Molecules 2012, 17(3), 3093-3113; doi:10.3390/molecules17033093
Article

Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species

* email, email and email
Received: 15 February 2012; in revised form: 5 March 2012 / Accepted: 6 March 2012 / Published: 12 March 2012
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The antioxidant efficiency of dry extracts from inflorescences and/or leaves of seven Sorbus species was studied using four in vitro tests of SET (single electron transfer) and HAT-type (hydrogen atom transfer) mechanisms. The 70% methanol extracts and its diethyl ether, ethyl acetate, n-butanol and water fractions were tested in parallel with the phenolic standards, e.g., caffeic acid, quercetin, BHA, BHT, and TroloxÒ. The SET-type activity of the extracts depended primarily on the extraction solvent. The most valuable extracts were n-butanol and ethyl acetate ones, which activity was high in the DPPH (EC50 = 3.2–5.2 μg/mL), TEAC (2.8–4.0 mmol Trolox®/g), and FRAP (9.8–13.7 mmol Fe2+/g) tests, and strongly correlated with the total phenolic levels (39.6–58.2% of gallic acid equivalents). The HPLC-PDA analysis of the extracts led to the identification of chlorogenic acid, isoquercitrin, hyperoside, rutin, quercetin 3-O-sophoroside, and sexangularetin 3-O-b-D-glucopyranoside as the main components. Apart from flavonoids and hydroxycinnamic acids, proanthocyanidins have also a significant impact on the SET-type activity. The HAT-reactivity of the extracts in the linoleic acid peroxidation test (IC50 = 36.9–228.3 μg/mL) depended more strongly on the plant tissue than on the extraction solvent, and its correlation with the phenolic content was weak. Both SET and HAT-type activity of the most potent Sorbus extracts was comparable with the activity of the standards, indicating their great potential as effective sources for health products.
Keywords: Sorbus; antioxidant activity; scavenging capacity; reducing power; linoleic acid peroxidation; DPPH; FRAP; TEAC; phenolic content; HPLC
PDF Full-text Download PDF Full-Text [490 KB, Updated Version, uploaded 18 June 2014 19:45 CEST]
The original version is still available [489 KB, uploaded 18 June 2014 19:45 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Olszewska, M.A.; Presler, A.; Michel, P. Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species. Molecules 2012, 17, 3093-3113.

AMA Style

Olszewska MA, Presler A, Michel P. Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species. Molecules. 2012; 17(3):3093-3113.

Chicago/Turabian Style

Olszewska, Monika A.; Presler, Anna; Michel, Piotr. 2012. "Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species." Molecules 17, no. 3: 3093-3113.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert