Molecules 2012, 17(11), 13045-13061; doi:10.3390/molecules171113045
Article

Sol-gel Entrapped Candida antarctica lipase B — A Biocatalyst with Excellent Stability for Kinetic Resolution of Secondary Alcohols

Received: 26 September 2012; in revised form: 15 October 2012 / Accepted: 30 October 2012 / Published: 2 November 2012
(This article belongs to the Special Issue Biocatalysis and Biotransformations in Organic Synthesis)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Sol-gel entrapment is an efficient immobilization technique that allows preparation of robust and highly stable biocatalysts. Lipase from Candida antarctica B was immobilized by sol-gel entrapment and by sol-gel entrapment combined with adsorption on Celite 545, using a ternary silane precursor system. After optimization of the immobilization protocol, the best enzyme loading was 17.4 mg/g support for sol-gel entrapped lipase and 10.7 mg/g support for samples obtained by entrapment and adsorption. Sol-gel immobilized enzymes showed excellent values of enantiomeric ratio E and activity when ionic liquid 1-octyl-3-methyl-imidazolium tetrafluoroborate was used as additive. Immobilization increased the stability of the obtained biocatalysts in several organic solvents. Excellent operational stability was obtained for the immobilized lipase, maintaining unaltered catalytic activity and enantioselectivity during 15 reuse cycles. The biocatalysts were characterized using scanning electron microscopy (SEM) and fluorescence microscopy. The improved catalytic efficiency of entrapped lipases recommends their application for large-scale kinetic resolution of optically active secondary alcohols.
Keywords: lipase; sol-gel immobilization; ionic liquids; additive; reusability; stability; kinetic resolution
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MDPI and ACS Style

Ursoiu, A.; Paul, C.; Kurtán, T.; Péter, F. Sol-gel Entrapped Candida antarctica lipase B — A Biocatalyst with Excellent Stability for Kinetic Resolution of Secondary Alcohols. Molecules 2012, 17, 13045-13061.

AMA Style

Ursoiu A, Paul C, Kurtán T, Péter F. Sol-gel Entrapped Candida antarctica lipase B — A Biocatalyst with Excellent Stability for Kinetic Resolution of Secondary Alcohols. Molecules. 2012; 17(11):13045-13061.

Chicago/Turabian Style

Ursoiu, Anca; Paul, Cristina; Kurtán, Tibor; Péter, Francisc. 2012. "Sol-gel Entrapped Candida antarctica lipase B — A Biocatalyst with Excellent Stability for Kinetic Resolution of Secondary Alcohols." Molecules 17, no. 11: 13045-13061.

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