- freely available
Molecules 2012, 17(10), 12187-12196; doi:10.3390/molecules171012187
Published: 17 October 2012
Abstract: Isothiocyanates and substituted pyrazoles were combined to form a series of novel isothiocyanates with highly effective herbicidal activity. The target compounds were analyzed by elemental analysis, 1H-NMR, EI-MS and IR spectroscopy. The synthesized compounds, particularly compounds 3-1 and 3-7, exhibited good herbicidal activities against four weeds. The EC50 values of compound 3-1 against Echinochloa crusgalli L., Cyperus iria L., Dactylis glomerata L., and Trifolium repens L. were 64.32, 65.83, 62.42, and 67.72 µg/mL, respectively. The EC50 values of compound 3-7 against E. crusgalli L., C. iria L., D. glomerata L., T. repens L. were 65.33, 64.90, 59.41 and 67.41 µg/mL, respectively. Compounds 3-1 and 3-7 may be further optimized as lead compounds for new herbicides.
The preparation and applications of isothiocyanates (ITCs) have been widely studied because they represent a class of important intermediates in organic synthesis. Isothiocyanates and their derivatives are extremely versatile. They can be used as pesticides and pharmaceutical intermediates to synthesize various types of heterocyclic compounds. Some isothiocyanate compounds can be directly applied as pesticides, while others can be used for the treatment of bacterial and inflammatory diseases and some cancers. Isothiocyanate compounds primarily prepared by chemists [1,2,3] and biologists [4,5] have gained widespread attention.
Recent studies have shown that some isothiocyanate compounds, specifically allylisothiocyanate (AITC) from the horseradish plant, exhibit good fumigation activities against storage pests, pathogenic microorganisms and root-knot nematode pests. Isothiocyanate compounds also inhibit seed germination in some weeds. For example, aromatic ITCs can inhibit seed germination in weeds such as Matricaria inodora, Amaranthus hybridus and Echinochloa crusgalli . Norsworthy  demonstrated that tissues from cabbage and rape plants can effectively control Vigna unguiculata crop weeds. Haramoto and Gallandt  have reviewed the effect of substituted isothiocyanates against the seed germination of different weeds.
Pyrazole derivatives with agricultural activity have been patented and widely reported in previous studies [9,10,11]. Some pyrazole derivatives are used in commercial products, such as tebufenpyrad . Studies on pyrazole amides have focused on 1-sulfonyl [13,14], aminoacyl , 5-benzylamide , and 4-amidopyrazoles . Experiments on aryl pyrazole insecticide began in 1985 [17,18], and led to the successful development of Regent, a widely used insecticide [19,20]. There are some herbicides such as pyraflufenethyl (Japan, Idametsu), JV485 (Monsanto Bayer), which structure include pyrazole rings. Isothiocyanate compounds were also used to inhibit seed germination in some weeds. The isothiocyanate group could combine with the zymoproteins, which include sulfydryl amino acids (activation of apoptosis proteins), in the weeds, causing the weeds to die. According to the concept of isosteres, we combined isothiocyanates and substituted pyrazoles to produce a series of novel substituted pyrazole aminopropylisothiocyanates, seeking compounds with high herbicidal activity. The synthetic route is shown in Scheme 1.
