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Effects of Pithecellobium Jiringa Ethanol Extract against Ethanol-Induced Gastric Mucosal Injuries in Sprague-Dawley Rats
Department of Pharmacology, Faculty of Medicine, Universiti Teknology MARA, Shah Alam 40450, Malaysia
Department of Biological Science, Faculty of Biosciences and Bioengineering, University of Technology Malaysia, UTM Skudai 81310, Johor, Malaysia
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia
Division of Basic Medical Sciences, Faculty of Medicine, Cyberjaya University College of Medical Sciences (CUCMS), Cyberjaya 63000, Selangor, Malaysia
Department of Pharmacy, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia
Department of Restorative Dentistry, Faculty of Dentistry, Universiti Technology MARA, Shah Alam 40450, Malaysia
* Author to whom correspondence should be addressed.
Received: 31 December 2011; in revised form: 23 February 2012 / Accepted: 23 February 2012 / Published: 6 March 2012
Abstract: Current anti-gastric ulcer agents have side effects, despite the progression and expansion of advances in treatment. This study aimed to investigate the gastroprotective mechanisms of Pithecellobium jiringa ethanol extract against ethanol-induced gastric mucosal ulcers in rats. For this purpose, Sprague Dawley rats were randomly divided into five groups: Group 1 (normal control) rats were orally administered with vehicle (carboxymethyl cellulose), Group 2 (ulcer control) rats were also orally administered with vehicle. Group 3 (positive control) rats were orally administered with 20 mg/kg omeprazole, Groups 4 and 5 (experimental groups) received ethanol extract of Pithecellobium jiringa ethanol extract at a concentration of 250 and 500 mg/kg, respectively. Sixty minutes later, vehicle was given orally to the normal control group, and absolute ethanol was given orally to the ulcer control, positive control and experimental groups to generate gastric mucosal injury. The rats were sacrificed an hour later. The effect of oral administration of plant extract on ethanol-induced gastric mucosal injury was studied grossly and histology. The level of lipid peroxidation (malondialdehyde—MDA), superoxide dismutase (SOD) and gastric wall mucus were measured from gastric mucosal homogenate. The ulcer control group exhibited severe gastric mucosal injury, and this finding was also confirmed by histology of gastric mucosa which showed severe damage to the gastric mucosa with edema and leucocyte infiltration of the submucosal layer. Pre-treatment with plant extract significantly reduced the formation of ethanol-induced gastric lesions, and gastric wall mucus was significantly preserved. The study also indicated a significant increase in SOD activity in gastric mucosal homogenate, whereas a significant decrease in MDA was observed. Acute toxicity tests did not show any signs of toxicity and mortality up to 5 g/kg. The ulcer protective effect of this plant may possibly be due to its preservation of gastric wall mucus along with increased SOD activity and reduction of oxidative stress (MDA). The extract is non-toxic, even at relatively high concentrations.
Keywords: Pithecellobium jiringa; gastric ulcers; ethanol; omeprazole; histology
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Aziz Ibrahim, I.A.; Qader, S.W.; Abdulla, M.A.; Nimir, A.R.; Abdelwahab, S.I.; AL-Bayaty, F.H. Effects of Pithecellobium Jiringa Ethanol Extract against Ethanol-Induced Gastric Mucosal Injuries in Sprague-Dawley Rats. Molecules 2012, 17, 2796-2811.
Aziz Ibrahim IA, Qader SW, Abdulla MA, Nimir AR, Abdelwahab SI, AL-Bayaty FH. Effects of Pithecellobium Jiringa Ethanol Extract against Ethanol-Induced Gastric Mucosal Injuries in Sprague-Dawley Rats. Molecules. 2012; 17(3):2796-2811.
Aziz Ibrahim, Ibrahim Abdel; Qader, Suhailah Wasmn; Abdulla, Mahmood Ameen; Nimir, Amal R.; Abdelwahab, Siddig Ibrahim; AL-Bayaty, Fouad Hussain. 2012. "Effects of Pithecellobium Jiringa Ethanol Extract against Ethanol-Induced Gastric Mucosal Injuries in Sprague-Dawley Rats." Molecules 17, no. 3: 2796-2811.