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Molecules 2012, 17(3), 3574-3585; doi:10.3390/molecules17033574

Regulation of Inflammatory Cytokines in Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage by 7-O-Methyl-naringenin

1,†, 2,†, 3,†, 1, 1, 1, 1,4,* , 1,4,*  and 1,*
1 College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China 2 ChangChun Central Hospital, Changchun 130051, Jilin, China 3 College of Life Science and Technology, Kunming University of Science and Technology, Kunming 650224, China 4 Laboratory of Nutrition and Function Food, Jilin University, Changchun 130062, Jilin, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 4 January 2012 / Revised: 27 February 2012 / Accepted: 15 March 2012 / Published: 22 March 2012
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7-O-Methylnaringenin, extracted from Rhododendron speciferum, belongs to the flavanone class of polyphenols. In the present study, we investigated the anti-inflammatory effects of 7-O-methylnaringenin on cytokine production by lipopoly-saccharide (LPS)-stimulated RAW 264.7 macrophages in vitro. The results showed that pretreatment with 10, 20 or 40 μg/mL of 7-O-methylnaringenin could downregulate tumour necrosis factor (TNF-α), interleukin (IL-6) and interleukin (IL-1β) in a dose-dependent manner. Furthermore, we investigated the signal transduction mechanisms to determine how 7-O-methylnaringenin affects RAW 264.7 macrophages. The activation of mitogen-activated protein kinases (MAPK) and IκBα were measured by Western blotting. The data showed that 7-O-methylnaringenin could downregulate LPS-induced levels of phosphorylation of ERK1/2, JNK and IκBα. These observations indicated that 7-O-methylnaringenin modulated inflammatory cytokine responses by blocking NF-қB, ERK1/2 and JNK/MAPKs activation.
Keywords: 7-O-methylnaringenin; anti-inflammation; signal pathways; cytokines 7-O-methylnaringenin; anti-inflammation; signal pathways; cytokines
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Soromou, L.W.; Zhang, Z.; Li, R.; Chen, N.; Guo, W.; Huo, M.; Guan, S.; Lu, J.; Deng, X. Regulation of Inflammatory Cytokines in Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage by 7-O-Methyl-naringenin. Molecules 2012, 17, 3574-3585.

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