2. Results and Discussion
2.1. The Properties of the Target Compounds
Eight target compounds were synthesized as white solids in six steps. The substitution reaction of amino-1-alkyl-3-alkylthio-5-aminopyrazole with 1,3-dibromopropane in DMF easily occurred in the presence of KOH. The reaction product was reacted with sodium azide. Hydrazoate compounds were isolated at the end of the reaction under anhydrous conditions; further reaction of the hydrazoate compounds with triphenylphosphine and carbon disulfide in THF produced the target compounds. The activity of the amino group at position 3 was high and reaction with dibromopropane occurred readily when the N at position 1 of the 1-alkyl-3-alkylthio-5-aminopyrazole ring was connected to a phenyl group. The activity of the amino group at site 3, as well as the target compound yield, decreased significantly when the N at position 1 was bonded to rejection electronic group of the methyl. The substituents at position 5 of the pyrazole ring exhibited minimal effects on the reactivity of the 3-amino site. The reaction conditions, particularly the reaction temperature, had a significant impact on the reaction yields. The synthesized compounds were characterized by 1H-NMR, IR, MS spectroscopy, and elemental analysis. 1H-NMR spectra of all of the compounds revealed was easily obtained at reasonable resolution. H shift values of the benzene ring compounds were within the expected range of δ = 7.0 to 7.6. A strong N=C=S absorption band between 2000 cm−1 to 2200 cm−1 was observed in the IR spectrum; a -CN stretching vibration absorption peak was found in the same range. The IR spectrum of compound 3-1 exhibited a broad absorption peak at 2098 cm−1 and 2222 cm−1, attributed to -NCS and -CN, respectively. A strong absorption peak was observed at 1500 cm−1 to 1604 cm−1; this peak was attributed to the C=C stretching vibrations. The MS spectra indicated that the synthesized compounds have strong molecular ion peaks.
2.2. Biological Activities
Results of the herbicidal activities of the tested compounds against the four weeds are listed in Table 1.
|Table 1. Herbicidal activities of the samples against the four weeds *.|
|Compound||Corrected strain efficacy (%)|
|Echinochloa crusgalli L.||Cyperus iria L.||Dactylis glomerata L.||Trifolium repens L.|
|AITC (A)||25.86 f||32.59 e||40.12 e||13.33 g|
|3-1||79.39 b||86.53 a||79.26 a||75.57 b|
|3-2||65.32 c||68.59 b||64.53 b||65.78 c|
|3-3||54.62 d||57.69 c||55.62 c||56.46 d|
|3-4||58.93 d||60.23 c||59.08 b||60.68 c|
|3-5||44.62 e||42.56 d||31.25 f||34.48 f|
|3-6||63.32 c||55.36 c||46.53 d||45.12 e|
|3-7||78.69 b||70.23 b||74.33 a||73.33 b|
|3-8||52.32 d||58.96 c||56.25 c||62.39 c|
|chlorimuron ethyl ( Z)||98.32 a||92.23 a||78.25 a||95.69 a|
Values are means of four replicates; Letters a, b, c, d, e, f and g represent a significant difference at p = 0.05. * The concentration of trial compounds and control herbicide were 100 μg/mL (75 g a.i./ha) and 20 μg/mL (15 a.i. g/ha), respectively. AITC (A): Allylisothiocyanate. 3-1: 1-phenyl-3-benzylthio-4-cyanopyrazole-5-aminopropyl isothiocyanate; 3-2: 1-phenyl-3-benzylthio-4-carbethoxy pyrazole-5-aminopropyl isothiocyanate; 3-3: 1-phenyl-3-methylthio-4-cyanopyrazole-5-aminopropyl isothiocyanate; 3-4: 1-phenyl-3-methylthio-4-carbethoxypyrazole-5-aminopropyl isothiocyanate; 3-5: 1-methyl-3-benzylthio-4-cyanopyrazole-5-aminopropyl isothiocyanate; 3-6: 1-methyl-3-benzylthio-4-carbethoxypyrazole-5-aminopropyl isothiocyanate; 3-7: 1-methyl-3-methylthio-4-cyanopyrazole-5-aminopropyl isothiocyanate; 3-8: 1-methyl-3-methylthio-4-carbethoxypyrazole-5-aminopropyl isothiocyanate.
