Molecules 2012, 17(11), 12575-12586; doi:10.3390/molecules171112575
Article

Improving the Fermentation Production of the Individual Key Triterpene Ganoderic Acid Me by the Medicinal Fungus Ganoderma lucidum in Submerged Culture

1 Hunan Provincial Key Laboratory of Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China 2 National Engineering Laboratory for Rice and By-product Further Processing, Central South University of Forestry and Technology, Changsha 410004, China
* Author to whom correspondence should be addressed.
Received: 8 October 2012; in revised form: 17 October 2012 / Accepted: 18 October 2012 / Published: 24 October 2012
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Abstract: Enhanced ganoderic acid Me (GA-Me, an important anti-tumor triterpene) yield was attained with the medicinal fungus Ganoderma lucidum using response surface methodology (RSM). Interactions were studied with three variables, viz. glucose, peptone and culture time using a Central Composite Design (CCD). The CCD contains a total of 20 experiments with the first 14 experiments organized in a fractional factorial design, with the experimental trails from 15 to 20 involving the replications of the central points. A polynomial model, describing the relationships between the yield of GA-Me and the three factors in a second-order equation, was developed. The model predicted the maximum GA-Me yield of 11.9 mg·L−1 for glucose, peptone, culture time values of 44.4 g·L−1, 5.0 g·L−1, 437.1 h, respectively, and a maximum GA-Me yield of 12.4 mg·L−1 was obtained in the validation experiment, which represented a 129.6% increase in titre compared to that of the non-optimized conditions. In addition, 11.4 mg·L−1 of GA-Me was obtained in a 30-L agitated fermenter under the optimized conditions, suggesting the submerged culture conditions optimized in the present study were also suitable for GA-Me production on a large scale.
Keywords: medicinal fungi; Ganoderma lucidum; submerged culture; response surface methodology; ganoderic acid Me

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MDPI and ACS Style

Liu, G.-Q.; Wang, X.-L.; Han, W.-J.; Lin, Q.-L. Improving the Fermentation Production of the Individual Key Triterpene Ganoderic Acid Me by the Medicinal Fungus Ganoderma lucidum in Submerged Culture. Molecules 2012, 17, 12575-12586.

AMA Style

Liu G-Q, Wang X-L, Han W-J, Lin Q-L. Improving the Fermentation Production of the Individual Key Triterpene Ganoderic Acid Me by the Medicinal Fungus Ganoderma lucidum in Submerged Culture. Molecules. 2012; 17(11):12575-12586.

Chicago/Turabian Style

Liu, Gao-Qiang; Wang, Xiao-Ling; Han, Wen-Jun; Lin, Qin-Lu. 2012. "Improving the Fermentation Production of the Individual Key Triterpene Ganoderic Acid Me by the Medicinal Fungus Ganoderma lucidum in Submerged Culture." Molecules 17, no. 11: 12575-12586.

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