Study of the Volatile Constituents in Radix Flemingiae Macrophyllae and a Substitute by Gas Chromatography-Mass Spectrometry and Chemometric Methods
Abstract
:1. Introduction
2. Results and Discussion
2.1. Resolution of Overlapped Peaks with SFA
Series No. | Retention time (min) | Compound name | Molecularformula | Relative content (%) | |
---|---|---|---|---|---|
X1 a | X2 b | ||||
1 | 3.439 | - | Prenal | C5H8O | 0.07 |
2 | 3.653 | 3.658 | Hexanal | C6H12O | 0.35 |
3 | 6.368 | 6.372 | 2-Amylfuran | C9H14O | 0.03 |
4 | 7.065 | - | D-Limonene | C9H20 | 0.27 |
5 | 7.979 | 7.984 | Linalool | C10H18O | 0.98 |
6 | 8.682 | 8.691 | L-Camphor | C10H16O | 1.91 |
7 | 9.104 | - | l-2-Bornanol | C10H18O | 0.06 |
8 | 9.267 | 9.275 | p-Menth-1-en-4-ol | C10H18O | 0.66 |
9 | 9.419 | 9.428 | p-Menth-1-en-8-ol | C10H18O | 0.87 |
10 | 10.529 | 10.536 | Nonanoic acid | C9H18O2 | 0.46 |
11 | 10.671 | - | Perillyl aldehyde | C10H14O | 0.04 |
12 | 10.914 | 10.925 | Anethole | C10H12O | 1.62 |
13 | 11.065 | 11.081 | Bornyl acetate | C12H20O2 | 0.72 |
14 | 11.294 | 11.308 | Tricyclo[3.2.1.02,7]oct-3-ene, 2,3,4,5-tetramethyl- | C12H 18 | 0.57 |
15 | 11.504 | - | Furan, 2,5-dibutyl- | C12H20O | 0.03 |
16 | 12.465 | 12.478 | Decanoic acid | C10H20O2 | 0.06 |
17 | 12.720 | 12.731 | α-Cubebene | C15H24 | 0.56 |
18 | 12.859 | 12.875 | α-Longipinene | C15H24 | 1.47 |
19 | 13.325 | 13.331 | Ylangene | C15H24 | 1.69 |
20 | 13.476 | 13.493 | Longicyclene | C15H24 | 2.84 |
21 | 13.771 | - | Copaene | C15H24 | 0.64 |
22 | 14.305 | 14.312 | Longofolene | C15H24 | 3.21 |
23 | 14.437 | - | Di-epi-α-cedrene | C15H26 | 0.08 |
24 | 14530 | 14.542 | β-Caryophyllene | C15H24 | 0.82 |
25 | 14.575 | 14.583 | β-Cedrene | C15H24 | 5.92 |
26 | 14.744 | 14.762 | Germacrene D | C15H24 | 0.65 |
27 | 15.057 | 15.071 | (Z)-β-Farnesene | C15H24 | 0.61 |
28 | 15.108 | - | Himachala-2,4-diene | C15H24 | 0.07 |
29 | 15.385 | 15.435 | α-Himachalene | C15H24 | 4.13 |
30 | 15.572 | 15.590 | α-Caryophyllene | C15H24 | 0.44 |
31 | 15.593 | - | Acoradiene | C15H24 | 0.33 |
32 | 15.673 | 15.685 | Dihydrocurcumene | C15H24 | 0.03 |
33 | 15.858 | 15.869 | Eremophilene | C15H24 | 1.30 |
34 | 16.006 | 16.021 | (+)-Cycloisosativene | C15H24 | 0.05 |
35 | 16.108 | 16.117 | Humulen-(v1) | C15H24 | 0.92 |
36 | 16.210 | 16.215 | Longifolene-(V4) | C15H24 | 6.82 |
37 | 16.321 | 16.327 | α-Guaiene | C15H24 | 0.49 |
38 | 16.419 | - | Patchoulene | C15H24 | 0.88 |
39 | 16.721 | 16.730 | β-Himachalene | C15H24 | 5.26 |
40 | 17.077 | 17.086 | β-Guaiene | C15H24 | 0.72 |
41 | 17.162 | 17.184 | Dihydroactinidiolide | C11H16O2 | 2.14 |
42 | 17.275 | 17.292 | δ-Cadinene | C15H24 | 1.53 |
43 | 17.321 | 17.334 | β-Cadinece | C15H24 | 0.56 |
44 | 17.510 | - | Valencene | C15H24 | 0.67 |
45 | 18.710 | 18.722 | Caryophyllenyl alcohol | C15H26O | 0.31 |
46 | 19.076 | 19.085 | Caryophyllene oxide | C15H24O | 0.85 |
47 | 19.142 | 19.155 | Drimenol | C15H26O | 1.27 |
48 | 19.470 | - | 3-Isobutyl-4,5-dimethyl-3H-isobenzofuran-1-one | C14H18O2 | 0.06 |
