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Antioxidant Phenolic Compounds from Pu-erh Tea
State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875, China
China Academy of Chinese Medical Sciences, Beijing 100700, China
Qingdao Huaren Pharmaceutical Co., Ltd., Qingdao 266101, China
National Institutes for Food and Drug Control, Beijing 100050, China
Analytical and Testing Center, Beijing Normal University, Beijing 100875, China
Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, China
* Authors to whom correspondence should be addressed.
Received: 28 August 2012; in revised form: 18 November 2012 / Accepted: 23 November 2012 / Published: 27 November 2012
Abstract: Eight compounds were isolated from the water extract of Pu-erh tea and their structures were elucidated by NMR and MS as gallic acid (1), (+)-catechin (2), (−)-epicatechin (3), (−)-epicatechin-3-O-gallate (4), (−)-epigallocatechin-3-O-gallate (5), (−)-epiafzelechin- 3-O-gallate (6), kaempferol (7), and quercetin (8). Their in vitro antioxidant activities were assessed by the DPPH and ABTS scavenging methods with microplate assays. The relative order of DPPH scavenging capacity for these compounds was compound 8 > compound 7 > compound 1 > compound 6 > compound 4 ≈ compound 5 > compound 2 > VC (reference) > compound 3, and that of ABTS scavenging capacity was compound 1 > compound 2 > compound 7 ≈ compound 8 > compound 6 > compound 5 > compound 4 > VC (reference) > compound 3. The results showed that these phenolic compounds contributed to the antioxidant activity of Pu-erh tea.
Keywords: Pu-erh tea; DPPH˙; ABTS˙+; chemical constituent; antioxidant activity
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Zhang, H.M.; Wang, C.F.; Shen, S.M.; Wang, G.L.; Liu, P.; Liu, Z.M.; Wang, Y.Y.; Du, S.S.; Liu, Z.L.; Deng, Z.W. Antioxidant Phenolic Compounds from Pu-erh Tea. Molecules 2012, 17, 14037-14045.
Zhang HM, Wang CF, Shen SM, Wang GL, Liu P, Liu ZM, Wang YY, Du SS, Liu ZL, Deng ZW. Antioxidant Phenolic Compounds from Pu-erh Tea. Molecules. 2012; 17(12):14037-14045.
Zhang, Hai M.; Wang, Cheng F.; Shen, Sheng M.; Wang, Gang L.; Liu, Peng; Liu, Zi M.; Wang, Yong Y.; Du, Shu S.; Liu, Zhi L.; Deng, Zhi W. 2012. "Antioxidant Phenolic Compounds from Pu-erh Tea." Molecules 17, no. 12: 14037-14045.