Next Article in Journal
Wild Argentinian Amaryllidaceae, a New Renewable Source of the Acetylcholinesterase Inhibitor Galanthamine and Other Alkaloids
Previous Article in Journal
New Triterpenes from Maytenus robusta: Structural Elucidation Based on NMR Experimental Data and Theoretical Calculations
Molecules 2012, 17(11), 13457-13472; doi:10.3390/molecules171113457
Article

Antioxidant Ability and Mechanism of Rhizoma Atractylodes macrocephala

1,†,* , 1,†, 1, 1, 1, 1, 1, 1, 1 and 2,*
1 School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China 2 School of Basic Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 18 October 2012 / Revised: 7 November 2012 / Accepted: 8 November 2012 / Published: 13 November 2012
(This article belongs to the Section Medicinal Chemistry)
Download PDF [1148 KB, 18 June 2014; original version 18 June 2014]

Abstract

Rhizoma Atractylodes macrocephala (AM) has been used in Traditional Chinese Medicine (TCM) for about 2,000 years. In the study, we firstly determined the antioxidant levels of five AM extracts by •OH-scavenging, •O2-scavenging, Fe2+-chelating, Cu2+-chelating, DPPH·-scavenging, and ABTS+·-scavenging assays. After measurement of the chemical contents in five AM extracts, we quantitatively analyzed the correlations between antioxidant levels and chemical contents. It was observed that total phenolics and total flavonoids had significant positive correlations with antioxidant levels (R = 0.685 and 0.479, respectively). In contrast, total sugars and total saponins presented lower correlations with antioxidant levels (R = −0.272 and 0.244, respectively). It means that antioxidant activity of AM should be attributed to total phenolics (including phenolic acids and flavonoids), and not total sugars and total saponins. Further analysis indicated that phenolic acids exhibited higher R values with radical-scavenging assays (R = 0.32–1.00), while flavonoids showed higher R values with metal-chelating assays (R= 0.86 and 0.90). In conclusion, AM exerts its antioxidant effect through metal-chelating, and radical-scavenging which is via donating hydrogen atom and donating electron. Its metal-chelating may result from flavonoids, while its radical-scavenging can be attributed to phenolic acids, especially caffeic acid, ferulic acid, and protocatechuic acid.
Keywords: Rhizoma Atractylodes macrocephala; baizhu; antioxidant activity; metal chelating; radical-scavening; phenolic acid Rhizoma Atractylodes macrocephala; baizhu; antioxidant activity; metal chelating; radical-scavening; phenolic acid
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplement

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Li, X.; Lin, J.; Han, W.; Mai, W.; Wang, L.; Li, Q.; Lin, M.; Bai, M.; Zhang, L.; Chen, D. Antioxidant Ability and Mechanism of Rhizoma Atractylodes macrocephala. Molecules 2012, 17, 13457-13472.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

Cited By

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert