Repeated silica gel column chromatography of the ethyl acetate extract of the culture broth of the fungus YL-52, followed by purification of Sephadex LH-20 column chromatography, revealed two compounds 1 and 2 (Figure 1).

Compound 1, isolated as white needle crystals, showed a quasi-molecular ion peak at m/z 251.1279 [M+H]⁺ in HRMS corresponding to the molecular formula C₁₄H₁₈O₄. The molecular formula indicated six degrees of unsaturation within the molecule. IR spectrum revealed the presence of OH groups (3419, 1239, 1120 cm⁻¹), C=O group (1657 cm⁻¹) and benzene ring (3080, 1617, 1584 cm⁻¹). UV absorption maxima at λ_max 246 nm (logε 3.75) and 314 nm (logε 3.56) disclosed the presence of a conjugated system. The ¹H-NMR displayed the presence of three aromatic hydrogen atoms (δ_H 7.44, 6.92, 6.72). Based on the multiplicity and coupling constant of each peak, the three hydrogen atoms above were considered as a system of three vicinal aromatic protons. In addition, the ¹H-NMR spectrum showed the presence of one methyl group (δ_H 1.25, d), two oxygenated methenyl groups (δ_H4.59–4.65, 3.88) and one phenolic hydroxyl group (δ_H 11.04). In the ¹³C-NMR spectrum, fourteen carbon atom signals were observed. The DEPT spectrum showed one signal for one carbonyl carbon atom, six signals for sp²-hybridized carbon atoms (3 × C and 3 × CH), and seven signals for sp³-hybridized carbon atoms (1 × CH₃, 2 × OCH and 4 × CH₂). The signals at δ_C 162.0 suggested the presence of one oxygenated aromatic carbon atom.

The UV and IR data were good agreement with those published for 3-(2-hydroxypropyl)-8-hydroxy-3,4-dihydroisocoumarin isolated from the Chinese mangrove associate Catunaregam spinosa [18], suggesting that compound 1 contained an 8-hydroxy-3,4-dihydroisocoumarin moiety. The ¹³C-NMR spectrum was very similar to that of 3-(2-hydroxypropyl)-8-hydroxy-3,4-dihydroisocoumarin, except for the presence of two additional methylenes. Moreover, the location of the hydroxyl group at C-14 was determined by the HMBC correlations between H-15 (δ_H 1.25) and C-14 (δ_C 67.8) and between the proton of 14-OH and C-14, as well as the coupling relationship between H-15 (δ_H 1.25) and H-14 (δ_C 3.88) in ¹H-NMR spectrum. The assignment of all proton signals in ¹H-NMR spectrum was carried out by HSQC and HMBC correlations. Based on the facts above, 1 was established as 3-(4-hydroxypentyl)-8-hydroxy-3,4-dihydroisocoumarin. The optical rotation ([α]_D²⁵ −40.4 (c 0.19, acetone)) showed that 1 was an optically pure chiral compound, but the configurations at C-3 and C-14 remained unsolved because the crystal of 1 was too fragile for an X-ray crystallographic determination. A similar case was observed for 3-(2-hydroxypropyl)-8-hydroxy-3,4-dihydro-isocoumarin [17].

Compound 2 was obtained as a white powder, and established as (E)-3-(2,5-dioxo-3-(propan-2-ylidene)pyrrolidin-1-yl) acrylic acid by X-ray crystallographic analysis in our previous study [18]. Herein, we further reported its other previously unpublished spectroscopic data. Compound 2 possessed a molecular formula of C₁₀H₁₁NO₄, as deduced from its quasi-molecular ion peak at m/z 210.0762 [M+H]⁺ in HR-ESI-MS. The molecular formula indicated six degrees of unsaturation. The IR spectrum revealed the presence of an OH group (3431, 1234 cm⁻¹), C=O groups (1714, 1628 cm⁻¹) and CH₃ groups (2924, 1359 cm⁻¹); The UV disclosed absorption maxima at λ_max 262 nm (logε 4.01), due to the presence of a conjugated system; The ¹H-NMR spectrum exhibited the presence of two methyl groups at δ_H 2.35 (3H, s) and 1.93 (3H, s), two olefinic protons at δ 7.78 (1H, d) and 6.91 (1H, d), and one methylene group at δ_H 3.35 (2H, s). The above findings were further supported by the DEPT spectrum. The ¹³C-NMR spectrum displayed 10 signals, of which five were assigned to quaternary carbon atoms by DEPT. Based on the large coupling constant of the signals at δ_H7.78 (1H, d, J = 14.8) and 6.91 (1H, d, J = 14.8), a trans configuration was established for the ∆⁶-double bond. In the HMBC spectrum, the correlations between H-6 and C-2, C-5, C-8 suggested that the CH=CHCO₂H group was attached to a nitrogen atom, and the correlations between H-10 and C-3, C-9, C-11 and between H-11 and C-3, C-9, C-10 suggested the occurrence of one propan-2-ylidene moiety at the C-3 position. From the foregoing, it was concluded that compound 2 was (E)-3-(2,5-dioxo-3-(propan-2-ylidene)pyrrolidin-1-yl) acrylic acid, in agreement with its X-ray crystallographic analysis result [19].