Table 1 show that the tested compounds exhibited activity against the four kinds of weeds at a concentration of 100 mg/kg. The concentration of control herbicide (Z) was 20 μg/mL. The inhibitory activity sequence in E. crusgalli L. was Z > 3-1 > 3-7 > 3-2 > 3-6 > 3-4 > 3-3 > 3-8 > 3-5 > A, whereas in C. iria L., the inhibitory activity sequence was Z > 3-1 > 3-7 > 3-2 > 3-4 > 3-8 > 3-3 > 3-6 > 3-5 > A. The inhibitory activity sequence in D. glomerata L was 3-1 > Z > 3-7 > 3-2 > 3-4 > 3-8 > 3-3 > 3-6 >A > 3-5; the inhibitory activity sequence in T. repens L. was Z > 3-1 > 3-7 > 3-2 > 3-8> 3-4 > 3-3 > 3-6 > 3-5 > A. Significant differences between the compounds and the solvent control treatment were observed against the four weeds. Compounds 3-1 and 3-7 showed strong inhibitory activities against the four weeds at the tested concentration.
2.3. Rescreening Tests
Various concentrations (40, 60, 80, 100, and 120 µg/mL) of the synthesized compounds and (0.1, 1, 10, 20, and 30 µg/mL) controls were tested separately. Compounds 3-1 and 3-7 strongly suppressed the growth of weeds within the tested concentration range; compounds 3-1 and 3-7 distinctly suppressed the growth of weeds in a dose-dependent manner. The EC50 values of compound 3-1 against E. crusgalli L., C. iria L, D. glomerata L., and T. repens L. were 64.32, 65.83, 62.42, and 67.72 µg/mL, respectively. The EC50 values of compound 3-7 against E. crusgalli L., C. iria L., D. glomerata L., and T. repens L. were 65.33, 64.90, 59.41 and 67.41 µg/mL, respectively (Table 2). Compounds 3-1 and 3-7 may be further optimized as lead compounds for new herbicides.
|Table 2. Growth suppression curve of compounds 3-1, 3-7 and Z against four weeds.|
|Weeds||Samples||Correlation model||R||EC50 (µg/mL)|
|E. crusgalli beauv L.||3-1||Y = 3.29X−4.26||0.9907||64.32|
|3-7||Y = 3.58X−5.07||0.9637||65.33|
|Z||Y = 3.63X−2.88||0.9356||10.65|
|C. iria L.||3-1||Y = 2.89X−3.16||0.9976||65.83|
|3-7||Y = 3.61X−5.16||0.9917||64.90|
|Z||Y = 2.98X−6.42||0.9456||12.89|
|D. glomerata L.||3-1||Y = 2.45X−1.85||0.9895||62.42|
|3-7||Y = 2.63X−2.31||0.9895||59.41|
|Z||Y = 2.98X−5.33||0.9763||15.23|
|T. repens L.||3-1||Y = 2.52X−2.14||0.9850||67.72|
|3-7||Y = 2.78X−2.87||0.9703||67.41|
|Z||Y = 3.46X−6.39||0.9627||9.98|
3-1: 1-phenyl-3-benzylthio-4-cyanopyrazole-5-aminopropyl isothiocyanate; 3-7: 1-methyl-3-methylthio-4-cyanopyrazole-5-aminopropyl isothiocyanate; Z: chlorimuron ethyl.
The melting points of the products were determined using an X6-type apparatus (Beijing Tech Instrument Co., Beijing, China), 1H-NMR spectra were obtained using a Bruker Avance 300 MHz spectrometer with CDCl3 as the solvent and TMS as the internal standard. MS spectra were obtained with an Agilent GC-MS (6890-5973i) instrument or Applied Biosystems Mariner API-TOF equipment. FTIR spectra were obtained using an AVATAR 330Thermo Nicolet FTIR spectrophotometer, and elemental analysis was conducted on a Vario EL III elemental analyzer.
