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Flavone Enhances Dengue Virus Type-2 (NGC Strain) Infectivity and Replication in Vero Cells
Tropical Infectious Diseases Research and Education Center (TIDREC), Department of Medical Microbiology, Faculty of Medicine, University Malaya, Kuala Lumpur 50603, Malaysia
Department of Pharmacology, Faculty of Medicine, University Malaya, Kuala Lumpur 50603, Malaysia
* Author to whom correspondence should be addressed.
Received: 13 December 2011; in revised form: 29 January 2012 / Accepted: 31 January 2012 / Published: 28 February 2012
Abstract: This study investigates the effects of 2-phenyl-1-benzopyran-4-one (flavone) on DENV-2 infectivity in Vero cells. Virus adsorption and attachment and intracellular virus replication were investigated using a foci forming unit assay (FFUA) and quantitative RT-PCR, respectively. Addition of flavone (100 μg/mL) significantly increased the number of DENV-2 foci by 35.66% ± 1.52 and 49.66% ± 2.51 when added during and after virus adsorption to the Vero cells, respectively. The average foci size after 4 days of infection increased by 33% ± 2.11 and 89% ± 2.13. The DENV-2 specific RNA copy number in the flavone-treated infected cells increased by 6.41- and 23.1-fold when compared to the mock-treated infected cells. Flavone (100 μg/mL) did not promote or inhibit Vero cell proliferation. The CC50 value of flavone against Vero cells was 446 µg/mL. These results suggest that flavone might enhance dengue virus replication by acting antagonistically towards flavonoids known to inhibit dengue virus replication.
Keywords: antiviral; dengue; flavones; flavonoid; molecule
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Zandi, K.; Lani, R.; Wong, P.-F.; Teoh, B.-T.; Sam, S.-S.; Johari, J.; Mustafa, M.R.; AbuBakar, S. Flavone Enhances Dengue Virus Type-2 (NGC Strain) Infectivity and Replication in Vero Cells. Molecules 2012, 17, 2437-2445.
Zandi K, Lani R, Wong P-F, Teoh B-T, Sam S-S, Johari J, Mustafa MR, AbuBakar S. Flavone Enhances Dengue Virus Type-2 (NGC Strain) Infectivity and Replication in Vero Cells. Molecules. 2012; 17(3):2437-2445.
Zandi, Keivan; Lani, Rafidah; Wong, Pooi-Fong; Teoh, Boon-Teong; Sam, Sing-Sin; Johari, Jefree; Mustafa, Mohd Rais; AbuBakar, Sazaly. 2012. "Flavone Enhances Dengue Virus Type-2 (NGC Strain) Infectivity and Replication in Vero Cells." Molecules 17, no. 3: 2437-2445.