The antimicrobial activities of 1 and 2 were determined against pathogenic bacteria and fungi as listed in Table 1. The results showed that 1 had obvious antibacterial activities against Staphylococcus aureus and Escherichia coli, with minimum inhibitory concentration (MIC) values of 2.5 µg mL⁻¹ and 5.0 µg mL⁻¹, respectively, but 1 was less active than the standard drug ceftriaxone sodium (a broad-spectrum antibiotic) with an MIC value of 0.625 µg mL⁻¹. For plant pathogenic fungi, 1 disclosed significant growth inhibitions of 92.6 ± 2.1%, 97.3 ± 3.3%, 87.2 ± 2.8% and 94.9 ± 1.9% at 50 µg mL⁻¹ against Alternaria solani, Valsa mali, Curvularia lunata and Botryosphaeria berengeriana, respectively. Under the same conditions, the growth inhibitions of thiabendazole, a broad-spectrum antifungal drug, were 54.7 ± 2.6%, 100 ± 0.0%, 51.5 ± 4.1, compound 2 was inactive for both the tested bacteria and fungi, although it showed slight antifung% and 92.8 ± 3.7%. Obviously, 1 had higher activities than thiabendazole against phytopathogenic fungi. Under the same conditions al activities at a concentration of 100 µg mL⁻¹.

Optical rotations: Horiba SEPA-300 digital polarimeter. IR spectra: Nexus FT-IR 400 spectrometer; with KBr pellets; in cm⁻¹. UV spectra: SP-2100 UV/VIS spectrometer; λ_max (logε) in nm. NMR spectra were recorded on Bruker AV-400 spectrometers with TMS as an internal standard. MS spectra were recorded with a VG Autospec-3000 spectrometer. HR-ESI-MS were recorded with an API QSTAR Pulsar 1 spectrometer; in m/z. Column chromatography was carried out on silica gel (200–300 mesh, Qingdao Marine Chemical Inc., Qingdao, China) and Sephadex LH-20 (Pharmacia, New Jersey, NJ, USA). Fractions were monitored by TLC and spots were visualized by heating silica gel plates sprayed with 10% H₂SO₄ in ethanol.

The fungal strain was isolated in the rhizosphere soil of Stellera chamaejasme L. collected in the Qinling Mountains of Taibai town in Shaanxi Province, China, in August 2007 and deposited in the Laboratory of Natural Product Research, Northwest A&F University, Shaanxi Province, China (culture collection number YL-52). The fungus was identified as Penicillium verruculosum YL-52 (the revised name: Talaromyces verruculosus) by the streak plate method, morphological characteristics, sequence analysis of ITS and comparison analysis with GenBankB data [16].

Cultures of YL-52 maintained on potato-dextrose-agar (PDA) medium slants were subcultured in Petri dishes prior to testing. Two fungal cakes (d = 13 mm) were added to a 250 mL conical flask containing 100 mL potato-dextrose broth (PDB), and cultured for 9 d at 25 °C with a 150 r/min frequency in a rocking bed. A 60 L culture broth was obtained from 600 fermentation flasks.