3.2. Preparation of Intermediates
3.2.1. Synthesis of Dithioacetals
A previously described method  was used to synthesize the dithioacetals (Scheme1). Compound 1-1 was prepared by the following method: potassium hydroxide powder (0.3 mol, 16.8 g) was added to a round-bottom flask (500 mL) containing acetonitrile (125 mL). The reaction mixture was cooled to 0 °C, and malononitrile (0.15 mol, 9.9 g) was added dropwise to it. The mixture was stirred at 10 °C, and carbon disulfide (0.15 mol, 11.4 g) was added dropwise to it. The mixture was stirred at room temperature for 2 h and then subjected to vacuum filtration. The filtered solids were washed with a small amount of ether, dried, and then transferred into a 250 mL round bottom flask containing 5:1 methanol-water (120 mL). The reaction mixture was stirred at (25 ± 2) °C while benzyl chloride (0.30 mol, 38 g) was added drpwise to it. A white solid precipitated from the solution. The mixture was stirred at 50 °C for 3 h until the reaction was completed. The reaction mixture was cooled in an ice bath, producing numerous light yellow solids that were precipitated rapidly, filtered, and washed with a small amount of ether. The resulting products were decanted and oven-dried for 8 h at 55 °C. Recrystallization was carried out using ethanol to obtain the pure compound. The pure compound 1-1 weighed 38.5 g (79.7% yield), and its melting point was 85.5 °C (lit.  84–85 °C). Similar methods were used to prepare compound 1-2, (81.2% yield), melting point 78 °C (lit.  77–78.5 °C). Compounds 1-3 and 1-4 were also synthesized by similar methods. The pure compound 1-3 (23.8 g, 93.3% yield) featured a melting point of 82 °C (lit.  80–81 °C). The pure compound 1-4 was obtained at 79.2% yield and had a melting point of 56 °C (lit.  56–57 °C).
3.2.2. Synthesis of 1-Alkyl-3-alkylthio-5-aminopyrazoles 2-1 to 2-8
1-Phenyl-3-thiobenzyl-5-aminopyrazole-4-carbonitrile was prepared by the following method: a certain amount of 2-cyano-3,3-dithiobenzylacrylonitrile (0.005 mol, 1.61 g) was added to a round-bottom flask (250 mL) containing ethanol (30 mL). The flask was cooled to 0 °C. Phenyl hydrazine (0.005 mol, 0.54 g) in ethanol (10 mL) was added dropwise into the flask. The resulting solution was mixed, filtered, and heated to 80 °C to reflux for 6 h. The reaction mixture was cooled in an ice bath, and the solvent was removed by vacuum evaporation. The remaining solids were recrystallized from isopropanol to produce the pure compound 2-1. Similar methods were used to prepare compounds 2-2 to 2-8 (Scheme 1).
3.2.3. Synthesis of the Target Compounds 3-1 to 3-8
Compound 3-1 was synthesized using the following method: compound 2-1 (0.01 mol, 0.306 g) was added to a round-bottom flask (250 mL) containing N,N-dimethylformamide (50 mL). The reaction flask was heated to 25 °C. Potassium hydroxide powder (0.01 mol, 0.56 g) and 1,3-dibromopropane (0.01 mol, 2.01 g) were added dropwise into the flask through a funnel in priority order under constant pressure. The solution was stirred at room temperature for 6 h until the reaction was completed and then filtered; the filtrate was concentrated under vacuum. The remaining solid was transferred to a 100 mL reaction flask with N,N-dimethylformamide (20 mL). Sodium azide (0.015 mol, 0.84 g) in N,N-dimethylformamide (10 mL) was added to the reaction flask, and the flask was heated to 45 °C for 3 h. The reaction mixture was filtered, and the filtrate was concentrated. The remaining solid was transferred into a 100 mL reaction flask containing tetrahydrofuran (30 mL). Triphenylphosphine (0.01 mol, 2.62 g) with tetrahydrofuran (20 mL) was added into the flask. The mixture was stirred at room temperature and carbon disulfide (0.015 mol, 1.14 g) of was added to it. The reaction mixture was stirred at room temperature for 6 h until the reaction was completed. The crude target products were obtained by vacuum concentration and filtration. The concentrated mixture was applied to a silica gel column and eluted using a mixture of petroleum ether, ethyl acetate, and dichloromethane (4:2:4, v/v/v) to produce compound 3-1. Similar methods were used to synthesize compounds 3-2 to 3-8 (Scheme 1).
