Molecules 2012, 17(3), 2773-2783; doi:10.3390/molecules17032773
Article

Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola

Jan Tříska 1,* email, Naděžda Vrchotová 1 email, Julie Olejníčková 2 email, Rudolf Jílek 1 email and Radek Sotolář 3 email
1 Laboratory of Metabolomics and Isotopic Analyses, Global Change Research Center, Academy of Sciences of the Czech Republic, Branišovská 31, České Budějovice 370 05, Czech Republic 2 Laboratory of Plant Ecological Physiology, Global Change Research Center, Academy of Sciences of the Czech Republic, Zámek 136, Nové Hrady 373 33, Czech Republic 3 Department of Viticulture, Faculty of Horticulture, Mendel University in Brno, Valtická 337, Lednice 691 44, Czech Republic
* Author to whom correspondence should be addressed.
Received: 24 December 2011; in revised form: 27 February 2012 / Accepted: 28 February 2012 / Published: 6 March 2012
(This article belongs to the Section Natural Products)
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Abstract: A method for identification of highly fluorescent compounds in vine leaves infected by Plasmopara viticola was developed using reversed phase liquid chromatography with simultaneous diode array and fluorometric detection. Fluorescent compounds were extracted from leaves with a methanol-water mixture (70:30). Separation by HPLC was performed using a C18 column and gradient elution with water-acetonitrile mixtures (20–80% of acetonitrile). The main unknown fluorescent compound was identified by line spectral comparison with a standard obtained by UV photoisomerization of trans-resveratrol glucoside, and its structure was confirmed by liquid chromatography-mass spectrometry. Identification and structural elucidation of the fluorescent compound in the leaves of Vitis vinifera allows early detection of Plasmopara viticola invasion.
Keywords: trans-resveratrol; transformation; 2,4,6-trihydroxyphenanthrene-2-O-glucoside; Plasmopara viticola

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MDPI and ACS Style

Tříska, J.; Vrchotová, N.; Olejníčková, J.; Jílek, R.; Sotolář, R. Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola. Molecules 2012, 17, 2773-2783.

AMA Style

Tříska J, Vrchotová N, Olejníčková J, Jílek R, Sotolář R. Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola. Molecules. 2012; 17(3):2773-2783.

Chicago/Turabian Style

Tříska, Jan; Vrchotová, Naděžda; Olejníčková, Julie; Jílek, Rudolf; Sotolář, Radek. 2012. "Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola." Molecules 17, no. 3: 2773-2783.

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