The culture broth (60 L) was extracted with ethyl acetate (50 L × 3). The combined ethyl acetate solution was concentrated under vacuum to yield 12.8 g of crude extract. Ten grams of the extract were subjected to column chromatography over silica gel using CHCl₃-CH₃OH (stepwise gradient: 100:1, 50:1, 25:1, 10:1, 5:1, 1:1, 0:1, v/v) to furnish the corresponding fractions A-1 (0.8 g), A-2 (3.80 g), A-3 (1.44 g), A-4 (1.21 g), A-5 (0.32 g), A-6 (0.30 g) and A-7 (1.95 g). Fractions A-2 and A-3 were combined and subjected to column chromatography over silica gel using petroleum ether-EtOAc (stepwise gradient: 20:1, 15:1, 10:1, 5:1, 1:1, 0:1; petroleum ether: b.p. 60–90 °C) to obtain corresponding subfractions B-1 (0.57 g), B-2 (0.76 g), B-3 (0.97 g), B-4 (0.86 g), B-5 (0.94 g) and B-6 (0.90 g). Subfractions B-3 and B-4 were combined, and subjected to column chromatography on SiO₂ with petroleum ether-acetone (stepwise gradient: 10:1, 8:1, 5:1, 2:1, 1:1, 0:1). The petroleum ether–acetone (5:1) eluate was sequentially subjected to preparative silica gel TLC using petroleum ether–acetone (2:1) and Sephadex LH-20 column chromatography with methanol. Compound 1 (16 mg) was obtained as colorless needle-like crystals (petroleum ether–EtOAc, 1:1). Fractions A-5 and A-6 were combined and repeatedly subjected to column chromatography over silica gel using petroleum ether–acetone mixture (stepwise gradient: 5:1, 2:1, 1:1, 0.5:1). The petroleum ether-acetone (0.5:1) eluate was purified by preparative silica gel TLC (CHCl₃–CH₃OH, 1:1）and Sephadex LH-20 column chromatography using methanol to provide 2 (14 mg) as a white powder.

3-(4-Hydroxypentyl)-8-hydroxy-3,4-dihydroisocoumarin (1). White needle-like crystals (petroleum ether–ethyl acetate), R_f = 0.35 (petroleum ether–acetone, 2:1), 0.56 (CHCl₃–CH₃OH, 8:1), m.p. 80–81 °C, [α]_D²⁵ −40.4 (c 0.19, acetone); UV (95% EtOH): λ_max (logε) = 212 (4.30), 246 (3.75), 314 (3.56) nm; IR (KBr): ν_max = 3419, 3080, 2946, 1657, 1617, 1584, 1462, 1239, 1120 cm⁻¹; ¹³C-NMR (100 MHz, CDCl₃) δ ppm: 169.8 (C-1), 162.0 (C-8), 139.3 (C-10), 136.1 (C-6), 117.9 (C-5), 116.1 (C-7), 108.3 (C-9), 79.6 (C-3), 67.8 (C-14), 38.7 (C-13), 34.7 (C-11), 32.8 (C-4), 23.6 (C-15), 21.1 (C-12); ¹H-NMR (400 MHz, CDCl₃) δ ppm: 11.04 (1H, s, 8-OH), 7.44 (1H, t-like, J = 8.0 Hz, H-6), 6.92 (1H, d, J = 8.4 Hz, H-7), 6.72 (1H, d, J = 7.6 Hz, H-5), 4.59-4.65 (1H, m, H-3), 2.96-2.99 (2H, m, H-4), 1.89–2.00 (1H, m, H-11a), 1.77-1.85 (1H, m, H-11b), 1.60–1.77 (2H, m, H-12), 1.50–1.56 (2H, m, H-13), 3.88 (1H, hepta, J = 6.4, 11.6, 17.6 Hz, H-14), 1.25 (3H, d, J = 6.4 Hz, H-15), 1.69 (1H, s, 14-OH); Main HMBC correlations: δ_H 2.96–2.99 (H-4) / δ_C 79.6 (C-3), 117.9 (C-5), 108.3 (C-9), 139.3 (C-10); δ_H 6.72 (H-5) / δ_C 32.8 (C-4), 108.3 (C-9); δ_H7.44 (H-6) / δ_C 116.1 (C-7), 139.3 (C-10); δ_H 6.92 (H-7) / δ_C 117.9 (C-5), 108.3 (C-9); δ_H1.89-2.00 (H-11a), 1.77-1.85 (H-11b) / δ_C 79.6 (C-3); δ_H 1.50–1.56 (H-13) / δ_C 23.6 (C-15); δ_H 1.25 (H-15) / 67.8 (C-14), 38.7 (C-13); δ_H1.69 (14-OH) / 67.8 (C-14), 23.6 (C-15); (+)-ESI MS m/z = 251.0 [M+H]⁺; (+)-ESI HR MS m/z = 251.1279 [M+H]⁺ (calcd. for C₁₄H₁₉O₄, 251.1283).