1-Phenyl-3-benzylthio-4-cyanopyrazole-5-aminopropyl isothiocyanate (3-1). White solid, m.p. 101–102 °C, 1H-NMR (CDCl3): δ = 2.24–2.28 (m, 2H, -CH2CH2CH2-), 3.91–3.92 (m, 4H, -CH2CH2CH2-), 4.28 (s, 2H, PhCH2-), 7.24–7.45 (m, 10H, Ph); IR (KBr) ν/cm−1: 3068 (CH), 2967, 2876 (CH2), 2222 (CN), 2098 (NCS), 1723 (C=C), 1594, 1549, 1072, 927, 760, 699 (Ph); EI-MS: 405, 347; Anal. Calcd. For C21H19N5S2: C 62.20, H 4.72, N 17.27, S 15.81; Found: C 62.18, H 4.70, N 17.31, S 15.88.
1-Phenyl-3-benzylthio-4-carbethoxypyrazole-5-aminopropyl isothiocyanate (3-2). White solid, m.p. 85–86 °C, 1H-NMR (CDCl3): δ = 1.72–1.92 (t, 3H, -CH2CH3), 2.00–2.02 (m, 2H, -CH2CH2CH2-), 3.91–3.92 (m, 4H, -CH2CH2CH2-), 4.24–4.31 (m, 4H, PhCH2-, -CH2CH3), 7.21–7.51 (m, 10H, Ph); IR (KBr) ν/cm−1: 3445 (N-), 3061, 3028 (CH), 2979, 2929 (CH2), 2097 (NCS), 1593, 1454, 1226, 1068, 1014,917, 786, 763, 695 (Ph); EI-MS: 453, 410; Anal. Calcd. For C23H24N4O2S2: C 61.04, H 5.34, N 12.38, O 7.07, S 14.17; Found: C 61.15, H 5.39, N 12.44, O 7.09, S14.15.
1-Phenyl-3-methylthio-4-cyanopyrazole-5-aminopropyl isothiocyanate (3-3). White solid, m.p. 112 °C, 1H-NMR (CDCl3): δ = 2.23–2.31 (m, 2H, -CH2CH2CH2-), 2.46–2.50 (s, 3H, -SCH3), 3.91–3.92 (m, 4H, -CH2CH2CH2-), 7.26–7.74 (m, 5H, Ph); IR (KBr) ν/cm−1: 3444 (N-H), 3054 (CH), 2926 (CH2), 2191 (CN), 2098 (NCS), 1433, 1103, 691, 638 (Ph); EI-MS: 329, 271; Anal. Calcd. For C15H15N5S2: C 54.69, H 4.59, N 21.26, S 19.46; Found: C 54.77, H 4.61, N 21.24, S 19.40.
1-Phenyl-3-methylthio-4-carbethoxypyrazole-5-aminopropyl isothiocyanate (3-4). White solid, m.p. 97 °C, 1H-NMR (CDCl3): δ = 1.36–1.39 (t, 3H, -CH2CH3), 2.08–2.16 (m, 2H, -CH2CH2CH2-), 2.46–2.50 (s, 3H, -SCH3), 3.82–3.86 (m, 4H, -CH2CH2CH2-), 4.27–4.29 (m, 2H, -CH2CH3), 7.26–7.74 (m, 5H, Ph); IR (KBr) ν/cm−1: 3055 (CH), 2977, 2882 (CH2), 2201 (CN), 2109 (NCS), 1596 (CO); 1224, 994, 930, 773, 704, 637 (Ph); EI-MS: 376, 318; Anal. Calcd. For C17H20N4O2S2: C 54.23, H 5.35, N 14.88, O 8.50, S 17.03; Found: C 54.25, H 5.37, N 14.90, O 8.51, S 17.05.
