Molecules 2012, 17(11), 12925-12937; doi:10.3390/molecules171112925
Article

Comparative Analysis of the Main Bioactive Components of San-ao Decoction and Its Series of Formulations

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Received: 15 October 2012; in revised form: 27 October 2012 / Accepted: 29 October 2012 / Published: 1 November 2012
(This article belongs to the Section Natural Products)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A high performance liquid chromatographic (HPLC) method with diode array detection (DAD) was established for simultaneous determination of seven main bioactive components in San-ao decoction and its series of formulae (San-ao decoction, Wu-ao decoction, Qi-ao decoction and Jia-wei San-ao decoction). Seven compounds were analyzed simultaneously with a XTerra C18 column (4.6 mm × 250 mm, 5 µm) using a linear gradient elution of a mobile phase containing acetonitrile (A) and a buffer solution (0.02 mol/L potassium dihydrogen phosphate and adjusted to pH 3 using phosphoric acid) (B); the flow rate was 1.0 mL/min. The sample was detected with DAD at 210, 254 and 360 nm and the column was maintained at 30 °C. All the compounds showed good linearity (r2 > 0.9984) in the tested concentration range. The precisions were evaluated by intra-day and inter-day tests, and relative standard deviation (R.S.D.) values within the range of 0.83%–2.53% and 0.64%–2.77% were reported, respectively. The recoveries of the quantified compounds were observed to cover a range from 95.34% and 104.82% with R.S.D. values less than 2.72%. The validated method was successfully applied for the simultaneous determination of seven main bioactive components including ephedrine (1), amygdalin (2), liquiritin (3), benzoic acid (4), isoliquiritin (5), formononetin (6) and glycyrrhizic acid (7) in San-ao decoction and its series of formulae. The results also showed a wide variation in the content of the identified active compounds in these samples, which could also be helpful to illustrate the drug interactions after some herbs combined in different formulations.
Keywords: San-ao decoction; formulations; Ephedrae Herba; Armeniacae Semen Amarum; Glycyrrhizae Radix et Rhizoma; HPLC-DAD
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MDPI and ACS Style

Shu, X.; Tang, Y.; Jiang, C.; Shang, E.; Fan, X.; Ding, A. Comparative Analysis of the Main Bioactive Components of San-ao Decoction and Its Series of Formulations. Molecules 2012, 17, 12925-12937.

AMA Style

Shu X, Tang Y, Jiang C, Shang E, Fan X, Ding A. Comparative Analysis of the Main Bioactive Components of San-ao Decoction and Its Series of Formulations. Molecules. 2012; 17(11):12925-12937.

Chicago/Turabian Style

Shu, Xiaoyun; Tang, Yuping; Jiang, Chenxue; Shang, Erxing; Fan, Xinshen; Ding, Anwei. 2012. "Comparative Analysis of the Main Bioactive Components of San-ao Decoction and Its Series of Formulations." Molecules 17, no. 11: 12925-12937.

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