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Molecules 2012, 17(3), 3093-3113; doi:10.3390/molecules17033093
Article

Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species

* ,
 and
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Lodz, Muszyńskiego 1, 90 151 Lodz, Poland
* Author to whom correspondence should be addressed.
Received: 15 February 2012 / Revised: 5 March 2012 / Accepted: 6 March 2012 / Published: 12 March 2012
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Abstract

The antioxidant efficiency of dry extracts from inflorescences and/or leaves of seven Sorbus species was studied using four in vitro tests of SET (single electron transfer) and HAT-type (hydrogen atom transfer) mechanisms. The 70% methanol extracts and its diethyl ether, ethyl acetate, n-butanol and water fractions were tested in parallel with the phenolic standards, e.g., caffeic acid, quercetin, BHA, BHT, and TroloxÒ. The SET-type activity of the extracts depended primarily on the extraction solvent. The most valuable extracts were n-butanol and ethyl acetate ones, which activity was high in the DPPH (EC50 = 3.2–5.2 μg/mL), TEAC (2.8–4.0 mmol Trolox®/g), and FRAP (9.8–13.7 mmol Fe2+/g) tests, and strongly correlated with the total phenolic levels (39.6–58.2% of gallic acid equivalents). The HPLC-PDA analysis of the extracts led to the identification of chlorogenic acid, isoquercitrin, hyperoside, rutin, quercetin 3-O-sophoroside, and sexangularetin 3-O-b-D-glucopyranoside as the main components. Apart from flavonoids and hydroxycinnamic acids, proanthocyanidins have also a significant impact on the SET-type activity. The HAT-reactivity of the extracts in the linoleic acid peroxidation test (IC50 = 36.9–228.3 μg/mL) depended more strongly on the plant tissue than on the extraction solvent, and its correlation with the phenolic content was weak. Both SET and HAT-type activity of the most potent Sorbus extracts was comparable with the activity of the standards, indicating their great potential as effective sources for health products.
Keywords: Sorbus; antioxidant activity; scavenging capacity; reducing power; linoleic acid peroxidation; DPPH; FRAP; TEAC; phenolic content; HPLC Sorbus; antioxidant activity; scavenging capacity; reducing power; linoleic acid peroxidation; DPPH; FRAP; TEAC; phenolic content; HPLC
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Olszewska, M.A.; Presler, A.; Michel, P. Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species. Molecules 2012, 17, 3093-3113.

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