1-Methyl-3-benzylthio-4-cyanopyrazole-5-aminopropyl isothiocyanate (3-5). White solid, m.p. 85–86 °C, 1H-NMR (CDCl3): δ = 2.03–2.07 (m, 2H, -CH2CH2CH2-), 3.82 (s, 3H, -N-CH3), 3.93–3.95 (m, 4H, -CH2CH2CH2-), 4.28 (s, 2H, PhCH2-), 7.20–7.53 (m, 5H, Ph); IR (KBr) ν/cm−1: 3028 (CH), 2943 (CH2), 2228 (CN), 2111 (NCS), 1716 (C=C), 1050, 699 (Ph); EI-MS: 343, 285; Anal. Calcd. For C16H17N5S2: C 55.95, H 4.99, N 20.39, S 18.67; Found: C 56.01, H 5.01, N20.35, S 18.65.
1-Methyl-3-benzylthio-4-carbethoxypyrazole-5-aminopropyl isothiocyanate (3-6). White solid, m.p. 92–93 °C, 1H-NMR (CDCl3): δ = 1.68–1.73 (t, 3H, -CH2CH3), 2.10–2.12 (m, 2H, -CH2CH2CH2-), 3.83 (s, 3H, -N-CH3), 3.88–3.92 (m, 4H, -CH2CH2CH2-), 4.24–4.28 (m, 4H, PhCH2-; -CH2CH3), 7.24–7.62 (m, 5H, ph); IR (KBr) ν/cm−1: 3386 (N-H), 3061, 3028, 2985, 2933 (CH2), 2110 (NCS), 1632 (CO), 1048, 881, 689 (Ph); EI-MS: 390, 332; Anal. Calcd. For C18H22N4O2S2: C 55.36, H 5.68, N 14.35, O 8.19, S 16.42; Found: C 55.39, H 5.70, N 14.33, O 8.20, S 16.41.
1-Methy-3-methylthio-4-cyanopyrazole-5-aminopropyl isothiocyanate (3-7). White solid, m.p. 58–59 °C, 1H-NMR (CDCl3): δ = 2.00–2.02 (m, 2H, -CH2CH2CH2-), 2.46–2.50 (s, 3H, -SCH3), 3.91–3.92 (m, 4H, -CH2CH2CH2-), 3.84 (s, 3H, -N-CH3); IR (KBr) ν/cm−1: 3435 (N-H), 2967, 2940 (CH2), 2215 (CN), 2108 (NCS), 1499, 1062, 954, 711 (five-membered ring); EI-MS: 267, 209; Anal. Calcd. For C10H13N5S2: C 44.92, H 4.90, N 26.19, S 23.98; Found: C 45.01, H 4.92, N 26.15, S 24.00.
1-Methyl-3-methylthio-4-carbethoxypyrazole-5-aminopropyl isothiocyanate (3-8). White solid, m.p. 65–66 °C, 1H-NMR (CDCl3): δ = 1.72–1.92 (t, 3H, -CH2CH3), 2.01–2.02 (m, 2H, -CH2CH3), 2.00–2.02 (m, 2H, -CH2CH2CH2-), 2.46–2.50 (s, 3H, -SCH3), 3.82 (s, 3H, -N-CH3), 3.91–3.92 (m, 4H, -CH2CH2CH2-), 4.24–4.28 (m, 2H, -CH2CH3); IR (KBr) ν/cm−1: 3386 (N-H), 2965, 2933 (CH2), 2215 (CN), 2108 (NCS), 1590 (CO), 1065, 1019, 711, 636 (five-membered ring); EI-MS: 314, 256; Anal. Calcd. For C12H18N4O2S2: C 45.84, H 5.77, N 17.82, O 10.18, S 20.39; Found: C 45.80, H 5.79, N 17.81, O10.20, S 20.35.
