Molecules 2012, 17(3), 3148-3154; doi:10.3390/molecules17033148
Article

Hydrolysis of Ibuprofen Nitrile and Ibuprofen Amide and Deracemisation of Ibuprofen Using Nocardia corallina B-276

1, 1,* email, 1, 1 and 2
Received: 31 January 2012; in revised form: 6 March 2012 / Accepted: 7 March 2012 / Published: 12 March 2012
(This article belongs to the Section Theoretical Chemistry)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A novel application of whole cells of Nocardia corallina B-276 for the deracemisation of ibuprofen is reported. This microorganism successfully hydrolysed ibuprofen nitrile to ibuprofen amide, and ibuprofen amide to ibuprofen, using a suspension of cells in a potassium phosphate buffer solution (0.1 M, pH = 7.0). These results can be explained by the presence of NHase and amidase enzymes, but the reactions are not enantioselective and low ee values were obtained. However, (R)-ibuprofen was isolated with >99% ee by a deracemisation process catalysed by N. corallina B-276. This is the first report of this kind of catalysis with this microorganism.
Keywords: Nocardia corallina; deracemisation; ibuprofen; nitrile hydratase; amidase
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MDPI and ACS Style

Lievano, R.; Pérez, H.I.; Manjarrez, N.; Solís, A.; Solís-Oba, M. Hydrolysis of Ibuprofen Nitrile and Ibuprofen Amide and Deracemisation of Ibuprofen Using Nocardia corallina B-276. Molecules 2012, 17, 3148-3154.

AMA Style

Lievano R, Pérez HI, Manjarrez N, Solís A, Solís-Oba M. Hydrolysis of Ibuprofen Nitrile and Ibuprofen Amide and Deracemisation of Ibuprofen Using Nocardia corallina B-276. Molecules. 2012; 17(3):3148-3154.

Chicago/Turabian Style

Lievano, Ricardo; Pérez, Herminia Inés; Manjarrez, Norberto; Solís, Aida; Solís-Oba, Myrna. 2012. "Hydrolysis of Ibuprofen Nitrile and Ibuprofen Amide and Deracemisation of Ibuprofen Using Nocardia corallina B-276." Molecules 17, no. 3: 3148-3154.

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