolation and Identification of Myo-Inositol Crystals from Dragon Fruit (Hylocereus polyrhizus)." Molecules 17, no. 4: 4583-4594.