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Molecules 2012, 17(3), 3574-3585; doi:10.3390/molecules17033574
Article

Regulation of Inflammatory Cytokines in Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage by 7-O-Methyl-naringenin

1,†, 2,†, 3,†, 1, 1, 1, 1,4,* , 1,4,*  and 1,*
1 College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China 2 ChangChun Central Hospital, Changchun 130051, Jilin, China 3 College of Life Science and Technology, Kunming University of Science and Technology, Kunming 650224, China 4 Laboratory of Nutrition and Function Food, Jilin University, Changchun 130062, Jilin, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 4 January 2012 / Revised: 27 February 2012 / Accepted: 15 March 2012 / Published: 22 March 2012
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Abstract

7-O-Methylnaringenin, extracted from Rhododendron speciferum, belongs to the flavanone class of polyphenols. In the present study, we investigated the anti-inflammatory effects of 7-O-methylnaringenin on cytokine production by lipopoly-saccharide (LPS)-stimulated RAW 264.7 macrophages in vitro. The results showed that pretreatment with 10, 20 or 40 μg/mL of 7-O-methylnaringenin could downregulate tumour necrosis factor (TNF-α), interleukin (IL-6) and interleukin (IL-1β) in a dose-dependent manner. Furthermore, we investigated the signal transduction mechanisms to determine how 7-O-methylnaringenin affects RAW 264.7 macrophages. The activation of mitogen-activated protein kinases (MAPK) and IκBα were measured by Western blotting. The data showed that 7-O-methylnaringenin could downregulate LPS-induced levels of phosphorylation of ERK1/2, JNK and IκBα. These observations indicated that 7-O-methylnaringenin modulated inflammatory cytokine responses by blocking NF-қB, ERK1/2 and JNK/MAPKs activation.
Keywords: 7-O-methylnaringenin; anti-inflammation; signal pathways; cytokines 7-O-methylnaringenin; anti-inflammation; signal pathways; cytokines
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Soromou, L.W.; Zhang, Z.; Li, R.; Chen, N.; Guo, W.; Huo, M.; Guan, S.; Lu, J.; Deng, X. Regulation of Inflammatory Cytokines in Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage by 7-O-Methyl-naringenin. Molecules 2012, 17, 3574-3585.

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