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Molecules 2012, 17(3), 3155-3164; doi:10.3390/molecules17033155

Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells

1
Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China
2
Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China
3
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
4
School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
*
Author to whom correspondence should be addressed.
Received: 6 February 2012 / Revised: 28 February 2012 / Accepted: 2 March 2012 / Published: 12 March 2012
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Abstract

Astragalus polysaccharides (APS), one of main bioactive components in Astragalus membranaceus Bunge, has been reported to possess anti-inflammatory activities, but the molecular mechanisms behind this activity are largely unknown. This study aimed to investigate expression of inflammatory cytokines and the MAPK/NF-κB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS). The results showed that the concentrations of TNF-a and IL-1β released from LPS stimulated THP-1 cells increased significantly compared to control (p < 0.01). After treatment with APS, the TNF-a and IL-1β levels were significantly lower than those in the LPS group (p < 0.05). The mRNA expression of TNF-a and IL-1β were also inhibited. Mechanistic studies indicated that APS strongly suppressed NF-κB activation and down-regulated the phosphorylation of ERK and JNK, which are important signaling pathways involved in the production of TNF-a and IL-1β, demonstrating that APS could suppress the production of TNF-a and IL-1β by LPS stimulated macrophages by inhibiting NF-κB activation and ERK and JNK phosphorylation.
Keywords: Astragalus polysaccharides; anti-inflammatory; macrophage; MAPK; NF-κB Astragalus polysaccharides; anti-inflammatory; macrophage; MAPK; NF-κB
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

He, X.; Shu, J.; Xu, L.; Lu, C.; Lu, A. Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells. Molecules 2012, 17, 3155-3164.

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