3.3.1. Test weeds
Seeds of weeds, including those of Echinochloa crusgalli L., Cyperus iria L., Dactylis glomerata L., and Trifolium repens L. were used. E. crusgalli seeds from the previous year were used. C. iria, D. glomerata, and T. repens were purchased from Wuhan Fu Yue Seed Industry Co., Ltd. (Wuhan, China).
3.3.2. Experimental Treatments
Weed seeds were immersed in water for 48 h, and then incubated at 30 °C for 36 h until more than 80% of the seeds had germinated. Weeds were selected and transplanted to field plots when the growth of leaves was bent. Each plot contained 20 potted plants, and pots were placed in a greenhouse with a temperature of 25 °C to 28 °C, 60% to 80% humidity, and good light. The weeds that germinated were transferred to field plots, with each plot containing 20 strains of weeds. The stem and leaf spray method was used to test samples (100 μg/mL). Each treatment was repeated four times, with the same water (L), solvent controls (K) and the positive control [chlorimuron ethyl, (Z)].
3.3.3. Rescreening Tests
Various concentrations of the synthesized compounds were applied to weeds determine the optimal concentration of compounds necessary to kill weeds.
3.3.4. Survey Methods
After 14 d of spraying, live weeds were counted and the correction strain efficacy was calculated according to a previously described method .
3.3.5. Statistical Analysis
Experimental data of herbicidal activity were analyzed using SPSS 16.0 for Windows.
In conclusion, a series of novel herbicides, in which the common pyrazole was substituted by isothiocyanates, were designed and synthesized. Most of the compounds exhibited excellent herbicidal activities against Echinochloa crusgalli L., Cyperus iria L., Dactylis glomerata L., and Trifolium repens L., which implied that the reaction of pyrazole with the electrophilic system to obtain novel isothiocyanate analogues with high activities was feasible. Compounds 3-1 and 3-7 may be further optimized as new herbicide lead compounds.
The authors are grateful to the Natural Science Foundation (NO. 31101457) and Shaanxi Science Foundation (NO. 2012JQ3007) for financial support.
- Du, X.H. Amide Thiourea and 1,2,4-Triazoles Synthesis and Plant Growth Regulating Activity. Ph.D. Dissertation, China Agricultural University, Beijing, China, 1997.
- Wong, R.; Dolman, S.J. Isothiocyanates from Tosyl Chloride Mediated Decomposition of in situ Generated Dithiocarbamic Acid Salts. J. Org. Chem. 2007, 72, 3969–3971, doi:10.1021/jo070246n.
- Munch, H.; Hansen, J.S.; Pittelkow, M.; Christensen, J.B.; Boas, U. A new efficient synthesis of isothiocyanatesfrom amines using di-tert-butyl dicarbonate. Tetrahedron Lett. 2008, 49, 3117–3119, doi:10.1016/j.tetlet.2008.03.045.
- Ono, H.; Adachi, K.; Fuke, Y.; Shinhara, K. Purification and structural analysis of substance in wassabi that suppress the growth of MKN-28 human stomach cancer cells. J. Jpn. Soc. Food Sci. 1996, 43, 1092–1097, doi:10.3136/nskkk.43.1092.
- Murata, M.; Yamashita, N.; Inoue, S.; Kawanishi, S. Mechanism of oxidative DNA damage induced by carcinogenic allyl isothiocynate. Free Radic. Biol. Med. 2000, 28, 797–805, doi:10.1016/S0891-5849(00)00168-4.
- Petersen, J.; Belz, R.; Walker, F.; Hurle, K. Weed Suppression by Release of Isothiocyanates from Turnip-Rape Mulch. Agron. J. 2001, 93, 37–43, doi:10.2134/agronj2001.93137x.
- Norsworthy, J.K.; Brandenberger, L.; Burgos, N.R.; Riley, M. Weed suppression in Vigna unguiculata with a spring-seeded brassicaceae green manure. Crop Prot. 2005, 24, 441–447, doi:10.1016/j.cropro.2004.09.015.
