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Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species
AbstractThe antioxidant efficiency of dry extracts from inflorescences and/or leaves of seven Sorbus species was studied using four in vitro tests of SET (single electron transfer) and HAT-type (hydrogen atom transfer) mechanisms. The 70% methanol extracts and its diethyl ether, ethyl acetate, n-butanol and water fractions were tested in parallel with the phenolic standards, e.g., caffeic acid, quercetin, BHA, BHT, and TroloxÒ. The SET-type activity of the extracts depended primarily on the extraction solvent. The most valuable extracts were n-butanol and ethyl acetate ones, which activity was high in the DPPH (EC50 = 3.2–5.2 μg/mL), TEAC (2.8–4.0 mmol Trolox®/g), and FRAP (9.8–13.7 mmol Fe2+/g) tests, and strongly correlated with the total phenolic levels (39.6–58.2% of gallic acid equivalents). The HPLC-PDA analysis of the extracts led to the identification of chlorogenic acid, isoquercitrin, hyperoside, rutin, quercetin 3-O-sophoroside, and sexangularetin 3-O-b-D-glucopyranoside as the main components. Apart from flavonoids and hydroxycinnamic acids, proanthocyanidins have also a significant impact on the SET-type activity. The HAT-reactivity of the extracts in the linoleic acid peroxidation test (IC50 = 36.9–228.3 μg/mL) depended more strongly on the plant tissue than on the extraction solvent, and its correlation with the phenolic content was weak. Both SET and HAT-type activity of the most potent Sorbus extracts was comparable with the activity of the standards, indicating their great potential as effective sources for health products.
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Olszewska, M.A.; Presler, A.; Michel, P. Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species. Molecules 2012, 17, 3093-3113.View more citation formats
Olszewska MA, Presler A, Michel P. Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species. Molecules. 2012; 17(3):3093-3113.Chicago/Turabian Style
Olszewska, Monika A.; Presler, Anna; Michel, Piotr. 2012. "Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species." Molecules 17, no. 3: 3093-3113.