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Purification and Characterization of a Novel ~18 kDa Antioxidant Protein from Ginkgo biloba Seeds
Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, Jiangsu, China
2 National Engineering Lab for Biomass Chemical Utilization, Nanjing 210042, Jiangsu, China
Key and Open Lab on Forest Chemical Engineering, SFA, Nanjing 210042, Jiangsu, China
Key Lab of Biomass Energy and Material, Nanjing 210042, Jiangsu, China
Institute of New Technology of Forestry, CAF, Beijing 100091, China
* Author to whom correspondence should be addressed.
Received: 1 November 2012; in revised form: 30 November 2012 / Accepted: 7 December 2012 / Published: 11 December 2012
Abstract: Ginkgo biloba seeds are widely used as a food and traditional medicine in China. In the present study, a novel antioxidant protein named GBSP was purified from Ginkgo biloba seeds. The protein (GBSP) was purified by homogenization of Ginkgo biloba seed powder in saline solution, 70% ammonium sulphate precipitation, filtration on a DEAE-Cellulose52 anion exchange column, gel filtration on a Sephadex G-50 column, and preparative chromatography on a C18 column using RP-HPLC. GBSP showed an apparent molecular weight of 18 kDa by SDS-PAGE and MALDI-TOF/MS analyses. The amino acid sequence obtained by MALDI-TOF/TOF MS analysis showed GBSP was a novel protein, as no matching protein in was found the database. The protein exhibited significant antioxidant activities against free radicals such as DPPH, ABTS and superoxide anion and showed higher activity than α-tocopherol in a linoleic acid emulsion assay system. Furthermore, GBSP exhibited notable reducing power and a strong chelating effect on Cu2+and Fe2+. Therefore, the present study demonstrates, for the first time, that this novel protein from Ginkgo biloba seeds is an excellent antioxidant.
Keywords: Ginkgo biloba seeds; 18 kDa protein molecule; antioxidant activity
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MDPI and ACS Style
Zhou, H.; Chen, X.; Wang, C.; Ye, J.; Chen, H. Purification and Characterization of a Novel ~18 kDa Antioxidant Protein from Ginkgo biloba Seeds. Molecules 2012, 17, 14778-14794.
Zhou H, Chen X, Wang C, Ye J, Chen H. Purification and Characterization of a Novel ~18 kDa Antioxidant Protein from Ginkgo biloba Seeds. Molecules. 2012; 17(12):14778-14794.
Zhou, Hao; Chen, Xijuan; Wang, Chengzhang; Ye, Jianzhong; Chen, Hongxia. 2012. "Purification and Characterization of a Novel ~18 kDa Antioxidant Protein from Ginkgo biloba Seeds." Molecules 17, no. 12: 14778-14794.