- Haramoto, E.R.; Gallandt, E.R. Brassica cover cropping for weed management: A review. Renew. Agric. Food Syst. 2005, 19, 187–198.
- Chen, H.S.; Li, Z.M.; Li, J.F. Synthesis of 2-Pyrazoyl-5-substituted-1,3,4-oxadiazoles and Their Biological Activities. Chem. J. Chin. Univ. 2000, 21, 1520–1523.
- Zhang, K.S.; Mu, L.J.; Long, W.X. Synthesis and Preliminary Bio activity Studies of α-Pyrazyl-N-phenyl-α-aminophosphonates. Chem. J. Chin. Univ. 1999, 20, 741–743.
- Zhao, W.G.; Chen, H.S.; Li, Z.M.; Han, Y.F.; Yan, H.; Lai, J.Y.; Wang, S.H. Synthesis of Pyrazolyl-heterocycles and Their Fungicidal Activities. Chin. J. Chem. 2001, 22, 939–942.
- Okafa, I.; Okul, S. Pyrazole derivative, insecticidal or miticidal composition containing the same as the effective ingredient. U.S. Patent 4,950,668, 21 August 1990.
- Hiromichi, I.; Takeshi, M.; Toshiji, O.; Toshiki, N.; Masakazu, T.; Hirokazu, Y.; Masako, Y. Triazole derivative and herbicide. JP Patent 1993-163254, 29 June 1993.
- Zhao, W.G.; Cao, Y.B.; Li, Z.M.; Gao, F.W.; Wang, S.H.; Wang, J.G. Synthesis and Biological Activity of 1-Sulfonyl-3,5-diamino-1H-Pyrazole Derivatives. Chin. J. Appl. Chem. 2001, 18, 423–427.
- Fuchs, U.; Erdelen, C. Substituted pyrazoline derivatives. EP Patent 0679644, 11 November 1995.
- Yoshikawa, Y.; Tomiya, K.; Kitajima, T.; Katsuta, H.; Takahashi, O.; Inami, S. Yanase, Y.; Tomura, N.; Kishi, J.; Kawasima, H. Substituted thiopene derivative and plant disease control agent comprising the same as active ingredient. EP Patent 0841336, 9 February 1999.
- Hatton, L.R.; Parnell, E.W.; Roberts, D.A. N-Phenylpyrazole derivatives. U.S. Patent 4,496,390, 29 January 1985.
- Hatton, L.R.; Parnell, E.W.; Roberts, D.A. 4-Trifluoromethylphenylhydrazinomethylene malononitriles. U.S. Patent 4,541,963, 17 September 1985.
- Hatton, L.R.; Buntain, I.G.; Hawkins, D.W.; Parnell, E.W.; Pearson, C.J.; Roberts, D.A. Derivatives of N-phenylpyrazoles. EP Patent 0967206, 29 June 1999.
- Hatton, L.R.; Buntain, I.G.; Hawkins, D.W.; Parnell, E.W.; Pearson, C.J.; Roberts, D.A. Derivatives of N-phenylpyrazoles. EP Patent 295117, 20 August 1996.
- Zhao, W.G.; Wang, S.H.; Wang, W.Y. Improved synthesis of disulfide acetal chemical reagents. Chem. Reag. 2000, 22, 376–377.
- Liu, H.; Wang, H.Q.; Liu, Z.J. Synthesis and herbicidal activity of novel pyrazolo[3,4-d]pyrimidin-4-one derivatives containing aryloxyphenoxypropionate moieties. Bioorg. Med. Chem. Lett. 2007, 17, 2203–2209, doi:10.1016/j.bmcl.2007.01.083.
- Zou, X.W. Studies on Synthesis and Biological Activities of Six Phenmethyl Urea Compounds. M.Sc. Dissertation, Huazhong Agricultural University, Wuhan, China, 2007.
- Sample Availability: Samples of compounds 3-1~3-8 are available from the authors.
© 2012 by the authors; licensee MDPI, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).