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Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Lodz, Muszyńskiego 1, 90 151 Lodz, Poland
* Author to whom correspondence should be addressed.
Received: 15 February 2012; in revised form: 5 March 2012 / Accepted: 6 March 2012 / Published: 12 March 2012
Abstract: The antioxidant efficiency of dry extracts from inflorescences and/or leaves of seven Sorbus species was studied using four in vitro tests of SET (single electron transfer) and HAT-type (hydrogen atom transfer) mechanisms. The 70% methanol extracts and its diethyl ether, ethyl acetate, n-butanol and water fractions were tested in parallel with the phenolic standards, e.g., caffeic acid, quercetin, BHA, BHT, and TroloxÒ. The SET-type activity of the extracts depended primarily on the extraction solvent. The most valuable extracts were n-butanol and ethyl acetate ones, which activity was high in the DPPH (EC50 = 3.2–5.2 μg/mL), TEAC (2.8–4.0 mmol Trolox®/g), and FRAP (9.8–13.7 mmol Fe2+/g) tests, and strongly correlated with the total phenolic levels (39.6–58.2% of gallic acid equivalents). The HPLC-PDA analysis of the extracts led to the identification of chlorogenic acid, isoquercitrin, hyperoside, rutin, quercetin 3-O-sophoroside, and sexangularetin 3-O-b-D-glucopyranoside as the main components. Apart from flavonoids and hydroxycinnamic acids, proanthocyanidins have also a significant impact on the SET-type activity. The HAT-reactivity of the extracts in the linoleic acid peroxidation test (IC50 = 36.9–228.3 μg/mL) depended more strongly on the plant tissue than on the extraction solvent, and its correlation with the phenolic content was weak. Both SET and HAT-type activity of the most potent Sorbus extracts was comparable with the activity of the standards, indicating their great potential as effective sources for health products.
Keywords: Sorbus; antioxidant activity; scavenging capacity; reducing power; linoleic acid peroxidation; DPPH; FRAP; TEAC; phenolic content; HPLC
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Olszewska, M.A.; Presler, A.; Michel, P. Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species. Molecules 2012, 17, 3093-3113.
Olszewska MA, Presler A, Michel P. Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species. Molecules. 2012; 17(3):3093-3113.
Olszewska, Monika A.; Presler, Anna; Michel, Piotr. 2012. "Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species." Molecules 17, no. 3: 3093-3113.