(E)-3-(2,5-Dioxo-3-(propan-2-ylidene)pyrrolidin-1-yl)acrylic acid (2). White powders, R_f = 0.54 (chloroform–methanol–EtOAc, 2:3:3), 0.76 (chloroform–methanol, 1:1), m.p. 194–195 °C; UV (95%EtOH): λ_max (logε) = 262 (4.01) nm; IR (KBr): ν_max = 3431, 2924, 1714, 1628, 1359, 1234 cm⁻¹; ¹³C-NMR (100 MHz, CDCl₃) δ ppm: 173.9 (C-8), 170.9 (C-5), 168.3 (C-2), 154.1 (C-9), 133.2 (C-6), 119.3 (C-3), 110.9 (C-7), 48.6 (C-4), 24.4 (C-11), 21.0 (C-10); ¹H-NMR (400 MHz, CDCl₃) δ ppm: 7.78 (1H, d, J =14.8 Hz, H-6), 6.91 (1H, d, J = 14.8 Hz, H-7), 3.35 (2H, s, H-4), 2.35 (3H, s, H-10), 1.93 (3H, s, H-11); Main HMBC correlations: δ_H 7.78 (H-6) / δ_C 173.9 (C-8), 170.9 (C-5), 168.3 (C-2); δ_H 6.91 (H-7) / δ_C 133.2 (C-6); δ_H 2.35 (H-10) / δ_C 154.1 (C-9), 119.3 (C-3), 24.4 (C-11); δ_H 1.93 (H-11) / δ_C 154.1 (C-9), 119.3 (C-3), 21.0 (C-10); (+)-ESI MS m/z = 209.9 [M+H]⁺; (+)-ESI HR MS m/z = 210.0762 [M+H]⁺ (calcd. for C₁₀H₁₂NO₄, 210.0766).

The antibacterial activity was determined by method reported in the literature [20]. A LB (Luria-Bertani) broth (50 mL) was used for culturing E. coli and S. aureus. Half milliliter of the 24 h cultured fresh broth at 37 °C was diluted by 50 mL of the same broth to indicate a 0.5 McFarland (McF) turbidity (ca. 1.5 × 10⁸colony forming unit). A tested compound was dissolved in dimethylsulfoxide (DMSO) to provide a sample solution of 0.8 mg mL⁻¹, and then double-fold serial dilutions (0.625–160 μg mL⁻¹) were made by adding Mueller-Hinton broth. Each sample solution (100 μL) was completely mixed with the bacterial suspension (100 μL) in a well of 96-well polystyrene plate. After incubation of the plates for 24 h at 37 °C, the absorbance of each well was measured at 630 nm with a microtiter plate reader (BioRad). According to the same, were prepared and used as positive control. Five percent DMSO in the same broth served method as the tested compounds, the double-fold serial solutions of ceftriaxone sodium (0.156–2.50 μg mL⁻¹), a broad spectrum antibiotics as negative control. All experiments were performed in triplicate. The minimal inhibitory concentration (MIC, μg mL⁻¹) was defined as the concentration of a compound required to reduce the absorbance to less than 50% of that of the negative control.

The antifungal activity in vitro was assayed by the growth rate method [21] with slight modifications. The tested pathogenic fungi were Alternaria solani, Valsa mali, Curvularia lunata and Botryosphaeria berengeriana, provided by the Institute of Pesticides, Northwest A&F University. Cultures of the test fungi maintained on potato-dextrose-agar (PDA) medium slants were subcultured in Petri dishes prior to testing. Sample solution (1 mg mL⁻¹) in acetone was completely mixed with the autoclaved PDA medium to provide the medium containing 50 µg mL⁻¹ of sample, and poured into Petri dishes. Five percent acetone in PDA medium served as negative control. PDA medium containing 50 (or 100) µg mL⁻¹ of thiabendazole, a broad-spectrum antifungal drug, was used as positive control. When the medium in the plates was partially solidified, a 5-mm thick and 4 mm diameter disc of fungus cut from earlier subcultured Petri dishes was placed at the centre of the semi-solid medium. The treated and control dishes were kept in an incubator at 26 (±2) °C for 72 h. The diameters (in mm) of inhibition zones were measured in three different directions. Growth inhibition rates were calculated according to the following formula and expressed as means ± SD: %Growth inhibition rate = (d_c − d_s) / (d_c − d₀) × 100 where d₀: Diameter of the fungus cut, d_c: Diameter of the untreated control fungus, d_s: Diameter of the sample-treated fungus.