49 | 19.732 | 19.740 | Cedrol | C15H26O | 0.83 |
50 | 19.918 | 19.932 | Bulnesol | C15H26O | 4.17 |
51 | 20.235 | - | Mansonone C | C15H26O2 | 0.07 |
52 | 20.342 | 20.360 | Epiglobulol | C15H26O | 0.78 |
53 | 20.621 | 20.647 | Cubenol | C15H26O | 1.74 |
54 | 20.967 | 20.982 | ι-Cadinol | C15H26O | 0.56 |
55 | 21.151 | 21.165 | δ-Cadinol | C15H26O | 1.82 |
56 | 21.494 | 21.522 | Torreyol | C15H26O | 0.38 |
57 | 21.662 | 21.684 | β-Selinenol | C15H26O | 7.12 |
58 | 21.867 | 21.887 | α-Eudesmol | C15H26O | 5.16 |
59 | 22.061 | 22.077 | Cadalene | C15H18 | 2.22 |
60 | 23.167 | 23.185 | Hedycaryol | C15H26O | 0.51 |
61 | 23.286 | 23.310 | Peruviol | C15H26O | 1.26 |
62 | 26.172 | 26.190 | Farnesol isomer a | C15H26O | 4.87 |
63 | 26.362 | 26.384 | cis-Farnesal | C15H24O | 0.46 |
64 | 26.471 | - | Tetradecanoic acid | C14H28O2 | 0.24 |
65 | 33.571 | 33.590 | Hexadecanoic acid | C16H32O2 | 0.58 |
2.2. Quantitative Analysis
2.3. Identification of Common Components
2.4. Comparison of Samples
Series No. | Retention time (min) | Compound name | Molecule structure |
---|---|---|---|
1 | 6.914 | m-Cymene | C10H16 |
2 | 9.128 | Octanoic acid | C8H16O2 |
3 | 10.719 | 4-Hydroxy-3-methylacetophenone | C9H10O2 |
4 | 11.526 | Di-epi-α-cedrene | C10H18O |
5 | 14.430 | α-Cedrene | C15H32 |
6 | 15.112 | 1 H-Benzocycloheptene, 2,4a,5,6,7,8,9,9a-octahydro-3,5,5-trimethyl-9-methylene- | C15H24 |
7 | 15.672 | Dihydrocurcumene | C15H24 |
8 | 16.541 | α-Muurolene | C15H24 |
9 | 17.512 | Eudesma-3,7(11)-diene | C15H24 |
10 | 19.513 | Caryophyllenyl alcohol | C15H26O |
11 | 20.247 | 1-Cyclohexen-1-ol, 2,6-dimethyl-, acetate | C10H16O2 |
12 | 26.490 | (E)-10-Pentadecenol | C15H30O |
13 | 34.172 | cis-7-Tetradecen-1-ol | C14H28O |
3. Experimental
3.1. Instrumentation and Materials
3.2. Extraction of the Essential Oil
3.3. Gas Chromatography-Mass Spectrometry
3.4. Data Analysis
4. Conclusions
Acknowledgments
- Sample Availability: Samples of the extracts from Radix Flemingiae Macrophyllae and Radix Flemingiae Latifolia Benth are available from the authors.
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Cheng, S.-Y.; Xie, Y.; Feng, X.-L.; Huang, L.-F. Study of the Volatile Constituents in Radix Flemingiae Macrophyllae and a Substitute by Gas Chromatography-Mass Spectrometry and Chemometric Methods. Molecules 2012, 17, 14111-14125. https://doi.org/10.3390/molecules171214111
Cheng S-Y, Xie Y, Feng X-L, Huang L-F. Study of the Volatile Constituents in Radix Flemingiae Macrophyllae and a Substitute by Gas Chromatography-Mass Spectrometry and Chemometric Methods. Molecules. 2012; 17(12):14111-14125. https://doi.org/10.3390/molecules171214111
Chicago/Turabian StyleCheng, Shen-Yu, Yan Xie, Xiao-Liang Feng, and Lan-Fang Huang. 2012. "Study of the Volatile Constituents in Radix Flemingiae Macrophyllae and a Substitute by Gas Chromatography-Mass Spectrometry and Chemometric Methods" Molecules 17, no. 12: 14111-14125. https://doi.org/10.3390/molecules171214111