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Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells
Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China
Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
* Author to whom correspondence should be addressed.
Received: 6 February 2012; in revised form: 28 February 2012 / Accepted: 2 March 2012 / Published: 12 March 2012
Abstract: Astragalus polysaccharides (APS), one of main bioactive components in Astragalus membranaceus Bunge, has been reported to possess anti-inflammatory activities, but the molecular mechanisms behind this activity are largely unknown. This study aimed to investigate expression of inflammatory cytokines and the MAPK/NF-κB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS). The results showed that the concentrations of TNF-a and IL-1β released from LPS stimulated THP-1 cells increased significantly compared to control (p < 0.01). After treatment with APS, the TNF-a and IL-1β levels were significantly lower than those in the LPS group (p < 0.05). The mRNA expression of TNF-a and IL-1β were also inhibited. Mechanistic studies indicated that APS strongly suppressed NF-κB activation and down-regulated the phosphorylation of ERK and JNK, which are important signaling pathways involved in the production of TNF-a and IL-1β, demonstrating that APS could suppress the production of TNF-a and IL-1β by LPS stimulated macrophages by inhibiting NF-κB activation and ERK and JNK phosphorylation.
Keywords: Astragalus polysaccharides; anti-inflammatory; macrophage; MAPK; NF-κB
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MDPI and ACS Style
He, X.; Shu, J.; Xu, L.; Lu, C.; Lu, A. Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells. Molecules 2012, 17, 3155-3164.
He X, Shu J, Xu L, Lu C, Lu A. Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells. Molecules. 2012; 17(3):3155-3164.
He, Xiaojuan; Shu, Jun; Xu, Li; Lu, Cheng; Lu, Aiping. 2012. "Inhibitory Effect of Astragalus Polysaccharides on Lipopolysaccharide-Induced TNF-a and IL-1β Production in THP-1 Cells." Molecules 17, no. 3: 3155-3164.